##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_4A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3294871 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.801562185590878 12.0 12.0 12.0 2.0 27.0 2 14.701158861758168 12.0 12.0 12.0 12.0 22.0 3 13.698244028370155 12.0 12.0 12.0 12.0 22.0 4 13.777118133001261 12.0 12.0 12.0 12.0 22.0 5 22.457039137495823 22.0 22.0 22.0 22.0 27.0 6 26.888804447882784 27.0 22.0 32.0 22.0 32.0 7 35.02991710449362 37.0 37.0 37.0 27.0 41.0 8 37.64239085536278 41.0 37.0 41.0 32.0 41.0 9 38.59361565293452 41.0 41.0 41.0 32.0 41.0 10-11 38.942876671044175 41.0 41.0 41.0 37.0 41.0 12-13 39.12306718533139 41.0 41.0 41.0 37.0 41.0 14-15 39.07577201049753 41.0 41.0 41.0 37.0 41.0 16-17 38.980721551769406 41.0 41.0 41.0 37.0 41.0 18-19 38.92529419209432 41.0 41.0 41.0 37.0 41.0 20-21 38.940433783295305 41.0 41.0 41.0 37.0 41.0 22-23 38.91901018279623 41.0 41.0 41.0 37.0 41.0 24-25 38.94429660523887 41.0 41.0 41.0 37.0 41.0 26-27 38.907829168425714 41.0 41.0 41.0 37.0 41.0 28-29 38.85807456498297 41.0 41.0 41.0 37.0 41.0 30-31 38.73411432496143 41.0 41.0 41.0 32.0 41.0 32-33 38.78117762425297 41.0 41.0 41.0 32.0 41.0 34-35 38.75963201594236 41.0 41.0 41.0 32.0 41.0 36-37 38.6813254600863 41.0 41.0 41.0 32.0 41.0 38-39 38.59635126838046 41.0 41.0 41.0 32.0 41.0 40-41 38.63543170582399 41.0 41.0 41.0 32.0 41.0 42-43 38.37224203921792 41.0 41.0 41.0 32.0 41.0 44-45 38.4847015254922 41.0 41.0 41.0 32.0 41.0 46-47 38.49774953253102 41.0 41.0 41.0 32.0 41.0 48-49 38.442404421903014 41.0 41.0 41.0 32.0 41.0 50-51 38.33359333339605 41.0 41.0 41.0 32.0 41.0 52-53 38.30191910396492 41.0 41.0 41.0 32.0 41.0 54-55 38.16693324260646 41.0 41.0 41.0 32.0 41.0 56-57 37.95124968473728 41.0 37.0 41.0 29.5 41.0 58-59 37.95321030777836 41.0 37.0 41.0 29.5 41.0 60-61 37.95401595388712 41.0 37.0 41.0 32.0 41.0 62-63 37.959353036886725 41.0 37.0 41.0 32.0 41.0 64-65 37.930853135069626 41.0 37.0 41.0 32.0 41.0 66-67 37.8185352324871 41.0 37.0 41.0 27.0 41.0 68-69 37.821545517260006 41.0 37.0 41.0 27.0 41.0 70-71 37.795847849581975 41.0 37.0 41.0 27.0 41.0 72-73 37.67291071486562 41.0 37.0 41.0 27.0 41.0 74-75 37.56660169700119 41.0 37.0 41.0 27.0 41.0 76-77 36.5370785381279 39.0 34.5 41.0 24.5 41.0 78-79 37.04753752119582 41.0 37.0 41.0 27.0 41.0 80-81 37.67886451396731 41.0 37.0 41.0 27.0 41.0 82-83 37.87273234672921 41.0 37.0 41.0 32.0 41.0 84-85 37.77832576753384 41.0 37.0 41.0 27.0 41.0 86-87 37.642248057662954 41.0 37.0 41.0 27.0 41.0 88-89 37.56088751274329 41.0 37.0 41.0 27.0 41.0 90-91 37.40336283271788 41.0 37.0 41.0 27.0 41.0 92-93 37.293470670020156 41.0 37.0 41.0 27.0 41.0 94-95 37.132514140917806 41.0 37.0 41.0 27.0 41.0 96-97 37.095125727228776 41.0 37.0 41.0 27.0 41.0 98-99 36.962913419068606 41.0 37.0 41.0 27.0 41.0 100 36.84472684970064 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4928997284999035 1101 2 -0.489849333579258 1101 3 -0.016519773631953782 1101 4 0.12002343733903764 1101 5 -1.05429923486885 1101 6 -0.7397914958938294 1101 7 -0.14985475280421667 1101 8 -0.08056489733945682 1101 9 -0.10075613873146949 1101 10-11 -0.04305467038994237 1101 12-13 -0.027586632579954085 1101 14-15 0.03505444094849253 1101 16-17 -0.034302017391475204 1101 18-19 0.030321820383591103 1101 20-21 0.07318334046461672 1101 22-23 0.06534248027225686 1101 24-25 -0.004644403187292312 1101 26-27 0.10364222896007647 1101 28-29 0.09186062509880344 1101 30-31 -0.006250123908536409 1101 32-33 -0.10674979392948813 1101 34-35 0.008201767795867454 1101 36-37 0.024346037608559357 1101 38-39 -0.03703315180532485 1101 40-41 -0.02775566891963166 1101 42-43 0.1310390580592511 1101 44-45 0.01450633979810334 1101 46-47 0.06468401279878577 1101 48-49 0.04632368897593864 1101 50-51 -0.02513701128413004 1101 52-53 0.028384543121603656 1101 54-55 -0.10972045902487793 1101 56-57 0.16227986238940417 1101 58-59 0.10612558002458172 1101 60-61 0.10553637866343024 1101 62-63 0.02495275696927024 1101 64-65 -0.018961179525092575 1101 66-67 -0.01265679287811139 1101 68-69 0.14916887778727528 1101 70-71 -0.08880540271625392 1101 72-73 0.1147260612950376 1101 74-75 0.0750004614985258 1101 76-77 -0.3861527094071633 1101 78-79 -0.20645822907355438 1101 80-81 -0.28636747841285626 1101 82-83 -0.09995189720858377 1101 84-85 -0.024123224344400285 1101 86-87 -0.07164478436435218 1101 88-89 -0.18193388767144114 1101 90-91 -0.1080897597956394 1101 92-93 -0.006814247144113494 1101 94-95 -0.04884262170091347 1101 96-97 -0.14471314170275917 1101 98-99 -0.2503637593031769 1101 100 -0.3885149670176631 1102 1 1.6704219370883955 1102 2 0.7919493272150984 1102 3 -0.027576618698045507 1102 4 0.15462990333363535 1102 5 -0.8183884383774327 1102 6 -0.08428369002930225 1102 7 -0.0790122973661127 1102 8 -0.21677357978012424 1102 9 -0.21217132000157335 1102 10-11 -0.269590576607591 1102 12-13 -0.3012425396706604 1102 14-15 -0.22258280105845785 1102 16-17 -0.2341728488624355 1102 18-19 -0.1814784465654924 1102 20-21 -0.04482887817361103 1102 22-23 -0.28738938251610335 1102 24-25 -0.19082110488094628 1102 26-27 -0.13730583174450572 1102 28-29 -0.2799884528212857 1102 30-31 -0.2326529351775264 1102 32-33 -0.24172088314438867 1102 34-35 -0.15238375270739368 1102 36-37 -0.20651800483378935 1102 38-39 -0.27273647675913537 1102 40-41 -0.19775415766326176 1102 42-43 -0.09124190229316298 1102 44-45 -0.05468624835535252 1102 46-47 -0.3531613507995317 1102 48-49 -0.2774415172998843 1102 50-51 -0.25672672961630383 1102 52-53 -0.29623089314038964 1102 54-55 -0.2730229195495255 1102 56-57 -0.21352801920401987 1102 58-59 -0.18744670500453964 1102 60-61 -0.26386657542758485 1102 62-63 -0.2998273279457777 1102 64-65 -0.3654063818910984 1102 66-67 -0.330299512892843 1102 68-69 -0.3231747154821534 1102 70-71 -0.36052538328106465 1102 72-73 -0.297874981732825 1102 74-75 -0.35658535413864456 1102 76-77 -0.09556420672296895 1102 78-79 -0.2559203214495156 1102 80-81 -0.20987394431256234 1102 82-83 -0.316665586012185 1102 84-85 -0.3113302612475266 1102 86-87 -0.2513063578360928 1102 88-89 -0.32977005087384725 1102 90-91 -0.38144365285847215 1102 92-93 -0.3319297384471511 1102 94-95 -0.10381314864303448 1102 96-97 -0.21802313241220617 1102 98-99 -0.3093889965727996 1102 100 -0.5971029838222606 1103 1 1.6949723074266512 1103 2 0.9007555824590412 1103 3 0.26103474672928684 1103 4 1.1045966226203827 1103 5 3.0221803685408766 1103 6 0.0772531563532155 1103 7 -0.4430803481364123 1103 8 -0.2694965201800201 1103 9 -0.1592826135969716 1103 10-11 -0.2973993115409499 1103 12-13 -0.36379540414973377 1103 14-15 -0.3440391276667967 1103 16-17 -0.3168220786455649 1103 18-19 -0.42458534353639266 1103 20-21 -0.24052372251082943 1103 22-23 -0.05068445365522223 1103 24-25 -0.21465188074235897 1103 26-27 -0.20681236760054134 1103 28-29 -0.3310031532906166 1103 30-31 -0.22856574055889212 1103 32-33 -0.43402219983651236 1103 34-35 -0.3628266709711596 1103 36-37 -0.243886762787362 1103 38-39 -0.24729139503649122 1103 40-41 -0.3687796201716722 1103 42-43 -0.13793266028584839 1103 44-45 -0.45520581851985753 1103 46-47 -0.5550770957647089 1103 48-49 -0.3265681854796938 1103 50-51 -0.3491161998196546 1103 52-53 -0.26947923526782347 1103 54-55 -0.43048234725727497 1103 56-57 -0.3175045331364359 1103 58-59 -0.4414860825064366 1103 60-61 -0.25821310458498203 1103 62-63 -0.39281015543777187 1103 64-65 -0.4242758407373515 1103 66-67 -0.5068496588094362 1103 68-69 -0.5580825708355803 1103 70-71 -0.58568832276287 1103 72-73 -0.36056725967225844 1103 74-75 -0.5528650549437586 1103 76-77 -0.5466513341851211 1103 78-79 -0.6875167670863434 1103 80-81 -0.2908336359707917 1103 82-83 -0.4464391156464771 1103 84-85 -0.5482345343550961 1103 86-87 -0.4409549934118502 1103 88-89 -0.3397049600893354 1103 90-91 -0.6723298379196407 1103 92-93 -0.3166699151112127 1103 94-95 -0.35414786846023105 1103 96-97 -0.28181329135205857 1103 98-99 -0.3832296758903837 1103 100 -0.4218458127423972 1104 1 1.6192045229187286 1104 2 -0.4281482716342211 1104 3 -0.026917167013456478 1104 4 0.17166366477138872 1104 5 -1.1090199940115113 1104 6 -0.24288472341912382 1104 7 -0.3015784851630201 1104 8 -0.2375011001505456 1104 9 0.06442096549206866 1104 10-11 -0.12302225533931477 1104 12-13 -0.25251611092089377 1104 14-15 -0.3761508494215704 1104 16-17 0.026819049421085595 1104 18-19 -0.12458311191657145 1104 20-21 -0.08369878536097275 1104 22-23 -0.02094726122178514 1104 24-25 -0.049542913321424464 1104 26-27 -0.08229055652089556 1104 28-29 -0.14156995897575086 1104 30-31 -0.10385123769082583 1104 32-33 -0.19010572421652938 1104 34-35 -0.1776502914653264 1104 36-37 -0.13653898286284516 1104 38-39 -0.18791357064265668 1104 40-41 -0.14771836478251998 1104 42-43 -0.08896534235813647 1104 44-45 -0.07611884285395121 1104 46-47 -0.2593089335980423 1104 48-49 -0.4040251345292347 1104 50-51 -0.29923606355351495 1104 52-53 -0.16271639723105835 1104 54-55 -0.18258886762122728 1104 56-57 -0.051257697101270594 1104 58-59 -0.13157331309199094 1104 60-61 -0.18629100184978142 1104 62-63 -0.15905098326572897 1104 64-65 -0.2570228016573992 1104 66-67 -0.5859498525312006 1104 68-69 -0.45922996674983096 1104 70-71 -0.3482641441920862 1104 72-73 -0.0932848662085064 1104 74-75 -0.3168764087387501 1104 76-77 -0.3529162635071472 1104 78-79 -0.28791948863254646 1104 80-81 -0.33796973817211295 1104 82-83 -0.35361188507808805 1104 84-85 -0.29228662379498616 1104 86-87 -0.21854446634617375 1104 88-89 -0.15412903480395812 1104 90-91 -0.07051218357523226 1104 92-93 -0.3237716160346693 1104 94-95 -0.13555641539247887 1104 96-97 -0.277142768560779 1104 98-99 -0.4423933506129174 1104 100 -0.4391348581498491 1105 1 1.5531690752747895 1105 2 0.2973571140408193 1105 3 -0.08268673172279506 1105 4 -0.24162759557014546 1105 5 -1.1113436531327565 1105 6 0.49661813340716066 1105 7 0.20050437001523136 1105 8 0.03596547104804415 1105 9 0.06336103242834668 1105 10-11 0.08675149835769957 1105 12-13 0.04893038870456934 1105 14-15 0.05448719839285587 1105 16-17 0.028325208834466764 1105 18-19 0.0021440075189218533 1105 20-21 0.004107189276986389 1105 22-23 0.10993131120770983 1105 24-25 0.031376929808011766 1105 26-27 0.09106244460153334 1105 28-29 0.03054201148252389 1105 30-31 0.07648373933212582 1105 32-33 -0.02027464079198893 1105 34-35 0.08984628560381225 1105 36-37 0.08362576939889976 1105 38-39 0.11573665167908587 1105 40-41 0.19389967942857567 1105 42-43 0.217036297296751 1105 44-45 0.14575557084838664 1105 46-47 -0.16498658778356656 1105 48-49 -0.24382993319665758 1105 50-51 0.053675208398743735 1105 52-53 -0.0050067488257710124 1105 54-55 0.06539665434721087 1105 56-57 0.11872724945165913 1105 58-59 0.17838331892095027 1105 60-61 -0.08233131911043756 1105 62-63 -0.20998491616091997 1105 64-65 -0.11943634384731894 1105 66-67 0.026398862304326087 1105 68-69 -0.0367899605673756 1105 70-71 -0.10232027653393061 1105 72-73 0.012751445024242969 1105 74-75 -0.058966191249382405 1105 76-77 0.048882224211503456 1105 78-79 -0.02917896890570404 1105 80-81 -0.21590978089997037 1105 82-83 -0.13739089386435666 1105 84-85 0.017165181198741664 1105 86-87 -0.08080880087300812 1105 88-89 -0.1534186689914634 1105 90-91 -0.11908247854086085 1105 92-93 -0.0292116552709345 1105 94-95 0.005898908667475666 1105 96-97 -8.414845368918122E-4 1105 98-99 0.03163107335472404 1105 100 -0.1474580852439118 1106 1 1.199099378872278 1106 2 -0.6367356744605424 1106 3 -0.11831279257043015 1106 4 -0.25088548617144113 1106 5 -1.0944759183230417 1106 6 -0.6642450378985956 1106 7 -0.18879450608691428 1106 8 -0.09793266378217425 1106 9 -0.012698625363775307 1106 10-11 -0.03453979661663453 1106 12-13 -0.10435977774982774 1106 14-15 -0.019587767639428932 1106 16-17 -0.16655258746722978 1106 18-19 -0.009290379755611866 1106 20-21 0.031083151710909362 1106 22-23 -0.10444932080921632 1106 24-25 -0.09729819648691063 1106 26-27 -0.17026451361622463 1106 28-29 -0.3087893107117452 1106 30-31 -0.05648648989105709 1106 32-33 -0.15554790187136547 1106 34-35 -0.14756272004016324 1106 36-37 -0.16468099307144968 1106 38-39 -0.02324537162993323 1106 40-41 -0.06907315143119064 1106 42-43 -0.01757640665256588 1106 44-45 -0.04617170185392183 1106 46-47 -0.32001180787012373 1106 48-49 -0.25621487885059224 1106 50-51 -0.22685741090699452 1106 52-53 -0.19456223049922272 1106 54-55 -0.19145186221654598 1106 56-57 0.021016632440478134 1106 58-59 -0.09425269267178038 1106 60-61 -0.04188136183036306 1106 62-63 -0.12132459654173999 1106 64-65 -0.3665771749889757 1106 66-67 -0.2541486300337752 1106 68-69 -0.2865328287195439 1106 70-71 -0.40315956174972456 1106 72-73 -0.14460277752116468 1106 74-75 -0.32056223694483066 1106 76-77 -0.30635128697279157 1106 78-79 -0.046902360707598234 1106 80-81 -0.1734413815174065 1106 82-83 -0.21953076169113928 1106 84-85 -0.3614008571242593 1106 86-87 -0.16270337807388557 1106 88-89 -0.40115150402482413 1106 90-91 -0.35757984692939715 1106 92-93 -0.38527496725269117 1106 94-95 -0.24855333356800458 1106 96-97 -0.17069803528391247 1106 98-99 -0.3678804848219457 1106 100 -0.5152441497159899 1107 1 1.4173960899762807 1107 2 -0.5135088773559726 1107 3 -0.0041448523328924125 1107 4 -0.2382824673917554 1107 5 -1.2865851137126114 1107 6 -0.2284022949445763 1107 7 -0.371471253458985 1107 8 -0.3145237943202801 1107 9 -0.3257266288472067 1107 10-11 -0.19953991259890813 1107 12-13 -0.377988019235886 1107 14-15 -0.4657500773163008 1107 16-17 -0.36582651510618547 1107 18-19 -0.4062082469359751 