##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_5A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3000852
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.8323542780517	12.0	12.0	12.0	2.0	32.0
2	14.658421674910992	12.0	12.0	12.0	12.0	22.0
3	13.697844478834678	12.0	12.0	12.0	12.0	22.0
4	13.8511542721867	12.0	12.0	12.0	12.0	22.0
5	22.442017800278055	22.0	22.0	22.0	22.0	27.0
6	26.897982306358326	27.0	22.0	32.0	22.0	32.0
7	34.99807587978348	37.0	37.0	37.0	27.0	41.0
8	37.61342378764431	41.0	37.0	41.0	27.0	41.0
9	38.55393968113056	41.0	41.0	41.0	32.0	41.0
10-11	38.903786991161176	41.0	41.0	41.0	37.0	41.0
12-13	39.08545939619815	41.0	41.0	41.0	37.0	41.0
14-15	39.0425092607033	41.0	41.0	41.0	37.0	41.0
16-17	38.95134931679403	41.0	41.0	41.0	37.0	41.0
18-19	38.89351090956835	41.0	41.0	41.0	37.0	41.0
20-21	38.90979861719272	41.0	41.0	41.0	37.0	41.0
22-23	38.89051109484906	41.0	41.0	41.0	37.0	41.0
24-25	38.913004040185925	41.0	41.0	41.0	37.0	41.0
26-27	38.87869178486643	41.0	41.0	41.0	37.0	41.0
28-29	38.82990347408003	41.0	41.0	41.0	37.0	41.0
30-31	38.70214908965853	41.0	41.0	41.0	32.0	41.0
32-33	38.74792775518419	41.0	41.0	41.0	32.0	41.0
34-35	38.722486480506205	41.0	41.0	41.0	32.0	41.0
36-37	38.64934075389256	41.0	41.0	41.0	32.0	41.0
38-39	38.55183644511625	41.0	41.0	41.0	32.0	41.0
40-41	38.603347316028916	41.0	41.0	41.0	32.0	41.0
42-43	38.34405028971772	41.0	41.0	41.0	32.0	41.0
44-45	38.44848279755216	41.0	41.0	41.0	32.0	41.0
46-47	38.45937120524438	41.0	41.0	41.0	32.0	41.0
48-49	38.40597853542927	41.0	41.0	41.0	32.0	41.0
50-51	38.29983184775524	41.0	41.0	41.0	32.0	41.0
52-53	38.26490976562656	41.0	41.0	41.0	32.0	41.0
54-55	38.134959338214614	41.0	39.0	41.0	32.0	41.0
56-57	37.923089675865384	41.0	37.0	41.0	29.5	41.0
58-59	37.92345607180894	41.0	37.0	41.0	29.5	41.0
60-61	37.924405635466194	41.0	37.0	41.0	29.5	41.0
62-63	37.917366801161805	41.0	37.0	41.0	32.0	41.0
64-65	37.89226792924143	41.0	37.0	41.0	29.5	41.0
66-67	37.77701099554393	41.0	37.0	41.0	27.0	41.0
68-69	37.7939955052765	41.0	37.0	41.0	27.0	41.0
70-71	37.77306211702543	41.0	37.0	41.0	27.0	41.0
72-73	37.632489706256756	41.0	37.0	41.0	27.0	41.0
74-75	37.5355965572444	41.0	37.0	41.0	27.0	41.0
76-77	36.49770381878213	39.0	34.5	41.0	24.5	41.0
78-79	37.01322174502441	41.0	37.0	41.0	27.0	41.0
80-81	37.6620061569181	41.0	37.0	41.0	27.0	41.0
82-83	37.854416179138454	41.0	37.0	41.0	32.0	41.0
84-85	37.760132622335256	41.0	37.0	41.0	27.0	41.0
86-87	37.61439251252644	41.0	37.0	41.0	27.0	41.0
88-89	37.54072859974434	41.0	37.0	41.0	27.0	41.0
90-91	37.389359255304825	41.0	37.0	41.0	27.0	41.0
92-93	37.28544593335493	41.0	37.0	41.0	27.0	41.0
94-95	37.11433086336814	41.0	37.0	41.0	27.0	41.0
96-97	37.052646881618955	41.0	37.0	41.0	27.0	41.0
98-99	36.93750174950314	41.0	37.0	41.0	27.0	41.0
100	36.79582165331713	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.3599717197630845
1101	2	-0.5253133912855876
1101	3	-0.021318162140918417
1101	4	0.0067574113538437786
1101	5	-1.08676933072093
1101	6	-0.910228894573013
1101	7	-0.35743245836334836
1101	8	-0.18311977211597252
1101	9	-0.04285347586727539
1101	10-11	-0.10869404267809557
1101	12-13	-0.06640953007106987
1101	14-15	0.13755960292011338
1101	16-17	-0.044627294010091134
1101	18-19	0.09180317978596975
