FastQCFastQC Report
Tue 7 Mar 2017
1SN_3A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_3A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2442489
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG95550.079.041218
CACCGGC92950.076.353799
CTTCACC105250.074.209496
CCTTCAC114300.071.7832955
TGCTGCG307.5915465E-570.619561
GTGTGGC39300.069.392387
GGCTGCA707.2759576E-1268.0974351
CGGGGCA8950.067.1297794
TTCACCG115850.066.4065867
CGTAGTG20350.065.736824
GCAGCTT501.999033E-763.55761
GCCTTCA35150.063.4302564
TGTGGCA43900.063.2973338
CGGGCCC7300.062.370197
CGCTGCT609.958967E-961.7921141
ACCTTCA47350.061.560174
GGGCCCG7550.060.3049558
AGGCAGC551.3002136E-859.7422182
AACGGGC7850.058.5982485
TGTGTGG43250.057.519886
Produced by FastQC (version 0.11.5)