1107 20-21 -0.459366144976066 1107 22-23 -0.19490727828323884 1107 24-25 -0.29966739383244345 1107 26-27 -0.3342413045719823 1107 28-29 -0.24633920786052954 1107 30-31 -0.4503456305241684 1107 32-33 -0.2615867084918406 1107 34-35 -0.4611903926885219 1107 36-37 -0.34829014469082864 1107 38-39 -0.3205051475661591 1107 40-41 -0.38617784449370873 1107 42-43 -0.19131826414310638 1107 44-45 -0.23042848917751257 1107 46-47 -0.5462455533038266 1107 48-49 -0.29052867233709634 1107 50-51 -0.41539988903676317 1107 52-53 -0.35708131657753484 1107 54-55 -0.45393420697485 1107 56-57 -0.31501135577292416 1107 58-59 -0.47574363492228144 1107 60-61 -0.25602410069294024 1107 62-63 -0.3864504629555725 1107 64-65 -0.318223930976373 1107 66-67 -0.44995731033382214 1107 68-69 -0.5797821823369134 1107 70-71 -0.40668466526827984 1107 72-73 -0.3176734531569778 1107 74-75 -0.48619996403258625 1107 76-77 -0.4260898151309007 1107 78-79 -0.7864068681052316 1107 80-81 -0.491861919728791 1107 82-83 -0.24171852079351197 1107 84-85 -0.4186554202691397 1107 86-87 -0.34463329284031374 1107 88-89 -0.3560133003606438 1107 90-91 -0.1887700920156874 1107 92-93 -0.25723006824062367 1107 94-95 -0.34189458711143317 1107 96-97 -0.5230433309553675 1107 98-99 -0.44554932733165487 1107 100 -0.5458593908128364 1108 1 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9 0.1958901154839765 2223 10-11 0.15283939684395165 2223 12-13 0.25307249195407877 2223 14-15 0.24343924963319097 2223 16-17 0.11659538385534063 2223 18-19 0.06499076178539553 2223 20-21 0.17931425354460373 2223 22-23 0.21402472686389018 2223 24-25 0.15973576032429548 2223 26-27 0.17039391373545953 2223 28-29 0.07185853491118621 2223 30-31 0.21576240412429115 2223 32-33 0.08437471765865467 2223 34-35 0.2248825638814651 2223 36-37 0.014974445269025694 2223 38-39 0.06448029366556085 2223 40-41 0.11076567063344811 2223 42-43 0.2023398234326521 2223 44-45 0.29710730474467795 2223 46-47 0.21980363980083695 2223 48-49 0.10693305114606488 2223 50-51 0.20436933035446714 2223 52-53 0.2068125677212862 2223 54-55 0.2913565019639037 2223 56-57 0.41470567241893264 2223 58-59 0.26931767995706224 2223 60-61 0.2739137337484294 2223 62-63 0.2339718697945159 2223 64-65 0.23897545459013259 2223 66-67 0.32125301763024794 2223 68-69 0.28722892649285825 2223 70-71 0.3353397245747729 2223 72-73 0.3575676362958333 2223 74-75 0.32356152864451104 2223 76-77 0.4186420033065943 2223 78-79 0.47693890096324054 2223 80-81 0.32482115096647846 2223 82-83 0.3259432361612866 2223 84-85 0.3434472575303502 2223 86-87 0.3516799325952462 2223 88-89 0.38338272197877643 2223 90-91 0.44143997136976054 2223 92-93 0.3982096380423954 2223 94-95 0.5326306238975391 2223 96-97 0.5609249640541876 2223 98-99 0.4739037865827527 2223 100 0.5597952074732575 2224 1 1.275603969147694 2224 2 -1.0005575037703007 2224 3 0.14010271235986593 2224 4 -0.33929079048970046 2224 5 -1.092775816316923 2224 6 0.591110283420683 2224 7 0.430722664943346 2224 8 0.14382184148814758 2224 9 0.2664581240411934 2224 10-11 0.10316846587911499 2224 12-13 0.12175378906417933 2224 14-15 0.12509091308141507 2224 16-17 0.15476669168951673 2224 18-19 0.10070622004036522 2224 20-21 0.11608825201692952 2224 22-23 0.19697876487592936 2224 24-25 0.2587031635946957 2224 26-27 0.1940673462953626 2224 28-29 0.07849392628247642 2224 30-31 0.23661109596410057 2224 32-33 0.16594138748599363 2224 34-35 0.28647332212236876 2224 36-37 0.11617585833962352 2224 38-39 0.2015081135791803 2224 40-41 0.15160008896324229 2224 42-43 0.3564460346939171 2224 44-45 0.18895240559252358 2224 46-47 0.17318778410540148 2224 48-49 0.12681810312832198 2224 50-51 0.11979338814094831 2224 52-53 0.21108210816110073 2224 54-55 0.22014283346519647 2224 56-57 0.5205066018386404 2224 58-59 0.3756555018198924 2224 60-61 0.22537467353176055 2224 62-63 0.35623405697747046 2224 64-65 0.217407864113369 2224 66-67 0.29634439954799774 2224 68-69 0.3515154541774308 2224 70-71 0.23811891495888204 2224 72-73 0.43360191476036647 2224 74-75 0.32479648457846366 2224 76-77 0.4969469109190854 2224 78-79 0.22562399087349405 2224 80-81 0.3231584144703561 2224 82-83 0.13630058818984736 2224 84-85 0.1453995509002297 2224 86-87 0.21522929788666545 2224 88-89 0.2304363912488938 2224 90-91 0.3146404862905925 2224 92-93 0.1301959610030039 2224 94-95 0.38915492892774495 2224 96-97 0.35548353561033963 2224 98-99 0.3716638878404197 2224 100 0.47965554426564694 2225 1 1.3355183638763801 2225 2 1.617624258077024 