FastQCFastQC Report
Tue 7 Mar 2017
1SN_5A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_5A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3000852
Sequences flagged as poor quality0
Sequence length94
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA143560.47839746845229286No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG82730.2756883711692546No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG59740.19907679552340468No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT59310.19764386914116389No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA56440.18807991863644058No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG51480.17155127943664No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG49670.16551965908348695No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA48150.16045443094161257No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT43210.14399243948052087No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC39940.13309553420162007No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA39360.13116274977906273No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA39090.13026300530649296No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA38810.12932993696456874No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG37260.12416473721463106No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC36440.12143217992756723No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT33990.11326783193573026No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA32070.10686964901967841No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30920.10303740404391819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC3600.087.9998861
GCTGCCC900.087.9998863
GCAGCTC158.971912E-487.9998863
GCAGCCC302.0971129E-887.9998863
GCAGCCA356.0754246E-1087.9998863
GCAGCAC202.5504422E-587.9998863
TGCAGCC158.971912E-487.9998862
AGCAGCT450.087.9998862
AGCAGCC600.087.9998862
AGCAGCA1650.085.333222
CAGCTGC4750.083.368321
CTGCTGC1900.083.368311
GCAGCAA950.083.368313
GCTGCAA600.080.666563
CAGCCCT157400.080.396331
CAGCGTA65850.080.115311
AGCAGCG1050.079.618942
AGCTGCT1450.078.896442
AGCTGCA1450.078.896442
AGCTGCG950.078.736742
Produced by FastQC (version 0.11.5)