##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_3A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2442489 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.791090563765078 12.0 12.0 12.0 2.0 27.0 2 14.468107328221334 12.0 12.0 12.0 12.0 22.0 3 13.66319070423654 12.0 12.0 12.0 12.0 22.0 4 13.825529204020981 12.0 12.0 12.0 12.0 22.0 5 22.413152321259176 22.0 22.0 22.0 22.0 27.0 6 26.82991653186565 27.0 22.0 32.0 22.0 32.0 7 34.88365925087073 37.0 37.0 37.0 27.0 41.0 8 37.5209325405355 41.0 37.0 41.0 27.0 41.0 9 38.48495162107179 41.0 37.0 41.0 32.0 41.0 10-11 38.84386951179719 41.0 41.0 41.0 34.5 41.0 12-13 39.0269356381953 41.0 41.0 41.0 37.0 41.0 14-15 38.979903696598015 41.0 41.0 41.0 37.0 41.0 16-17 38.87611203162021 41.0 41.0 41.0 37.0 41.0 18-19 38.805631468555234 41.0 41.0 41.0 34.5 41.0 20-21 38.824301358163744 41.0 41.0 41.0 37.0 41.0 22-23 38.81070928057404 41.0 41.0 41.0 34.5 41.0 24-25 38.81804032689605 41.0 41.0 41.0 34.5 41.0 26-27 38.77521434078106 41.0 41.0 41.0 34.5 41.0 28-29 38.73263994228837 41.0 41.0 41.0 32.0 41.0 30-31 38.612890170641506 41.0 41.0 41.0 32.0 41.0 32-33 38.65564287085837 41.0 41.0 41.0 32.0 41.0 34-35 38.62872401881851 41.0 41.0 41.0 32.0 41.0 36-37 38.54820881486057 41.0 41.0 41.0 32.0 41.0 38-39 38.450610627110294 41.0 41.0 41.0 32.0 41.0 40-41 38.50106592087006 41.0 41.0 41.0 32.0 41.0 42-43 38.26744357907037 41.0 41.0 41.0 32.0 41.0 44-45 38.37267987696158 41.0 41.0 41.0 32.0 41.0 46-47 38.37838798864601 41.0 41.0 41.0 32.0 41.0 48-49 38.32007964007207 41.0 41.0 41.0 32.0 41.0 50-51 38.19653926793529 41.0 41.0 41.0 32.0 41.0 52-53 38.16903023923547 41.0 39.0 41.0 32.0 41.0 54-55 38.03116329285413 41.0 37.0 41.0 32.0 41.0 56-57 37.80490741207023 41.0 37.0 41.0 27.0 41.0 58-59 37.805678551674134 41.0 37.0 41.0 27.0 41.0 60-61 37.78517405810221 41.0 37.0 41.0 27.0 41.0 62-63 37.77895642518759 41.0 37.0 41.0 27.0 41.0 64-65 37.75323860209811 41.0 37.0 41.0 27.0 41.0 66-67 37.640601452043384 41.0 37.0 41.0 27.0 41.0 68-69 37.672125647239355 41.0 37.0 41.0 27.0 41.0 70-71 37.644691337402136 41.0 37.0 41.0 27.0 41.0 72-73 37.51203935821205 41.0 37.0 41.0 27.0 41.0 74-75 37.40564297321298 41.0 37.0 41.0 27.0 41.0 76-77 36.33019698348693 39.0 34.5 41.0 24.5 41.0 78-79 36.86161513931076 41.0 37.0 41.0 27.0 41.0 80-81 37.53645727780145 41.0 37.0 41.0 27.0 41.0 82-83 37.721317680448095 41.0 37.0 41.0 27.0 41.0 84-85 37.647934545457524 41.0 37.0 41.0 27.0 41.0 86-87 37.50245323520393 41.0 37.0 41.0 27.0 41.0 88-89 37.413144132890665 41.0 37.0 41.0 27.0 41.0 90-91 37.24135543701527 41.0 37.0 41.0 27.0 41.0 92-93 37.14134393235753 41.0 37.0 41.0 27.0 41.0 94-95 36.96957366031126 41.0 37.0 41.0 27.0 41.0 96-97 36.89724682485776 41.0 37.0 41.0 27.0 41.0 98-99 36.778044650354616 41.0 37.0 41.0 27.0 41.0 100 36.64681765199352 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3247648544416606 1101 2 -0.6722848488204498 1101 3 0.017620726158618538 1101 4 -0.08855117428671022 1101 5 -1.0872935825160575 1101 6 -0.8485030518544683 1101 7 -0.3685977547977046 1101 8 -0.08520210213126234 1101 9 -0.1541116322430085 1101 10-11 0.016051918055893566 1101 12-13 0.00999810402444723 1101 14-15 0.07214379613270694 1101 16-17 -0.164560772064398 1101 18-19 -0.0989345291354553 1101 20-21 -0.023844107889594568 1101 22-23 0.06351416585384584 1101 24-25 0.024166065221344013 1101 26-27 -0.13754751123168063 1101 28-29 -0.19424962855212868 1101 30-31 -0.1590385539336765 1101 32-33 -0.13469402337140934 1101 34-35 -0.10291940637583252 1101 36-37 -0.09020475527295702 1101 38-39 -0.006434163518981961 1101 40-41 -0.0291581590371095 1101 42-43 0.006974993718685596 1101 44-45 -0.03006271600343524 1101 46-47 0.1489692015223696 1101 48-49 0.18594352811369674 1101 50-51 0.036196496520439325 1101 52-53 0.028641811999413846 1101 54-55 -0.07568442178385482 1101 56-57 0.02121214672711602 1101 58-59 -0.015383986351899637 1101 60-61 -0.14090491286750506 1101 62-63 -0.18350868849636015 1101 64-65 -0.07560320635632678 1101 66-67 0.08334332916362541 1101 68-69 -0.08561894672489245 1101 70-71 -0.08264153353648851 1101 72-73 -0.14630123839675946 1101 74-75 -0.05401773122122222 1101 76-77 -0.47095788128492444 1101 78-79 -0.12531759551220034 1101 80-81 -0.060585521907377426 1101 82-83 -0.14865535910241334 1101 84-85 -0.2800312598751731 1101 86-87 -0.2397174437510472 1101 88-89 -0.3994578701616547 1101 90-91 -0.11402635519053206 1101 92-93 -0.02382915793067042 1101 94-95 -0.10244078740786478 1101 96-97 -0.2911603492187069 1101 98-99 -0.35846941583838543 1101 100 -0.4207776079736689 1102 1 1.6112408840181995 1102 2 0.7519633270460773 1102 3 -0.1505217228432727 1102 4 0.015331205353733779 1102 5 -0.7624875913731266 1102 6 -0.017293294697264372 1102 7 0.006577230513570953 1102 8 -0.3130638016011318 1102 9 -0.19797483286713913 1102 10-11 -0.23768968518636058 1102 12-13 -0.28983025220083647 1102 14-15 -0.23511777848570148 1102 16-17 -0.04535094235428261 1102 18-19 -0.04272534959442709 1102 20-21 -0.03868495681910389 1102 22-23 -0.09886572507063107 1102 24-25 0.08119783497468802 1102 26-27 -0.08278636685635377 1102 28-29 0.0022077812701084554 1102 30-31 -0.0647111334465933 1102 32-33 -0.05913286655844274 1102 34-35 0.05702370005042923 1102 36-37 -0.1420877516131398 1102 38-39 0.03884673392597193 1102 40-41 0.005216373336715208 1102 42-43 0.038842132065639134 1102 44-45 0.02440800079988037 1102 46-47 -0.3717058946696241 1102 48-49 -0.14227612063952222 1102 50-51 -0.1788455787691845 1102 52-53 -0.12947764679906015 1102 54-55 -0.019709959544513822 1102 56-57 0.015239595479606294 1102 58-59 -0.05696781638116022 1102 60-61 -0.06709193569987093 1102 62-63 -0.32405846681051287 1102 64-65 -0.3650845674921115 1102 66-67 -0.07429093204801518 1102 68-69 -0.3507703588182949 1102 70-71 -0.2992298927243553 1102 72-73 -0.1290020118410098 1102 74-75 -0.23245114114537557 1102 76-77 -0.10358978344732606 1102 78-79 -0.2466721626639128 1102 80-81 -0.1838218222012884 1102 82-83 -0.3182825515876573 1102 84-85 -0.3512894460999192 1102 86-87 -0.26248880081219994 1102 88-89 -0.3408746341593485 1102 90-91 -0.3693710322577459 1102 92-93 -0.27617098664837414 1102 94-95 -0.18532622528303477 1102 96-97 -0.29642011130993495 1102 98-99 -0.3987793592795086 1102 100 -0.32805736400587193 1103 1 1.697846178496384 1103 2 0.802540549904089 1103 3 0.3615781250382035 1103 4 1.1550763916871531 1103 5 2.9533757266676197 1103 6 0.30117736580392673 1103 7 -0.19828625639824793 1103 8 -0.23677982638952244 1103 9 -0.3403102727157332 1103 10-11 -0.1614336308892632 1103 12-13 -0.4252318861462143 1103 14-15 -0.18197384987173848 1103 16-17 -0.32938771453509474 1103 18-19 -0.25849178542577533 1103 20-21 -0.22688638569712793 1103 22-23 -0.06825902041806842 1103 24-25 -0.08991330304814937 1103 26-27 -0.20187512239764516 1103 28-29 -0.17534198691902247 1103 30-31 -0.010253576264219078 1103 32-33 -0.278793428973124 1103 34-35 -0.3236116992287421 1103 36-37 -0.16281112532078623 1103 38-39 -0.21194867574111953 1103 40-41 -0.2932272543368839 1103 42-43 -0.12744440648775424 1103 44-45 -0.664814055242438 1103 46-47 -0.3796778203582747 1103 48-49 -0.26341420402684435 1103 50-51 -0.25358650295818563 1103 52-53 -0.09779245477381693 1103 54-55 -0.20976897022914898 1103 56-57 -0.26092886115315395 1103 58-59 -0.42728593315247565 1103 60-61 -0.06475027924283694 1103 62-63 -0.16561333208032636 1103 64-65 -0.21331488610674398 1103 66-67 -0.2774149123459466 1103 68-69 -0.2887677631824346 1103 70-71 -0.36689740643755897 1103 72-73 -0.13186541936323692 1103 74-75 -0.25899148417568085 1103 76-77 -0.38685217074606726 1103 78-79 -0.41685013662816317 1103 80-81 -0.312033316064408 1103 82-83 -0.48089059375295307 1103 84-85 -0.6213502867818335 1103 86-87 -0.17326667749057378 1103 88-89 -0.0651589672968953 1103 90-91 -0.5078305109372678 1103 92-93 -0.2570616436375772 1103 94-95 -0.2548590579237313 1103 96-97 -0.114387522556747 1103 98-99 -0.31060040169171543 1103 100 -0.34617568274968846 1104 1 1.3072654940461916 1104 2 -0.6261388916152093 1104 3 0.16806339837694217 1104 4 0.013372690264265685 1104 5 -1.1584538416080505 1104 6 -0.490719805869702 1104 7 -0.2163393743312838 1104 8 -0.2613154806816169 1104 9 -0.07592966832219616 1104 10-11 0.021174573891556747 1104 12-13 -0.053919196482077325 1104 14-15 -0.16826910839657216 1104 16-17 -0.13974444562342114 1104 18-19 -0.009235730713548662 1104 20-21 -0.062392880892019775 1104 22-23 -0.04017555622289137 1104 24-25 -0.09458977494450238 1104 26-27 -0.15337105285099284 1104 28-29 -0.2320662327955887 1104 30-31 -0.13300198282886555 1104 32-33 -0.11691202914487064 1104 34-35 -0.14087930399518456 1104 36-37 -0.2584819077206433 1104 38-39 -0.18653581946335152 1104 40-41 -0.08596536301551083 1104 42-43 -0.11175877597317196 1104 44-45 0.03833620836292084 1104 46-47 -0.15810905203334613 1104 48-49 -0.44975804394090346 1104 50-51 -0.17683167133375122 1104 52-53 -0.1655350965750202 1104 54-55 -0.2335471653963168 1104 56-57 0.09303616708905338 1104 58-59 -0.01585185843977399 1104 60-61 -0.0037122112767846716 1104 62-63 -0.0689124189554775 1104 64-65 -0.24170899749497465 1104 66-67 -0.5527581467032476 1104 68-69 -0.47118540575341683 1104 70-71 -0.37637659341774565 1104 72-73 -0.1557565149419986 1104 74-75 -0.21567022776515898 1104 76-77 -0.2671910772542603 1104 78-79 -0.28897707774546433 1104 80-81 -0.4398027118419279 1104 82-83 -0.18742307999107055 1104 84-85 -0.07447608863135002 1104 86-87 -0.25917020579949224 1104 88-89 -0.19390785967544133 1104 90-91 -0.3933084389068142 1104 92-93 -0.4597431994736709 1104 94-95 -0.2745187829256892 1104 96-97 -0.089250759103642 1104 98-99 -0.26930964207721786 1104 100 -0.18702647288150587 1105 1 1.566770943009132 1105 2 0.35007531277814863 1105 3 -0.09901802806125204 1105 4 -0.2656193725366851 1105 5 -1.1346454696530337 1105 6 0.38822258896482964 1105 7 -0.010267626696155219 1105 8 -0.07701571837831267 1105 9 -0.053136587005411684 1105 10-11 -0.15886470895461002 1105 12-13 -0.2271968629907022 1105 14-15 -0.11946517108141563 1105 16-17 -0.1635002469386535 1105 18-19 -0.24301672078318148 1105 20-21 -0.21246560898570976 1105 22-23 -0.1145575286861984 1105 24-25 -0.1666173567918321 1105 26-27 -0.1146726115865917 1105 28-29 0.02046731727068618 1105 30-31 -0.09339984259104028 1105 32-33 -0.12206878963239376 1105 34-35 -0.1253683851055527 1105 36-37 -0.19397579759321104 1105 38-39 -0.09382927006468122 1105 40-41 -0.03580265204758604 1105 42-43 0.058105991700074355 1105 44-45 0.029359852250046004 1105 46-47 -0.03390527146255096 1105 48-49 -0.1544336056888369 1105 50-51 -0.14398695468305078 1105 52-53 -0.2732481447368187 1105 54-55 -0.15855143877654854 1105 56-57 0.07509119532561925 1105 58-59 -0.20943727282865154 1105 60-61 -0.23833529914070795 1105 62-63 -0.18218118393247096 1105 64-65 -0.2322601506626043 1105 66-67 -0.14965357628278042 1105 68-69 -0.13662585418420292 1105 70-71 -0.2926115680516048 1105 72-73 -0.33358847595586383 1105 74-75 -0.1999312877569679 1105 76-77 -0.3319410119441173 1105 78-79 -0.18612955517718177 1105 80-81 -0.30757529705957865 1105 82-83 -0.09842066432180019 1105 84-85 -0.14890063363729666 1105 86-87 -0.13827708545787942 1105 88-89 -0.07057584867107636 1105 90-91 -0.2960785568521018 1105 92-93 0.13265437647154243 1105 94-95 -0.029866313556958346 1105 96-97 0.07524083765697753 1105 98-99 -0.23488877379085693 1105 100 -0.16527448374395703 1106 1 1.275608998361987 1106 2 -0.5488724118747061 1106 3 -0.1451753723086373 1106 4 -0.27916675665420243 1106 5 -1.148695196272957 1106 6 -0.7843643162880873 1106 7 -0.009294457073600881 1106 8 -0.06459548507988444 1106 9 0.08401645295990079 1106 10-11 -0.1929933297864892 1106 12-13 0.007720805717056578 1106 14-15 0.09940915868791933 1106 16-17 -0.09496518343198801 1106 18-19 0.052744037278095846 1106 20-21 0.11609356696107653 1106 22-23 0.046342669078114795 1106 24-25 -0.1655953277511486 1106 26-27 -0.04641410084703779 1106 28-29 -0.060705520277032576 1106 30-31 0.12182071220659907 1106 32-33 -0.0490902687402297 1106 34-35 -0.09080013526683928 1106 36-37 -0.32120498210625925 1106 38-39 -0.005109133004502553 1106 40-41 -0.15803749452356186 1106 42-43 0.0575886318007548 1106 44-45 -0.15983026587976212 1106 46-47 -0.22356949515830848 1106 48-49 -0.15335912151526543 1106 50-51 -0.19093525042001858 1106 52-53 -0.23760118047187717 1106 54-55 -0.14110228112561174 1106 56-57 -0.07414619587756732 1106 58-59 -0.18104455732371605 1106 60-61 -0.20838976364360207 1106 62-63 -0.2654779255803632 1106 64-65 -0.309562095521251 1106 66-67 -0.37775292376630887 1106 68-69 -0.2389961705013377 1106 70-71 -0.4995356455921396 1106 72-73 -0.41557288747483767 1106 74-75 -0.32935047406786566 1106 76-77 -0.4281378032974814 1106 78-79 -0.263131786635455 1106 80-81 -0.4097119976740231 1106 82-83 -0.34902844587154647 1106 84-85 -0.5127822077143094 1106 86-87 -0.2819136221500287 1106 88-89 -0.42991423715633204 1106 90-91 -0.46222151824046875 1106 92-93 -0.5113551627481812 1106 94-95 -0.3842686317354165 1106 96-97 -0.26335598753841083 1106 98-99 -0.536985710030379 1106 100 -0.6748883674331623 1107 1 1.5089011901250124 1107 2 -0.34691493926633044 1107 3 -0.2275776069455091 1107 4 -0.07987915542428503 1107 5 -1.1610160139075312 1107 6 -0.34811642750024774 1107 7 -0.3120318872411971 1107 8 -0.40914357741022656 1107 9 -0.3651797706770168 1107 10-11 -0.21637148577799792 1107 12-13 -0.39634593024067755 1107 14-15 -0.2967105416282152 1107 16-17 -0.29186484130735124 1107 18-19 -0.06564083128541398 1107 20-21 -0.23755763913653993 1107 22-23 -0.26409191582509806 1107 24-25 -0.15847437153443877 1107 26-27 -0.37417318447268855 1107 28-29 -0.39364454413914984 1107 30-31 -0.38122398053083373 1107 32-33 -0.16864182562386532 1107 34-35 -0.2406782262979661 1107 36-37 -0.16201612064508453 1107 38-39 -0.2640002594173865 1107 40-41 -0.3693122335993948 1107 42-43 -0.19420492413011914 1107 44-45 -0.10424042568227776 1107 46-47 -0.28070041196571793 1107 48-49 -0.22443876293399967 1107 50-51 -0.20467350341901636 1107 52-53 -0.3116921683026348 1107 54-55 -0.4105295471599959 1107 56-57 -0.24408924728287928 1107 58-59 -0.5491908047794354 1107 60-61 -0.16875440787728735 1107 62-63 -0.551086171217193 1107 64-65 -0.3652615022165051 1107 66-67 -0.24157670792463648 1107 68-69 -0.44786369464495124 1107 70-71 -0.4318984709353444 1107 72-73 -0.3738261030133643 1107 74-75 -0.34232111197874815 1107 76-77 -0.39820557862119443 1107 78-79 -0.5719350000086223 1107 80-81 -0.5928030288116943 1107 82-83 -0.3635856326542779 1107 84-85 -0.26661920048205445 1107 86-87 -0.23399257065810986 1107 88-89 -0.2907375016316607 1107 90-91 -0.09580754742933095 1107 92-93 -0.23610484877304572 1107 94-95 -0.22893819996546227 1107 96-97 -0.3535599631133053 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2223 18-19 0.19921950810613964 2223 20-21 0.221287924360702 2223 22-23 0.37726441258293875 2223 24-25 0.4037126571991223 2223 26-27 0.35654428183110554 2223 28-29 0.4210562202933019 2223 30-31 0.18790002721335952 2223 32-33 0.39231922851917034 2223 34-35 0.2703381314990523 2223 36-37 0.2100704719532942 2223 38-39 0.36843111663655037 2223 40-41 0.2842679646713435 2223 42-43 0.4220155158989769 2223 44-45 0.32820907721423964 2223 46-47 0.3297174453240004 2223 48-49 0.1841478216919228 2223 50-51 0.29186645158277713 2223 52-53 0.28880113777329797 2223 54-55 0.3248404754062477 2223 56-57 0.43015743974291354 2223 58-59 0.4912609018711862 2223 60-61 0.32283255523039145 2223 62-63 0.4733840172600168 2223 64-65 0.48377284479336424 2223 66-67 0.3838628260476682 2223 68-69 0.2916873554950712 2223 70-71 0.6107300303356311 2223 72-73 0.4839596905772865 2223 74-75 0.5355445656001123 2223 76-77 0.6070548772521107 2223 78-79 0.626873139111531 2223 80-81 0.45864940543024346 2223 82-83 0.45776510244792945 2223 84-85 0.44377217986861694 2223 86-87 0.5006517488006352 2223 88-89 0.4246007863719754 2223 90-91 0.33578491667154253 2223 92-93 0.5399293043803155 2223 94-95 0.6985763539589627 2223 96-97 0.5765069299710532 2223 98-99 0.7171595680647229 2223 100 0.775454443596324 2224 1 1.3291140412893867 2224 2 -0.7710904663883351 2224 3 -0.0658742970398194 2224 4 -0.4184411585880188 2224 5 -0.9739327556628048 2224 6 0.48558233704191167 2224 7 0.3945944318152854 2224 8 0.09116678882192986 2224 9 0.30929110495623036 2224 10-11 0.19382004022267552 2224 12-13 0.2303235397338952 2224 14-15 0.19280959212962046 2224 16-17 0.24018672954625941 2224 18-19 0.14970577771320137 2224 20-21 0.3104727867660273 2224 22-23 0.2696191131891368 2224 24-25 0.3291948281211603 2224 26-27 0.24484350522114795 2224 28-29 0.19188137644714232 2224 30-31 0.2309784182997845 2224 32-33 0.24847537267012143 2224 34-35 0.2481173024259249 2224 36-37 0.19898110608246355 2224 38-39 0.1550717455484758 2224 40-41 0.13588890374267493 2224 42-43 0.4005343682348652 2224 44-45 0.43798064518134083 2224 46-47 0.29520540690054276 2224 48-49 0.1902080202701768 2224 50-51 0.33971011925573436 2224 52-53 0.3314154486854122 2224 54-55 0.20765240754656134 2224 56-57 0.4144490779408798 2224 58-59 0.4179266438098992 2224 60-61 0.252808035806261 2224 62-63 0.17155028980681664 2224 64-65 0.24614370592910717 2224 66-67 0.41715434471748125 2224 68-69 0.5396978780107844 2224 70-71 0.4795545436218305 2224 72-73 0.3420185937287954 2224 74-75 0.5520555024262563 2224 76-77 0.5431564786063277 2224 78-79 0.2322451826068601 2224 80-81 0.3404945122867957 2224 82-83 0.2977109473100725 2224 84-85 0.2340440821915024 2224 86-87 0.47242725473585523 2224 88-89 