2225 3 0.8621600756261643 2225 4 1.1180988466111046 2225 5 3.1272927776063426 2225 6 0.06713964842254327 2225 7 0.17453513709362767 2225 8 0.3677766287585058 2225 9 0.17571203803404956 2225 10-11 0.2246951769890373 2225 12-13 0.17802320640370084 2225 14-15 0.2004738262013248 2225 16-17 0.2223719967422113 2225 18-19 0.2011777157617729 2225 20-21 0.22601640018640978 2225 22-23 0.24975319020071396 2225 24-25 0.2687137406046034 2225 26-27 0.25609174950103863 2225 28-29 0.24906769091422376 2225 30-31 0.05209597848263314 2225 32-33 0.1698111708036052 2225 34-35 0.23079455108899083 2225 36-37 0.12611287710547714 2225 38-39 0.14795426923141264 2225 40-41 0.23488225092839343 2225 42-43 0.39164721190122975 2225 44-45 0.25026905455433734 2225 46-47 0.24285134292669142 2225 48-49 0.21812213013808446 2225 50-51 0.11930814471651985 2225 52-53 0.21744277329746353 2225 54-55 0.25171584495692656 2225 56-57 0.5323685859167782 2225 58-59 0.5449755631497908 2225 60-61 0.46823501970534664 2225 62-63 0.32536776693054037 2225 64-65 0.3803288172308328 2225 66-67 0.4214451691783836 2225 68-69 0.42320651230646433 2225 70-71 0.3761353532182099 2225 72-73 0.3649826305429045 2225 74-75 0.41067349680095333 2225 76-77 0.4473485148330738 2225 78-79 0.3640404092571785 2225 80-81 0.39586224683191773 2225 82-83 0.33093719382458886 2225 84-85 0.2856946307717436 2225 86-87 0.32029766746302357 2225 88-89 0.3137109793542834 2225 90-91 0.31071527609016414 2225 92-93 0.2715881029857954 2225 94-95 0.4946135244151293 2225 96-97 0.577915518046332 2225 98-99 0.4756103507503653 2225 100 0.5120747624528263 2226 1 1.321987124387432 2226 2 1.3382736890454314 2226 3 0.7946540666593975 2226 4 0.9668995944697762 2226 5 3.101454077747448 2226 6 -0.3197857307462577 2226 7 0.14800968185979002 2226 8 0.14108522382238675 2226 9 0.3486865636697374 2226 10-11 0.1297054760354328 2226 12-13 0.20657409317244202 2226 14-15 0.08917388496328016 2226 16-17 0.09632034903754771 2226 18-19 0.19641085910382117 2226 20-21 0.08349387334236269 2226 22-23 0.18947513022468598 2226 24-25 0.17248963883735513 2226 26-27 0.2088173277409524 2226 28-29 0.2446182287660008 2226 30-31 0.1606373057261976 2226 32-33 0.10974274653199956 2226 34-35 0.13922417922117347 2226 36-37 0.19242885653136454 2226 38-39 0.11072945535376988 2226 40-41 0.18189866829931844 2226 42-43 0.25414200812744525 2226 44-45 0.2766241058268548 2226 46-47 0.23688235028021865 2226 48-49 0.19773755626505363 2226 50-51 0.1887192631181449 2226 52-53 0.12204739213566285 2226 54-55 0.21696965491882025 2226 56-57 0.3720841871875038 2226 58-59 0.26528378645436135 2226 60-61 0.23908537773069582 2226 62-63 0.26302692817071716 2226 64-65 0.4540989420585859 2226 66-67 0.27435439994497557 2226 68-69 0.40218286647258594 2226 70-71 0.5381681057275856 2226 72-73 0.2857252012100062 2226 74-75 0.32007952135761997 2226 76-77 0.4399950471067484 2226 78-79 0.3862883481630206 2226 80-81 0.4219570665857617 2226 82-83 0.18361508865039866 2226 84-85 0.23781180291114623 2226 86-87 0.3039302070816632 2226 88-89 0.3405947740175108 2226 90-91 0.40433706267062064 2226 92-93 0.25396505550738624 2226 94-95 0.36715267478234637 2226 96-97 0.38650835479831613 2226 98-99 0.4642943627585865 2226 100 0.5495650044846982 2227 1 1.2456569871435885 2227 2 1.9828753742562544 2227 3 0.613690454223704 2227 4 -0.1463137573222557 2227 5 3.176092167613966 2227 6 0.0941830603798941 2227 7 0.3272573189754482 2227 8 0.4249783001012517 2227 9 0.3499974731024693 2227 10-11 0.2802028354160413 2227 12-13 0.16436263852444455 2227 14-15 0.3360478701621403 2227 16-17 0.24835593330664096 2227 18-19 0.19030239715780084 2227 20-21 0.117034012466263 2227 22-23 0.1946333110173768 2227 24-25 0.40644082583422403 2227 26-27 0.2685021625526929 2227 28-29 0.19443962376506363 2227 30-31 0.2202913760401657 2227 32-33 0.25026215380268013 2227 34-35 0.20439022205219004 2227 36-37 0.21494932346807616 2227 38-39 0.2064557538719427 2227 40-41 0.30775537410526255 2227 42-43 0.48309481880615124 2227 44-45 0.3359107113063544 2227 46-47 0.32664631821676693 2227 48-49 0.24434918866348454 2227 50-51 0.38204934908782917 2227 52-53 0.2564685922929115 2227 54-55 0.5084803626731826 2227 56-57 0.4201203867465466 2227 58-59 0.46921480561380235 2227 60-61 0.43189075809952726 2227 62-63 0.3989376427616378 2227 64-65 0.3120866020767039 2227 66-67 0.4047394689087227 2227 68-69 0.3563241097001253 2227 70-71 0.49679412907442355 2227 72-73 0.41624272842804544 2227 74-75 0.39777098762777285 2227 