FastQCFastQC Report
Tue 7 Mar 2017
1SN_4A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_4A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3294871
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG125500.081.273398
CACCGGC119550.079.978619
CTTCACC142800.074.319846
CAGCTGC1000.074.100871
CCTTCAC157000.071.5144655
TTCACCG154950.067.674337
GTGTGGC48250.066.735857
GGCCCGA8950.065.0325859
CGTAGTG28000.063.19984
CGGGGCA15600.061.38156594
ATGCAGC702.1827873E-1160.3526952
ACCTTCA64550.060.2097054
CGGGCCC9950.059.911897
TGTGGCA56950.059.0959638
AACGGGC10100.059.022115
GGGCCCG10300.057.4203388
GCGTAGT18950.057.2183273
GCCTTCA46900.056.2465444
ATCGGAA50300.055.0574994
GTCCTTC43550.053.567413
Produced by FastQC (version 0.11.5)