0.3928829204664126 2224 90-91 0.44913798830283014 2224 92-93 0.37383988601401086 2224 94-95 0.3748530236784333 2224 96-97 0.4116367189715575 2224 98-99 0.46127260454827024 2224 100 0.5965032304663112 2225 1 1.0894579662219304 2225 2 1.5353188606625299 2225 3 0.9053864254441724 2225 4 1.1657248033144416 2225 5 3.120941594947322 2225 6 0.22765031658361323 2225 7 0.29340217213988495 2225 8 0.3901680372613754 2225 9 0.21244603226483605 2225 10-11 0.2049214859961097 2225 12-13 0.1724738760070963 2225 14-15 0.19915830945876678 2225 16-17 0.1313347904378901 2225 18-19 -0.013367799669907754 2225 20-21 0.05490464613536261 2225 22-23 0.19050258546170085 2225 24-25 0.21189095393163626 2225 26-27 0.20043721872443143 2225 28-29 0.10333419751758299 2225 30-31 0.011820712206599637 2225 32-33 0.08478744226055568 2225 34-35 0.09871907037727823 2225 36-37 0.2280476962318687 2225 38-39 0.0850842299429857 2225 40-41 0.030021036963248093 2225 42-43 0.25611489257943276 2225 44-45 0.18342293506092489 2225 46-47 0.15278796499324443 2225 48-49 0.10842295321690187 2225 50-51 0.2829372345551562 2225 52-53 0.29005233403034225 2225 54-55 0.3454668330630568 2225 56-57 0.3021067437618328 2225 58-59 0.272969030342864 2225 60-61 0.32256398602977043 2225 62-63 0.30598013556414116 2225 64-65 0.31485912214271394 2225 66-67 0.3743845207071672 2225 68-69 0.4210168945626762 2225 70-71 0.48263315503498916 2225 72-73 0.4338862188747754 2225 74-75 0.36268767591906226 2225 76-77 0.38027087190503295 2225 78-79 0.32713735368334085 2225 80-81 0.42297679250105347 2225 82-83 0.17954746215040274 2225 84-85 0.2392716203494416 2225 86-87 0.3334613451873025 2225 88-89 0.34056394418676206 2225 90-91 0.4526569766641515 2225 92-93 0.1080098751405032 2225 94-95 0.4206229282332288 2225 96-97 0.3880733304652537 2225 98-99 0.5983579011533209 2225 100 0.5257910158958197 2226 1 1.2984568893582455 2226 2 1.3485307589903428 2226 3 0.8932513908016695 2226 4 1.1515914753992913 2226 5 3.0174072470593956 2226 6 -0.3864775254221975 2226 7 0.015877333161483875 2226 8 0.02119491821714803 2226 9 0.10846407481110987 2226 10-11 -0.03105632654833812 2226 12-13 -0.05896282269456066 2226 14-15 0.008459160790621922 2226 16-17 -0.010808574659534997 2226 18-19 0.004828860020843706 2226 20-21 -0.012330252375313933 2226 22-23 0.14906334698719093 2226 24-25 0.16411975698748904 2226 26-27 0.10766449791658061 2226 28-29 0.09605023059557993 2226 30-31 0.08395452775813084 2226 32-33 0.07215705032280795 2226 34-35 -0.07064537686656536 2226 36-37 0.11117842341963069 2226 38-39 -0.045551119631355164 2226 40-41 -0.041237166503030664 2226 42-43 0.1454861464144983 2226 44-45 0.17420396600552124 2226 46-47 0.021563184937576807 2226 48-49 0.1538520732364077 2226 50-51 0.1295000075807451 2226 52-53 0.11844343872993335 2226 54-55 0.23756986656773904 2226 56-57 0.09523350890395221 2226 58-59 0.14722050849065482 2226 60-61 0.07540812524995744 2226 62-63 0.21851025727005435 2226 64-65 0.21481046009504468 2226 66-67 0.2406006655351831 2226 68-69 0.16790739987588665 2226 70-71 0.11808452346458864 2226 72-73 0.16768439836560844 2226 74-75 0.21323037458009964 2226 76-77 0.3480254079393248 2226 78-79 0.2529569891751322 2226 80-81 0.11025435919548698 2226 82-83 0.12151131610330168 2226 84-85 0.21981167764671738 2226 86-87 0.1681465149457253 2226 88-89 0.16457018186886074 2226 90-91 0.1728266334900468 2226 92-93 0.029391714748769004 2226 94-95 0.43121835257847607 2226 96-97 0.23099406923437726 2226 98-99 0.2790067623191774 2226 100 0.3269544350396103 2227 1 1.2440974751166394 2227 2 1.664463572886559 2227 3 0.5825473999685897 2227 4 -0.011620234560131948 2227 5 3.0447090278703897 2227 6 0.09224380964078449 2227 7 0.35347782015412577 2227 8 0.2509827704200873 2227 9 0.176476210563564 2227 10-11 0.2695566461413321 2227 12-13 0.20064274907418422 2227 14-15 0.2826423657295578 2227 16-17 0.23237808577865593 2227 18-19 0.14318738393833286 2227 20-21 0.26062122902960994 2227 22-23 0.2683433807771891 2227 24-25 0.25535458119323806 2227 26-27 0.316751704105549 2227 28-29 0.43119764678385764 2227 30-31 0.4089224223776142 2227 32-33 0.26197309340993513 2227 34-35 0.18542534774658037 2227 36-37 0.24551136394695305 2227 38-39 0.2707833167055895 2227 40-41 0.3313653062216346 2227 42-43 0.6107803928373272 2227 44-45 0.363954089299952 2227 46-47 0.4975464071295974 2227 48-49 0.29283642827226686 2227 