76-77 0.6639144441225895 2227 78-79 0.44793356513818594 2227 80-81 0.3301942608635642 2227 82-83 0.22051454001685755 2227 84-85 0.24396299332629923 2227 86-87 0.33656706636826783 2227 88-89 0.3581305377461632 2227 90-91 0.31006011382514487 2227 92-93 0.4233150700350663 2227 94-95 0.5058099753685852 2227 96-97 0.47447516332349693 2227 98-99 0.5607564455150893 2227 100 0.41428496209805843 2228 1 1.4043386045408024 2228 2 1.5738925799523589 2228 3 -0.18564850107855335 2228 4 -0.03276234101854669 2228 5 3.187305215524262 2228 6 0.4351616536520666 2228 7 0.4620115895095367 2228 8 0.36788442557618595 2228 9 0.2801460537097711 2228 10-11 0.3066739687679032 2228 12-13 0.25988514184511047 2228 14-15 0.1653979458424928 2228 16-17 0.26171737648459725 2228 18-19 0.26141721506161275 2228 20-21 0.25468267029840064 2228 22-23 0.3323035918069266 2228 24-25 0.3650930977892486 2228 26-27 0.35403949304414795 2228 28-29 0.34900979026183876 2228 30-31 0.2928703770595291 2228 32-33 0.218823918236005 2228 34-35 0.28593348341196645 2228 36-37 0.3018380887972043 2228 38-39 0.31173329997518096 2228 40-41 0.2888522758443699 2228 42-43 0.3477907073552444 2228 44-45 0.36637436929294864 2228 46-47 0.448816027542442 2228 48-49 0.35681019011531845 2228 50-51 0.2679743483155761 2228 52-53 0.38301077250481086 2228 54-55 0.3882474525320063 2228 56-57 0.5842370889366393 2228 58-59 0.5928950329637601 2228 60-61 0.39480893065072564 2228 62-63 0.46763995641808265 2228 64-65 0.4566885852107987 2228 66-67 0.5760890503500562 2228 68-69 0.4609869460143443 2228 70-71 0.5211462126008186 2228 72-73 0.5505877072684342 2228 74-75 0.5406385006787744 2228 76-77 0.5526581502974892 2228 78-79 0.5868085535544552 2228 80-81 0.5888712447699049 2228 82-83 0.5002059495247764 2228 84-85 0.48694016566127374 2228 86-87 0.5255594365532943 2228 88-89 0.5331652889277052 2228 90-91 0.6126625911982728 2228 92-93 0.567904697193498 2228 94-95 0.848549295172127 2228 96-97 0.4161688586869161 2228 98-99 0.6718057767479095 2228 100 0.6986052289871338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 20.0 13 43.0 14 166.0 15 551.0 16 2025.0 17 5186.0 18 8542.0 19 10925.0 20 12744.0 21 14659.0 22 16973.0 23 19747.0 24 22472.0 25 25527.0 26 28768.0 27 32432.0 28 36969.0 29 42061.0 30 48427.0 31 55887.0 32 66112.0 33 78742.0 34 95818.0 35 123547.0 36 174464.0 37 285863.0 38 704929.0 39 1379422.0 40 1850.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.87821801599427 18.149878935536467 20.24410758562234 35.72779546284692 2 17.895852518337172 50.27295568544869 15.66394732832444 16.16724446788969 3 41.96652919036891 15.561367956438962 22.849331582329018 19.622771270863108 4 16.905123144426597 16.52040398546711 21.91424186257975 44.66023100752655 5 17.273180042557055 35.30329411986084 26.46652934211992 20.956996495462192 6 17.494979317854934 28.683186686216246 30.246070331736814 23.575763664192014 7 20.275300611162013 26.366009473512015 36.03609974411745 17.322590171208525 8 21.142436228914576 28.033783416710396 33.6560672633314 17.167713091043623 9 20.89371632455413 28.313005273954577 27.60854673824863 23.18473166324266 10-11 19.78124484995012 30.306710035081796 28.704993306262978 21.207051808705106 12-13 24.233376663304877 26.75124458590336 27.327534219093856 21.687844531697902 14-15 21.813646118467155 27.492442040978236 28.688027543415206 22.005884297139403 16-17 20.62634622114189 29.684773698272256 28.39695393233908 21.291926148246777 18-19 21.30822420665331 29.23144790797576 29.92756013816626 19.532767747204673 20-21 22.79841911868477 28.651379674651906 29.94604341110775 18.604157795555576 22-23 21.621711441813655 30.346650900748468 28.768410053079467 19.26322760435841 24-25 22.194571502192346 28.440916199754103 26.6971453510623 22.667366946991248 26-27 20.20332510741695 26.82620958453305 29.875555067254528 23.094910240795468 28-29 19.73752179149124 28.09997620539118 30.654967852997878 21.5075341501197 30-31 20.36722825154915 29.757963542765392 28.423317939199492 21.451490266485965 32-33 20.72263223658832 30.015515023198176 28.807865315516146 20.453987424697356 34-35 21.314127320917876 29.19803233571208 30.031281953071908 19.456558390298134 36-37 21.738756995342154 28.062722334197606 28.913802695158626 21.28471797530161 38-39 21.40442226572109 29.29098886003746 29.041265769498732 20.26332310474272 40-41 21.053399129363008 29.578257323824698 29.90924566950212 19.45909787731017 42-43 20.472716964685684 29.705787910168173 29.368885187509786 20.45260993763635 