1101	20-21	0.08458912600316637
1101	22-23	0.17142400247296052
1101	24-25	0.15219200496633079
1101	26-27	0.09357476374738383
1101	28-29	-0.0763356681280527
1101	30-31	-0.02873129498728133
1101	32-33	-0.03975296459350375
1101	34-35	0.030946015634619073
1101	36-37	0.060215488911893544
1101	38-39	0.036252030006579616
1101	40-41	0.040157896701700224
1101	42-43	0.09248896489382474
1101	44-45	-0.0287259228337291
1101	46-47	-0.04201773045642909
1101	48-49	-0.06033067569980943
1101	50-51	0.062160205301879046
1101	52-53	-0.011601162189478487
1101	54-55	-0.0977692305907425
1101	56-57	0.025588130618515947
1101	58-59	0.02236703394798667
1101	60-61	-0.09031739030260866
1101	62-63	-0.16293722692854828
1101	64-65	0.06429636893518875
1101	66-67	-0.07006460535153991
1101	68-69	-0.07908384861995188
1101	70-71	-0.06263360437997534
1101	72-73	0.0456218022913788
1101	74-75	0.16018057950379472
1101	76-77	-0.2715902422495944
1101	78-79	-0.14536216554269998
1101	80-81	-0.11496896647441446
1101	82-83	-0.1471546283844063
1101	84-85	-0.06869163705829351
1101	86-87	-0.01723547172633033
1101	88-89	-0.40019724357203756
1101	90-91	-0.23816378557091866
1101	92-93	0.0381379522107963
1101	94-95	0.12198093679579358
1101	96-97	-0.061162835802406335
1101	98-99	-0.4203634034928285
1101	100	-0.6328146183413921
1102	1	1.9177677244955191
1102	2	0.7739676425695272
1102	3	-0.0388770900622859
1102	4	0.033155527480527525
1102	5	-0.84715566126334
1102	6	-0.3403310514714448
1102	7	-0.35281150823593777
1102	8	-0.37711208190847145
1102	9	-0.4886591274872245
1102	10-11	-0.5667164307377988
1102	12-13	-0.5963615231544281
1102	14-15	-0.40827221360701316
1102	16-17	-0.34449021264860136
1102	18-19	-0.33563186298796666
1102	20-21	-0.40642891640673184
1102	22-23	-0.4637592656377052
1102	24-25	-0.4209872587759378
1102	26-27	-0.40593151583033915
1102	28-29	-0.34602191676292904
1102	30-31	-0.43434025476158666
1102	32-33	-0.5111461890201525
1102	34-35	-0.3582302448313541
1102	36-37	-0.4867704275424174
1102	38-39	-0.4577442614022331
1102	40-41	-0.3942768275429387
1102	42-43	-0.3236422691797074
1102	44-45	-0.26893399072597646
1102	46-47	-0.5126584294007301
1102	48-49	-0.5446687035483677
1102	50-51	-0.47626864890271037
1102	52-53	-0.4777870012866714
1102	54-55	-0.5195668734738916
1102	56-57	-0.4206765444815943
1102	58-59	-0.4398570969220543
1102	60-61	-0.2828820062472772
1102	62-63	-0.49087224695038856
1102	64-65	-0.5783495857426431
1102	66-67	-0.35071793170755683
1102	68-69	-0.5844977091951193
1102	70-71	-0.6125448712165209
1102	72-73	-0.43167672792340284
1102	74-75	-0.5579772972708739
1102	76-77	-0.395171080436711
1102	78-79	-0.4828490830891212
1102	80-81	-0.5228406219167212
1102	82-83	-0.3253330958034084
1102	84-85	-0.42672198835060726
1102	86-87	-0.3927786779513056
1102	88-89	-0.6253229488869181
1102	90-91	-0.7424423166957794
1102	92-93	-0.47666171560864967
1102	94-95	-0.2705034780203661
1102	96-97	-0.47015730704376324
1102	98-99	-0.4733314140498024
1102	100	-0.7419268316650118
1103	1	1.402581671164823
1103	2	0.7508103353881133
1103	3	0.3060780260951912
1103	4	1.1363196234682427
1103	5	2.8340833871497395
1103	6	-0.06147671111025588
1103	7	-0.6138717278329224
1103	8	-0.06789505560582398
1103	9	-0.3718570674112911
1103	10-11	-0.18454667407499414