50-51 0.5031923949026549 2227 52-53 0.4245535931450135 2227 54-55 0.42090115177582277 2227 56-57 0.5193984841718233 2227 58-59 0.6837227798751186 2227 60-61 0.3443002495437568 2227 62-63 0.3796680773920258 2227 64-65 0.28072760786768924 2227 66-67 0.49680404355832763 2227 68-69 0.4807643636916126 2227 70-71 0.6250154606812828 2227 72-73 0.5577865414913106 2227 74-75 0.6521947502220016 2227 76-77 0.8164170707945786 2227 78-79 0.6802232465422833 2227 80-81 0.39679711835385945 2227 82-83 0.51491311107484 2227 84-85 0.5091381238537309 2227 86-87 0.5265296407809075 2227 88-89 0.5125886410384695 2227 90-91 0.3959875119648757 2227 92-93 0.5111217593626307 2227 94-95 0.590127575327152 2227 96-97 0.4827254810735653 2227 98-99 0.6859188594963399 2227 100 0.6200761522746276 2228 1 1.352325165355543 2228 2 1.6474186957080779 2228 3 -0.034287375878472304 2228 4 -0.08714284706234565 2228 5 3.1291552234939672 2228 6 0.20325230004806016 2228 7 0.2003173233815545 2228 8 0.2971016393350894 2228 9 0.3012157929555386 2228 10-11 0.2845125594033391 2228 12-13 0.22952284591921313 2228 14-15 0.13727742762932849 2228 16-17 0.26404470625595877 2228 18-19 0.15012321808399776 2228 20-21 0.12256254364299934 2228 22-23 0.11562590019379115 2228 24-25 0.23402112254616725 2228 26-27 0.11621699199979929 2228 28-29 0.1890546573845171 2228 30-31 0.24523436682506627 2228 32-33 0.19220908889703736 2228 34-35 0.18817592400902328 2228 36-37 0.2047146135891893 2228 38-39 0.1544024015056351 2228 40-41 0.24378104578755 2228 42-43 0.5147690848792195 2228 44-45 0.3406081804118628 2228 46-47 0.36216590120752556 2228 48-49 0.24173131776122148 2228 50-51 0.2947606079488665 2228 52-53 0.32607958911810186 2228 54-55 0.20872119884077023 2228 56-57 0.38099177201466716 2228 58-59 0.6468917875389408 2228 60-61 0.47379593003550724 2228 62-63 0.4814592806087319 2228 64-65 0.41197999013402864 2228 66-67 0.3493571703569529 2228 68-69 0.3994280172055582 2228 70-71 0.43823694347213404 2228 72-73 0.536086981977121 2228 74-75 0.3318152223093733 2228 76-77 0.34912857487672255 2228 78-79 0.478868773745603 2228 80-81 0.46979216886292363 2228 82-83 0.4819541926930455 2228 84-85 0.3135250886546288 2228 86-87 0.4038221893128764 2228 88-89 0.4617416888733672 2228 90-91 0.2618512067680854 2228 92-93 0.40996827051977647 2228 94-95 0.7298257820956238 2228 96-97 0.3577932087298663 2228 98-99 0.5855708728904574 2228 100 0.8124152657040398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 23.0 13 32.0 14 134.0 15 487.0 16 1645.0 17 4175.0 18 6894.0 19 8716.0 20 10332.0 21 11862.0 22 13742.0 23 15422.0 24 17968.0 25 19807.0 26 22452.0 27 25624.0 28 29018.0 29 32678.0 30 37356.0 31 42638.0 32 50296.0 33 59699.0 34 74129.0 35 95909.0 36 135285.0 37 222074.0 38 540297.0 39 962860.0 40 935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.59379817323847 20.60094339884047 18.88395566191704 33.92130276600402 2 20.612190082813818 49.88752617360521 10.859272191573185 18.641011552007782 3 37.729545557830555 18.360451162727855 23.20346171466893 20.706541564772657 4 19.905473473984937 16.53747468258813 23.684487422461267 39.87256442096566 5 18.251423036091463 34.71913281902191 26.01890939938726 21.010534745499363 6 17.286587575215282 27.831691360738986 31.424747460479864 23.45697360356587 7 20.655323319777487 26.815432945655026 35.81383580437824 16.715407930189247 8 20.591781580183167 28.1114469706926 33.932476256801976 17.364295192322256 9 20.904822908107263 28.348582122580694 27.779777104420937 22.9668178648911 10-11 19.477897341605225 30.253114753024473 28.934173296174514 21.334814609195785 12-13 23.99318891507802 26.742269873068004 27.289703249431213 21.974837962422757 14-15 21.792851472411954 27.481249659670937 28.515215421645706 22.210683446271407 16-17 20.934812807754714 29.524001950469376 28.294436535845197 21.246748705930713 18-19 21.4872001470631 29.465045697237528 29.701075419377528 19.34667873632184 20-21 22.631688412926323 28.950693329632195 29.866705643300744 18.55091261414074 22-23 21.885728042173373 30.31209147717758 28.576914778326532 19.22526570232251 24-25 22.15850306797697 28.37834684209427 26.657929677472445 22.805220412456308 26-27 20.091144729822734 26.672300264197712 30.06318554556438 23.173369460415174 28-29 19.7518336424802 28.0789625439283 30.553399841021882 21.615803972569623 30-31 20.321164571661146 29.950860624910685 28.206128234073923 