44-45 20.486553191308552 30.394361418094974 28.90855211023436 20.210533280362114 46-47 19.90354188633112 28.26909267275262 28.879812965772107 22.94755247514415 48-49 20.973194693569095 28.03195573725215 30.16263160610282 20.832217963075934 50-51 21.87448309812433 30.40357270436384 28.27327685970103 19.4486673378108 52-53 20.5160423469946 30.280532222258138 28.237526315877332 20.96589911486993 54-55 22.206163838918933 28.471786801739224 28.213289329113227 21.108760030228616 56-57 21.414881892082136 28.608809201553992 29.464966776314494 20.511342130049382 58-59 21.183959062602067 28.042636647203313 29.15850128521283 21.61490300498179 60-61 19.73833505950552 30.325241189882735 29.311238329368827 20.625185421242918 62-63 20.634948579764615 31.061537487879313 27.61389801065209 20.689615921703982 64-65 19.747464883651656 30.337750960319056 28.712769568800695 21.202014587228597 66-67 20.058069041246227 30.262155938730228 29.41465690159038 20.265118118433165 68-69 20.87524822671358 30.01864109399124 29.192614824677506 19.913495854617675 70-71 19.690467396143884 30.841996545540024 29.14249146628199 20.325044592034104 72-73 20.061695890370217 30.66950117318705 28.57392595946852 20.694876976974214 74-75 19.987535172090197 29.405733942239316 30.29909213441133 20.307638751259155 76-77 19.497379411819157 30.754815590655905 28.712535331428757 21.035269666096184 78-79 19.70583977339325 29.35890358074717 28.55952478867913 22.375731857180448 80-81 20.53444702640944 29.614990120228047 28.54128858448916 21.309274268873356 82-83 20.656863349126567 28.299636010028923 29.59686433854315 21.446636302301364 84-85 20.88731243195864 28.980315162566306 28.21617295487441 21.916199450600647 86-87 21.36238414189812 28.305053521063495 29.661343342425244 20.67121899461314 88-89 21.109961512909003 27.526813644600956 29.970232522001623 21.392992320488418 90-91 21.08530197388608 28.489901425579333 29.232661916050734 21.192134684483854 92-93 21.2116195140872 29.16353933128186 29.722711450615215 19.902129704015724 94-95 20.822681677067177 29.892596705606987 29.154221819306432 20.130499798019407 96-97 20.94201867083719 29.203616772856968 29.41527908072881 20.43908547557704 98-99 21.927504900798848 29.718219620737806 28.66638784947878 19.687887628984566 100 21.445816846850757 30.6378914379349 27.62636230674888 20.28992940846546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 10.5 2 20.5 3 20.0 4 24.5 5 25.5 6 31.0 7 47.0 8 52.0 9 67.0 10 80.0 11 93.0 12 117.0 13 128.5 14 146.5 15 196.0 16 268.0 17 373.0 18 552.5 19 888.5 20 1409.0 21 2208.0 22 3512.5 23 5350.5 24 8042.5 25 11899.0 26 16976.5 27 22982.5 28 29913.0 29 37987.0 30 47363.5 31 58378.0 32 70598.5 33 84076.5 34 98523.0 35 114220.5 36 130807.5 37 143583.0 38 151849.5 39 157934.5 40 160990.0 41 161147.0 42 159636.0 43 157127.0 44 154326.0 45 149364.0 46 139178.0 47 127681.0 48 119516.0 49 114329.0 50 109017.0 51 100395.5 52 87900.5 53 73523.5 54 61395.0 55 51279.0 56 40838.5 57 30421.5 58 21665.5 59 15654.5 60 11947.0 61 9795.0 62 7859.0 63 5839.0 64 4248.0 65 3251.0 66 2796.5 67 2399.0 68 1965.0 69 1793.5 70 1814.5 71 1564.5 72 1025.0 73 607.0 74 369.0 75 233.5 76 160.5 77 116.0 78 103.0 79 82.0 80 55.5 81 43.0 82 40.0 83 37.5 84 26.5 85 22.5 86 22.0 87 18.0 88 20.5 89 19.5 90 16.5 91 16.0 92 15.5 93 19.0 94 20.0 95 19.0 96 16.5 97 15.0 98 42.0 99 106.0 100 121.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.341870440451235 2 0.0059789897692504495 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.1245141312057437E-4 30-31 0.009894165811043892 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.52529340298907E-4 40-41 0.0012747084787234461 42-43 9.10506056231033E-5 44-45 0.0 46-47 0.002215564736828847 48-49 3.0350201874367767E-5 50-51 0.0 52-53 0.0013505839834093657 54-55 0.019469654502406925 56-57 0.011654477519757223 58-59 0.01441634589032469 60-61 2.57976715932126E-4 62-63 0.012124905648809924 64-65 7.587550468591941E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2762651405775828E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3294871.