1103	12-13	-0.44711744760967065
1103	14-15	-0.38510430811567176
1103	16-17	-0.26150272277588016
1103	18-19	-0.3737802587571224
1103	20-21	-0.18764955025632446
1103	22-23	-0.11651881530374197
1103	24-25	-0.3003616375972129
1103	26-27	-0.36648019033504653
1103	28-29	-0.38437145962249275
1103	30-31	-0.3612502615680597
1103	32-33	-0.39395177067895304
1103	34-35	-0.39048023953219513
1103	36-37	-0.5099266307332186
1103	38-39	-0.3620775128930589
1103	40-41	-0.4435200346150694
1103	42-43	-0.15312076167609945
1103	44-45	-0.568242995638883
1103	46-47	-0.6299858418115889
1103	48-49	-0.45692389730902505
1103	50-51	-0.2899248214761627
1103	52-53	-0.20132361020432654
1103	54-55	-0.4603542631337092
1103	56-57	-0.5900443817904701
1103	58-59	-0.5381963859167698
1103	60-61	-0.37359588485188056
1103	62-63	-0.42117010656281906
1103	64-65	-0.5338804756945024
1103	66-67	-0.5859803193654756
1103	68-69	-0.4331383876282686
1103	70-71	-0.6735148874201613
1103	72-73	-0.47649617099398967
1103	74-75	-0.511135637787504
1103	76-77	-0.7196922870660742
1103	78-79	-0.6033021873340942
1103	80-81	-0.40182286327085137
1103	82-83	-0.5767376662656787
1103	84-85	-0.602494811222229
1103	86-87	-0.36423382295973994
1103	88-89	-0.396334171908336
1103	90-91	-0.6266800685822744
1103	92-93	-0.39624048872473594
1103	94-95	-0.616331556358368
1103	96-97	-0.35102472277542063
1103	98-99	-0.40072960550708814
1103	100	-0.4317734741204333
1104	1	1.7851440643183345
1104	2	-0.5893932163753846
1104	3	-0.04123260338759849
1104	4	0.11230590208404934
1104	5	-1.1288431544275106
1104	6	-0.5631113433158461
1104	7	-0.3239836709104793
1104	8	-0.12869514636579993
1104	9	-0.11387616511163401
1104	10-11	-0.2620639643700855
1104	12-13	-0.12788438672947677
1104	14-15	-0.21999065029810794
1104	16-17	-0.1226979831652173
1104	18-19	-0.23112255030795836
1104	20-21	-0.1336852532217847
1104	22-23	-0.07812812478313447
1104	24-25	-0.06745945745251447
1104	26-27	-0.11350948987241338
1104	28-29	-0.12043551642022976
1104	30-31	-0.11986726312863993
1104	32-33	-0.12690158249056793
1104	34-35	-0.22522520226690546
1104	36-37	-0.21509657186047804
1104	38-39	-0.0729260147472317
1104	40-41	-0.1459262565232109
1104	42-43	-0.12566521934194697
1104	44-45	-0.11966138007666416
1104	46-47	-0.32352694447601493
1104	48-49	-0.45819003361873456
1104	50-51	-0.22412362219739634
1104	52-53	0.02613448691923992
1104	54-55	-0.2436505303698766
1104	56-57	-0.15610625619182628
1104	58-59	-0.26248979178880205
1104	60-61	-0.14924835208562826
1104	62-63	-0.09904926762194322
1104	64-65	-0.23681411005996722
1104	66-67	-0.4684853380111136
1104	68-69	-0.5612188574323866
1104	70-71	-0.3405528645812126
1104	72-73	-0.15331401191114935
1104	74-75	-0.278986254487684
1104	76-77	-0.3919664999744228
1104	78-79	-0.21639210552887533
1104	80-81	-0.23581178154703508
1104	82-83	-0.23673503353354164
1104	84-85	-0.22912489676870962
1104	86-87	-0.2869323907188104
1104	88-89	-0.303417130683556
1104	90-91	-0.2614989037022326
1104	92-93	-0.2616654411363726
1104	94-95	-0.18298861008862133
1104	96-97	-0.19553720833437183
1104	98-99	-0.41324958915643606
1104	100	-0.3691564517751189
1105	1	1.4841741539199838
1105	2	0.30830804953291846
1105	3	-0.0808545199001447
1105	4	-0.19228269882970217
1105	5	-1.0721496326013593