21.521846569354246 32-33 20.66969390650275 29.973072550173203 28.785513465976713 20.57172007734733 34-35 21.390679753317208 29.181830501590795 29.87780088262424 19.549688862467754 36-37 21.873813965999435 28.148315099883764 28.702749531318254 21.27512140279854 38-39 21.488469859175225 29.23876587124171 28.967544303548703 20.305219966034354 40-41 20.859189335641396 29.7373726869314 29.873158281211904 19.530279696215302 42-43 20.531732506307605 29.805024590709266 29.407560939411155 20.255681963571973 44-45 20.697718597709137 30.051947009792062 28.738491759840063 20.511842632658734 46-47 19.99458738204935 27.985200051915278 28.95236773375488 23.067844832280493 48-49 20.92087211037593 28.084875714895745 30.202879112249843 20.79137306247848 50-51 21.87598388365311 30.595470440194404 28.19580763720942 19.332738038943063 52-53 20.393379678883843 30.1763455088836 28.362387840567433 21.067886971665125 54-55 22.40144814894591 28.288187537008387 28.1069232199324 21.203441094113302 56-57 21.57488987911527 28.702533880789982 29.152762721355007 20.569813518739743 58-59 21.177560040202536 27.855181604452987 29.159000046885215 21.808258308459262 60-61 19.66569286314816 30.351492506912532 29.571917447173156 20.410897182766156 62-63 20.552711328694578 31.096369266953815 27.325228822293536 21.025690582058072 64-65 19.87109859745415 30.217745273567477 28.84598771661835 21.065168412360023 66-67 20.191206592946784 30.170494114814844 29.57739011311822 20.060909179120152 68-69 20.851741809277343 30.04863072054777 29.199844912300527 19.899782557874364 70-71 19.728850365344535 30.910231325504434 29.20586336315128 20.155054945999755 72-73 20.112454958855494 30.642041786063317 28.49527674433744 20.75022651074375 74-75 20.078145694821963 29.162014649810093 30.33350406081665 20.426335594551297 76-77 19.64657773279634 30.478356299659897 28.539596288867624 21.335469678676137 78-79 19.575195630359033 29.471575921119808 28.572472588412882 22.380755860108277 80-81 20.743104911951228 29.50022344079167 28.49145428532417 21.265217361932937 82-83 20.421074567787205 28.230669616117005 29.806316425580626 21.541939390515168 84-85 20.70572272792221 28.948707650269867 28.497978905943892 21.847590715864023 86-87 21.792053106482772 29.021625071801754 28.94139953137967 20.2449222903358 88-89 21.447855036399346 28.322420285209066 28.926148695040183 21.303575983351408 90-91 22.32085794449842 28.445900882255764 28.344999711360007 20.888241461885805 92-93 22.204235106074172 28.068335210516814 29.489324209853145 20.23810547355587 94-95 22.798956310550427 28.338121481816298 28.618163684667568 20.24475852296571 96-97 21.86765221869986 28.642217017149306 28.906291901416957 20.583838862733874 98-99 22.959100327575683 29.20651843263163 28.21083329341504 19.623547946377652 100 22.42401091673289 30.32287965268216 27.042414520597635 20.21069490998731 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 7.0 2 16.5 3 23.0 4 28.5 5 32.0 6 38.5 7 51.0 8 62.5 9 68.0 10 71.5 11 94.0 12 109.5 13 109.5 14 138.0 15 188.0 16 231.0 17 301.0 18 443.0 19 695.5 20 1116.0 21 1813.5 22 2837.5 23 4273.0 24 6464.0 25 9682.5 26 13832.0 27 18520.5 28 23792.0 29 29844.5 30 36956.5 31 44716.0 32 52985.5 33 62492.0 34 73380.0 35 84904.5 36 95293.5 37 102589.0 38 107477.5 39 111994.5 40 115145.0 41 115621.0 42 113976.5 43 112332.5 44 112220.5 45 109690.0 46 101475.0 47 92208.0 48 86814.0 49 83764.5 50 80358.0 51 74813.0 52 65923.0 53 56437.5 54 48267.0 55 39733.5 56 30760.0 57 23026.5 58 16970.5 59 12739.5 60 10287.0 61 8834.5 62 7461.5 63 5906.5 64 4604.5 65 3932.0 66 3845.5 67 3433.5 68 2631.5 69 2337.5 70 2561.5 71 2410.0 72 1570.0 73 809.5 74 459.5 75 291.0 76 202.0 77 149.5 78 111.0 79 85.5 80 66.5 81 46.0 82 33.5 83 31.0 84 25.0 85 24.5 86 24.5 87 20.0 88 18.0 89 15.5 90 11.0 91 9.0 92 11.5 93 11.0 94 11.0 95 12.5 96 13.0 97 17.0 98 34.0 99 73.5 100 79.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.346089992626373 2 0.0058546834806625534 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.936567165706785E-4 30-31 0.010317344315573171 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.597786929644309E-4 40-41 0.0014534354095351095 42-43 6.141276378317364E-5 44-45 0.0 46-47 0.0019652084410615566 48-49 0.0 50-51 0.0 52-53 0.0016172027796235726 54-55 0.019447375198004987 56-57 0.011729837882586166 58-59 0.014513883174090036 60-61 1.8423829134952093E-4 62-63 0.012691971181855884 64-65 8.188368504423152E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.070638189158682E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2442489.