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.17742862161354 #Duplication Level Percentage of deduplicated Percentage of total 1 85.37494585701086 62.475190065255326 2 8.97146765311339 13.130178676336396 3 2.5520625659553113 5.602601287742601 4 1.09179222698465 3.195781910392069 5 0.5878150513192162 2.150739698031102 6 0.35504655630085036 1.5588836418633103 7 0.23620007911250057 1.209916010077214 8 0.1622097403628667 0.9496073357707314 9 0.12029956090164351 0.7922891277982322 >10 0.5156694126433039 6.328016095146595 >50 0.022333792634432092 1.1111550739817573 >100 0.009693514981708859 1.249848615921674 >500 4.2180789058480437E-4 0.20705460686000124 >1k 4.2180788766919095E-5 0.03873785482289199 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.0350201874367767E-5 0.0 0.0 0.0 0.0 2 3.0350201874367767E-5 0.0 0.0 3.0350201874367767E-5 0.0 3 3.0350201874367767E-5 0.0 0.0 3.0350201874367767E-5 0.0 4 3.0350201874367767E-5 0.0 0.0 3.0350201874367767E-5 0.0 5 3.0350201874367767E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 6 3.0350201874367767E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 7 3.0350201874367767E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 8 6.070040374873553E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 9 6.070040374873553E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 10-11 6.070040374873553E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 12-13 6.070040374873553E-5 3.0350201874367767E-5 0.0 3.0350201874367767E-5 0.0 14-15 6.070040374873553E-5 3.0350201874367767E-5 0.0 9.10506056231033E-5 0.0 16-17 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 18-19 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 20-21 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 22-23 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 24-25 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 26-27 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 28-29 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 30-31 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 32-33 6.070040374873553E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 34-35 7.587550468591943E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 36-37 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 38-39 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 40-41 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 42-43 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 44-45 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 46-47 9.10506056231033E-5 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 48-49 1.5175100937183883E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 50-51 1.5175100937183883E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 52-53 1.5175100937183883E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 54-55 1.972763121833905E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 56-57 2.4280161499494213E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 58-59 2.4280161499494213E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 60-61 2.883269178064938E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 62-63 3.3385222061804547E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 64-65 3.3385222061804547E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 66-67 3.94552624366781E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 68-69 5.007783309270682E-4 3.0350201874367767E-5 0.0 1.2140080749747107E-4 0.0 70-71 5.766538356129875E-4 4.552530281155165E-5 0.0 1.2140080749747107E-4 0.0 72-73 6.221791384245392E-4 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 74-75 7.132297440476426E-4 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 76-77 8.194554506079297E-4 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 78-79 9.408562581054009E-4 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 80-81 0.0013505839834093657 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 82-83 0.0015175100937183884 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 84-85 0.001547860295592756 6.070040374873553E-5 0.0 1.2140080749747107E-4 0.0 86-87 0.001578210497467124 1.5175100937183883E-4 0.0 1.2140080749747107E-4 0.0 88 0.001578210497467124 1.5175100937183883E-4 0.0 1.2140080749747107E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 12550 0.0 81.27339 8 CACCGGC 11955 0.0 79.97861 9 CTTCACC 14280 0.0 74.31984 6 CAGCTGC 100 0.0 74.10087 1 CCTTCAC 15700 0.0 71.514465 5 TTCACCG 15495 0.0 67.67433 7 GTGTGGC 4825 0.0 66.73585 7 GGCCCGA 895 0.0 65.032585 9 CGTAGTG 2800 0.0 63.1998 4 CGGGGCA 1560 0.0 61.381565 94 ATGCAGC 70 2.1827873E-11 60.352695 2 ACCTTCA 6455 0.0 60.209705 4 CGGGCCC 995 0.0 59.91189 7 TGTGGCA 5695 0.0 59.095963 8 AACGGGC 1010 0.0 59.02211 5 GGGCCCG 1030 0.0 57.420338 8 GCGTAGT 1895 0.0 57.218327 3 GCCTTCA 4690 0.0 56.246544 4 ATCGGAA 5030 0.0 55.05749 94 GTCCTTC 4355 0.0 53.56741 3 >>END_MODULE