1105	6	0.4793345874518913
1105	7	0.1825603740845807
1105	8	-0.04630174361441419
1105	9	-0.03827889116367089
1105	10-11	-0.17921643073779592
1105	12-13	-0.15173607531096422
1105	14-15	-0.14950366829443595
1105	16-17	-0.11660743567465204
1105	18-19	-0.28161785065505285
1105	20-21	-0.24220522146850954
1105	22-23	-0.08309928504425557
1105	24-25	-0.1393977159207651
1105	26-27	-0.16931945161647377
1105	28-29	-0.07758612488382255
1105	30-31	-0.2165369700020463
1105	32-33	-0.32113173458535016
1105	34-35	-0.21381429299174926
1105	36-37	-0.18111001633025836
1105	38-39	-0.152214184120524
1105	40-41	-0.03540988585444893
1105	42-43	0.0856137365159455
1105	44-45	-0.14309335122847955
1105	46-47	-0.24785260973236234
1105	48-49	-0.1666198512907755
1105	50-51	-0.2882059020146457
1105	52-53	-0.16654325961197003
1105	54-55	-0.26810434878508715
1105	56-57	-0.0619217071980529
1105	58-59	-0.11896268750126637
1105	60-61	-0.2042045224385376
1105	62-63	-0.3359970689613192
1105	64-65	-0.4642150288780016
1105	66-67	-0.42311046363075633
1105	68-69	-0.47567820733484467
1105	70-71	-0.3157690570600735
1105	72-73	-0.23948233802398988
1105	74-75	-0.3317460866007096
1105	76-77	-0.38121092415082813
1105	78-79	-0.30867694756479835
1105	80-81	-0.33011814993563604
1105	82-83	-0.41894644613182663
1105	84-85	-0.1623813804318175
1105	86-87	-0.18566521566202
1105	88-89	-0.27984982810698256
1105	90-91	-0.30503001937365326
1105	92-93	-0.3373184522077892
1105	94-95	-0.06572741410506922
1105	96-97	-0.1907433934660716
1105	98-99	-0.4089778875696197
1105	100	-0.28651223196816744
1106	1	1.3000430292596796
1106	2	-0.7395861626547404
1106	3	0.032858795803763385
1106	4	-0.25264273026157547
1106	5	-1.0706849691890312
1106	6	-0.5606155492711302
1106	7	-0.33468994195770563
1106	8	-0.13596615841384363
1106	9	-0.1383120254800616
1106	10-11	-0.06916786891486026
1106	12-13	-0.1618540714656973
1106	14-15	-0.08917867059836482
1106	16-17	-0.14859175913311162
1106	18-19	-0.06494390288064267
1106	20-21	-0.29515561777885324
1106	22-23	-0.1748035355931492
1106	24-25	-0.30422954343659114
1106	26-27	-0.24957730835382108
1106	28-29	-0.2787108625704846
1106	30-31	-0.1393158628093687
1106	32-33	-0.3774097730056809
1106	34-35	-0.297690754753269
1106	36-37	-0.4320991565756529
1106	38-39	-0.10915153212300055
1106	40-41	-0.20511513113632418
1106	42-43	-0.14890455313426543
1106	44-45	-0.17712243485710388
1106	46-47	-0.3727009551621876
1106	48-49	-0.3180832835979359
1106	50-51	-0.4451041360470356
1106	52-53	-0.19455392731417476
1106	54-55	-0.20195629396643255
1106	56-57	-0.2787303730528947
1106	58-59	-0.35929439647926387
1106	60-61	-0.21901535081755696
1106	62-63	-0.40809108037331754
1106	64-65	-0.43503263044594576
1106	66-67	-0.44095754911237606
1106	68-69	-0.39114771215620436
1106	70-71	-0.6559877154086919
1106	72-73	-0.46857848874503105
1106	74-75	-0.5706404275268042
1106	76-77	-0.5742966857511789
1106	78-79	-0.43409286765411537
1106	80-81	-0.4741047639532141
1106	82-83	-0.4894829336269666
1106	84-85	-0.6758972972147319
1106	86-87	-0.5283605058757175
1106	88-89	-0.655571660244739
1106	90-91	-0.8052476787683531
1106	92-93	-0.5616305985728332
1106	94-95	-0.39308214897074123
1106	96-97	-0.5068349933188969
1106	98-99	-0.6934200245712248
1106	100	-0.6083798255515092