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.08232305141665 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2278173464195 68.8903293616451 2 7.534733165636727 11.766589382909402 3 1.97341373862038 4.622661871591813 4 0.806341907984708 2.5184419739663055 5 0.4331373986159617 1.6910187142190873 6 0.25800564395470355 1.2087408024215964 7 0.1623848595386411 0.8875570942808563 8 0.11522226482603734 0.7197457683890023 9 0.08213984753304675 0.5772303099422518 >10 0.3808761744329137 5.046970227546777 >50 0.01868362410240118 0.9789544790159825 >100 0.006978465833137207 0.9602539696722648 >500 2.655625017825023E-4 0.1315060443994087 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.094184252211576E-5 0.0 4 0.0 0.0 0.0 4.094184252211576E-5 0.0 5 0.0 0.0 0.0 4.094184252211576E-5 0.0 6 0.0 0.0 0.0 4.094184252211576E-5 0.0 7 0.0 0.0 0.0 4.094184252211576E-5 0.0 8 0.0 0.0 0.0 4.094184252211576E-5 0.0 9 0.0 0.0 0.0 4.094184252211576E-5 0.0 10-11 0.0 0.0 0.0 4.094184252211576E-5 0.0 12-13 0.0 0.0 0.0 4.094184252211576E-5 0.0 14-15 0.0 0.0 0.0 8.188368504423152E-5 0.0 16-17 0.0 0.0 0.0 8.188368504423152E-5 0.0 18-19 0.0 0.0 0.0 8.188368504423152E-5 0.0 20-21 0.0 0.0 0.0 8.188368504423152E-5 0.0 22-23 0.0 0.0 0.0 8.188368504423152E-5 0.0 24-25 0.0 0.0 0.0 8.188368504423152E-5 0.0 26-27 0.0 0.0 0.0 8.188368504423152E-5 0.0 28-29 0.0 0.0 0.0 8.188368504423152E-5 0.0 30-31 0.0 0.0 0.0 8.188368504423152E-5 0.0 32-33 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 34-35 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 36-37 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 38-39 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 40-41 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 42-43 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 44-45 4.094184252211576E-5 0.0 0.0 8.188368504423152E-5 0.0 46-47 8.188368504423152E-5 0.0 0.0 8.188368504423152E-5 0.0 48-49 8.188368504423152E-5 0.0 0.0 8.188368504423152E-5 0.0 50-51 8.188368504423152E-5 0.0 0.0 8.188368504423152E-5 0.0 52-53 8.188368504423152E-5 0.0 0.0 8.188368504423152E-5 0.0 54-55 8.188368504423152E-5 0.0 0.0 8.188368504423152E-5 0.0 56-57 1.228255275663473E-4 0.0 2.047092126105788E-5 8.188368504423152E-5 0.0 58-59 2.047092126105788E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 60-61 2.2518013387163669E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 62-63 2.456510551326946E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 64-65 2.456510551326946E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 66-67 2.6612197639375244E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 68-69 3.4800566143798395E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 70-71 4.2988934648221545E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 72-73 4.708311890043313E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 74-75 5.527148740485627E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 76-77 6.345985590927943E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 78-79 8.188368504423152E-4 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 80-81 0.0010644879055750098 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 82-83 0.0013306098819687623 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 84-85 0.00135108080322982 0.0 4.094184252211576E-5 8.188368504423152E-5 0.0 86-87 0.0013920226457519358 8.188368504423152E-5 4.094184252211576E-5 8.188368504423152E-5 0.0 88 0.0013920226457519358 8.188368504423152E-5 4.094184252211576E-5 8.188368504423152E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9555 0.0 79.04121 8 CACCGGC 9295 0.0 76.35379 9 CTTCACC 10525 0.0 74.20949 6 CCTTCAC 11430 0.0 71.783295 5 TGCTGCG 30 7.5915465E-5 70.61956 1 GTGTGGC 3930 0.0 69.39238 7 GGCTGCA 70 7.2759576E-12 68.097435 1 CGGGGCA 895 0.0 67.12977 94 TTCACCG 11585 0.0 66.406586 7 CGTAGTG 2035 0.0 65.73682 4 GCAGCTT 50 1.999033E-7 63.5576 1 GCCTTCA 3515 0.0 63.430256 4 TGTGGCA 4390 0.0 63.297333 8 CGGGCCC 730 0.0 62.37019 7 CGCTGCT 60 9.958967E-9 61.792114 1 ACCTTCA 4735 0.0 61.56017 4 GGGCCCG 755 0.0 60.304955 8 AGGCAGC 55 1.3002136E-8 59.742218 2 AACGGGC 785 0.0 58.598248 5 TGTGTGG 4325 0.0 57.51988 6 >>END_MODULE