FastQCFastQC Report
Tue 7 Mar 2017
1SN_4A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_4A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3294871
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA159180.4831145134361861No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG90290.2740319727236666No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT64440.1955767008784259No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG63040.19132767261601438No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA62570.1899012131279191No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61610.18698759374797982No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG56940.17281404947265006No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG55470.16835256979711802No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT46230.1403089832652022No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45040.13669730924215243No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA45000.13657590843465495No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42870.13011131543541463No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41190.12501248152052083No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC40860.1240109248586667No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA40750.12367707263804864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA401.8189894E-1188.000013
GCTGCCT500.088.03
GCTGCCG158.971943E-488.03
GCTGCAC356.0754246E-1088.03
GCAGCAC500.088.03
CAGCTGC4700.086.127661
AGCAGCA2100.085.904762
AGCTGCC1200.084.333332
AGCTGCA1150.084.1739042
AGCAGCG1150.084.1739042
TGCTGCC900.083.1111152
CAGCAGC5250.082.971431
AGCTGCT1700.082.823532
GCTGCAA800.082.500013
CTGCTGC2400.082.51
CTGCAGC2350.082.382981
GCAGCCG700.081.714293
CAGCCCT176350.081.612691
CAGCGTA72150.081.169791
GCAGCGG600.080.6666643
Produced by FastQC (version 0.11.5)