1107	1	1.4495070491584041
1107	2	-0.5675418261505367
1107	3	-0.11434246660049219
1107	4	-0.18788681464606682
1107	5	-1.2475383707173258
1107	6	-0.3395746131102335
1107	7	-0.5700186963576712
1107	8	-0.4425146406734868
1107	9	-0.4032967278851345
1107	10-11	-0.2763085757529069
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2221	4	-0.3248742222224177
2221	5	-0.7164359381819523
2221	6	-0.39209753574794703
2221	7	0.11871289334593627
2221	8	0.12677585018161608
2221	9	0.1749329175653358
2221	10-11	0.0765291996080606
2221	12-13	0.19949419322395556
2221	14-15	0.14720448005914477
2221	16-17	0.19261280245810752
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2221	20-21	0.04320944482556399
2221	22-23	0.13797415427993087
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2221	26-27	0.17570726320620622
2221	28-29	0.04165436034615766
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2221	32-33	0.1025757183214111
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2221	38-39	0.0521449297695753
2221	40-41	0.24331434391893936
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2221	50-51	0.2839303523353962
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2221	60-61	0.25258827012711293
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2221	64-65	0.2606932428771245
2221	66-67	0.22656657030606908
2221	68-69	0.45203734441081167
2221	70-71	0.5172201946797657
2221	72-73	0.39911475034397625
2221	74-75	0.3030677150421681
2221	76-77	0.7507360678140671
2221	78-79	0.46594176478705407
2221	80-81	0.28805059081536655
2221	82-83	0.312584538370146
2221	84-85	0.3654729805380015
2221	86-87	0.367980277345346
2221	88-89	0.46458964385768553
2221	90-91	0.39673794230883885
2221	92-93	0.23514101332767012
2221	94-95	0.42617269648008005
2221	96-97	0.28550622221727195
2221	98-99	0.3194721204493831
2221	100	0.5292397435420142
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2222	2	1.6512619557867865
2222	3	0.6296443086444725
2222	4	0.8391468161884763
2222	5	3.2744752817406066
2222	6	0.4902660023281378
2222	7	0.6676006094930145
2222	8	0.5776655334254173
2222	9	0.2876981408146193
2222	10-11	0.325252213648227
2222	12-13	0.37049300662584983
2222	14-15	0.33863206636331
2222	16-17	0.28703889158465046
2222	18-19	0.316123747432151
2222	20-21	0.18093365663577288
2222	22-23	0.2594238735643728
2222	24-25	0.26725168123762444
2222	26-27	0.16542259836326423
2222	28-29	0.17083009431021878
2222	30-31	0.13279166195163583
2222	32-33	0.27168480057987665
2222	34-35	0.24280847857339438
2222	36-37	0.26646818618407764
2222	38-39	0.3828322391747818
2222	40-41	0.244172138298822
2222	42-43	0.4523490710472444
2222	44-45	0.3928065761434141
2222	46-47	0.2822953408748532
2222	48-49	0.4631937412690448
2222	50-51	0.28989923265852724
2222	52-53	0.41129314397403505
2222	54-55	0.2878924207843738
2222	56-57	0.3104509690736279
2222	58-59	0.38016775527562885
2222	60-61	0.4382324300660727
2222	62-63	0.4165211888408962
2222	64-65	0.4322443683298758
2222	66-67	0.5666274740561548
2222	68-69	0.4873557100642927
2222	70-71	0.540201263712909
2222	72-73	0.4452661827174538
2222	74-75	0.5069156891601665
2222	76-77	0.684741271417515
2222	78-79	0.6524416243699207
2222	80-81	0.3878585454216008
2222	82-83	0.2269824546943795
2222	84-85	0.36085869614240096
2222	86-87	0.4597592486260922
2222	88-89	0.3356477522524486
2222	90-91	0.36260966598467803
2222	92-93	0.46442822914007564
2222	94-95	0.5314181667182751
2222	96-97	0.607682549550205
2222	98-99	0.5263716361045283
2222	100	0.6643443483721683
2223	1	1.4753822362414653
2223	2	-0.8787465019752325
2223	3	0.09333990113386825
2223	4	-0.04486981164717285
2223	5	-1.0913149726768978
2223	6	0.07024717810228509
2223	7	0.28678361596116275
2223	8	0.1858111258802353
2223	9	0.03559818846300544
2223	10-11	0.025755944952805976
2223	12-13	0.021457419710415593
2223	14-15	0.0649681403434883
2223	16-17	0.09354957028720179
2223	18-19	0.020645157741491005
2223	20-21	0.0235926580666046
2223	22-23	0.11037908891918136
2223	24-25	0.028302131131084707
2223	26-27	0.10181704680721992
2223	28-29	0.07756781171032401
2223	30-31	0.02083544314525909
2223	32-33	0.17022305310804597
2223	34-35	0.2014337015146097
2223	36-37	0.030125950416525882
2223	38-39	0.003665996087775625
2223	40-41	0.06659820895259827
2223	42-43	0.24477449855150013
2223	44-45	0.1954006817125986
2223	46-47	0.08074037129998146
2223	48-49	0.14017645714426408
2223	50-51	0.10164853712343813
2223	52-53	0.047485311519324114
2223	54-55	0.0027595481673969857
2223	56-57	0.1159653192609369
2223	58-59	0.21147295462086646
2223	60-61	0.13716639244113082
2223	62-63	0.09491302457635697
2223	64-65	0.10495083142934902
2223	66-67	0.10867257889298543
2223	68-69	0.04667719423209604
2223	70-71	0.18694447208851273
2223	72-73	0.25200562901412127
2223	74-75	0.24916452716878013
2223	76-77	0.40938952741039003
2223	78-79	0.3541004660507099
2223	80-81	0.20617634289036602
2223	82-83	0.06947527458692804
2223	84-85	0.07184050884204396
2223	86-87	0.2737234079086406
2223	88-89	0.21850207750215134
2223	90-91	0.17956799750266583
2223	92-93	0.30177384732088086
2223	94-95	0.33399270117829616
2223	96-97	0.35550417445019633
2223	98-99	0.335872337690752
2223	100	0.4814373263550209
2224	1	1.2494368755204857
2224	2	-0.9461615140396162
2224	3	-0.12544027682598546
2224	4	-0.40577372910339804
2224	5	-1.0357623640770477
2224	6	0.4323833764206171
2224	7	0.36355620981716186
2224	8	0.08738167191938828
2224	9	0.09318678075214137
2224	10-11	0.15180277162556877
2224	12-13	0.1276624843031513
2224	14-15	0.10640426439854167
2224	16-17	0.2694126429373753
2224	18-19	0.24543862148441775
2224	20-21	0.24234971375862813
2224	22-23	0.26064499499925375
2224	24-25	0.2380528594825222
2224	26-27	0.22452093760482938
2224	28-29	0.043338762525181096
2224	30-31	0.191969944017103
2224	32-33	0.21798998608882414
2224	34-35	0.30258532646598013
2224	36-37	0.1066133923048298
2224	38-39	0.0732251224468925
2224	40-41	0.13533085025005676
2224	42-43	0.276190655848211
2224	44-45	0.2723830081391725
2224	46-47	0.1515681620897027
2224	48-49	0.16795643380437042
2224	50-51	0.11613681413363963
2224	52-53	0.21130719116928276
2224	54-55	0.2843018578371286
2224	56-57	0.4042806172253606
2224	58-59	0.4043604090223951
2224	60-61	0.27005734238549906
2224	62-63	0.40657929680433824
2224	64-65	0.28084049468751715
2224	66-67	0.19127851288299524
2224	68-69	0.3470066185420322
2224	70-71	0.5187857390799806
2224	72-73	0.32902862342940864
2224	74-75	0.31342028937394417
2224	76-77	0.5051409467095951
2224	78-79	0.21857167338672667
2224	80-81	0.2013632388211164
2224	82-83	0.2847213499219947
2224	84-85	0.18527284628569873
2224	86-87	0.29441987781840595
2224	88-89	0.287991429017751
2224	90-91	0.4575944983418694
2224	92-93	0.22920780604354718
2224	94-95	0.44073631059151097
2224	96-97	0.21435442648291314
2224	98-99	0.3624076562748044
2224	100	0.46177741411082707
2225	1	1.5257869913916426
2225	2	1.9303474438075092
2225	3	0.7968253739650315
2225	4	0.9542001937477469
2225	5	3.295380457831687
2225	6	0.1306893439352237
2225	7	0.4102308413185156
2225	8	0.43377272329393435
2225	9	0.2445062713883175
2225	10-11	0.3801112382684835
2225	12-13	0.3266622600364002
2225	14-15	0.3131433575860143
2225	16-17	0.2630254057346306
2225	18-19	0.2874099262531118
2225	20-21	0.4163919010088222
2225	22-23	0.407168396498399
2225	24-25	0.34209387628943944
2225	26-27	0.2951125816765767
2225	28-29	0.35876797417024164
2225	30-31	0.3424596885876383
2225	32-33	0.275601850727746
2225	34-35	0.2885543307270453
2225	36-37	0.36879943931135273
2225	38-39	0.2792176829020576
2225	40-41	0.2732066812238543
2225	42-43	0.5859850970884111
2225	44-45	0.4303864392568144
2225	46-47	0.4597003828925992
2225	48-49	0.3201767428777842
2225	50-51	0.3669329338276981
2225	52-53	0.27834378430083007
2225	54-55	0.367387370041115
2225	56-57	0.531109264329352
2225	58-59	0.5321080177985849
2225	60-61	0.4084709224741019
2225	62-63	0.43550820751725183
2225	64-65	0.436602827039934
2225	66-67	0.3780047311944017
2225	68-69	0.5823696660380122
2225	70-71	0.6790289730174877
2225	72-73	0.6280479611167635
2225	74-75	0.5084149800904427
2225	76-77	0.553716866462473
2225	78-79	0.5369018928134679
2225	80-81	0.40060501529755044
2225	82-83	0.42883940863405456
2225	84-85	0.41538888386865835
2225	86-87	0.4580269766523841
2225	88-89	0.3392692584211403
2225	90-91	0.3995356007630235
2225	92-93	0.566480654105824
2225	94-95	0.6784696421423533
2225	96-97	0.47451477448380786
2225	98-99	0.7308198885802355
2225	100	0.8589816643750652
2226	1	1.3780219378268104
2226	2	1.6052643259648782
2226	3	0.7691194264216268
2226	4	1.0206944678519214
2226	5	3.164238972792898
2226	6	-0.2431562415838009
2226	7	0.1959715136495106
2226	8	0.20215100183106216
2226	9	0.221602688085774
2226	10-11	0.06130813520235279
2226	12-13	0.1905337044397939
2226	14-15	0.25754675618224354
2226	16-17	0.32213142716056353
2226	18-19	0.2491757186432153
2226	20-21	0.12695710233870727
2226	22-23	0.2810139820174271
2226	24-25	0.19820765538067775
2226	26-27	0.21402650853287497
2226	28-29	0.17609342670071726
2226	30-31	0.24604299113658357
2226	32-33	0.23607469889915222
2226	34-35	0.24681070309498665
2226	36-37	0.2474473890712332
2226	38-39	0.11944671873508383
2226	40-41	0.17143350058108098
2226	42-43	0.29230205312831004
2226	44-45	0.28834728151762334
2226	46-47	0.14052786719256005
2226	48-49	0.20602823899895384
2226	50-51	0.2199067055306898
2226	52-53	0.2979226538981692
2226	54-55	0.41356394928012463
2226	56-57	0.23576386351664524
2226	58-59	0.18338249138558638
2226	60-61	0.267724708953601
2226	62-63	0.26428545169900275
2226	64-65	0.3418484864414921
2226	66-67	0.2195267112461181
2226	68-69	0.4043127764923966
2226	70-71	0.5436392426887124
2226	72-73	0.33898257508055707
2226	74-75	0.42363478060762105
2226	76-77	0.5322909336955703
2226	78-79	0.33668024523311857
2226	80-81	0.32598366324731387
2226	82-83	0.29400379725613845
2226	84-85	0.3564675687596832
2226	86-87	0.3593938033978574
2226	88-89	0.2720361590491436
2226	90-91	0.27670589998726314
2226	92-93	0.27630703016788516
2226	94-95	0.3572653569558
2226	96-97	0.46823475970730044
2226	98-99	0.43159565511145814
2226	100	0.5542162413975973
2227	1	1.299846274753369
2227	2	1.3997705569192114
2227	3	0.6979817828294905
2227	4	-0.07941163056972123
2227	5	3.1196856966254067
2227	6	-0.0033906254221527377
2227	7	0.28131820143023134
2227	8	0.42937896444950496
2227	9	0.34303367977662447
2227	10-11	0.3632591147851514
2227	12-13	0.22383240360143475
2227	14-15	0.3111206913829889
2227	16-17	0.2094003948593368
2227	18-19	0.01643790682796009
2227	20-21	0.17598780416989968
2227	22-23	0.24294085388183362
2227	24-25	0.24424233458235278
2227	26-27	0.1541970192868405
2227	28-29	0.17721528478233495
2227	30-31	0.17100942017280119
2227	32-33	0.1356972392405993
2227	34-35	0.06872705962459946
2227	36-37	0.16808121109107077
2227	38-39	0.15696968889049145
2227	40-41	0.16617875179417751
2227	42-43	0.31387975361388243
2227	44-45	0.1613086671958115
2227	46-47	0.2756073214847703
2227	48-49	0.09196652902150504
2227	50-51	0.19335833429573768
2227	52-53	0.20073641628384564
2227	54-55	0.34191061510178145
2227	56-57	0.4220599195294952
2227	58-59	0.2906052727764177
2227	60-61	0.21617399588159003
2227	62-63	0.23239873434858538
2227	64-65	0.18450177531188672
2227	66-67	0.1997035122994859
2227	68-69	0.14662606119890853
2227	70-71	0.41623559364794716
2227	72-73	0.42073944208338787
2227	74-75	0.2818293896292374
2227	76-77	0.3159952501529446
2227	78-79	0.33124857308919076
2227	80-81	0.1689608483341587
2227	82-83	0.14679029148577172
2227	84-85	0.14810418069268394
2227	86-87	0.27569182182846674
2227	88-89	0.2836476403604564
2227	90-91	0.3365366400661358
2227	92-93	0.06738688250289471
2227	94-95	0.43742796800543005
2227	96-97	0.32071375752889963
2227	98-99	0.4096595910154406
2227	100	0.3085024981079698
2228	1	1.258454787337545
2228	2	1.4748473946805696
2228	3	-0.0752285877247374
2228	4	-0.18170591835718497
2228	5	3.123025861224246
2228	6	0.07132396519944706
2228	7	0.2653287356665004
2228	8	0.3117090185517881
2228	9	0.33573870439042963
2228	10-11	0.21922339095610965
2228	12-13	0.19344193696226597
2228	14-15	0.2279806403777016
2228	16-17	0.19376479088489873
2228	18-19	0.16106312079415375
2228	20-21	0.18945789138872726
2228	22-23	0.23175256027828794
2228	24-25	0.16569941220946305
2228	26-27	0.23472349631042277
2228	28-29	0.21156685122170416
2228	30-31	0.17841564120840303
2228	32-33	0.25115680675776275
2228	34-35	0.19868098491895125
2228	36-37	0.19513570580661366
2228	38-39	0.17387511443585169
2228	40-41	0.18647423477359837
2228	42-43	0.4735594667678171
2228	44-45	0.4042346457941619
2228	46-47	0.1971932539972272
2228	48-49	0.29294381366614175
2228	50-51	0.13618939072690495
2228	52-53	0.30825701349968426
2228	54-55	0.24069104973126798
2228	56-57	0.33396203006629577
2228	58-59	0.31836651277968286
2228	60-61	0.3222374986996286
2228	62-63	0.281196303044176
2228	64-65	0.3693910663249085
2228	66-67	0.31515888714142903
2228	68-69	0.1887764228311184
2228	70-71	0.4966664776763139
2228	72-73	0.42955817540353536
2228	74-75	0.4057717174248836
2228	76-77	0.4865377655568395
2228	78-79	0.6008222171500748
2228	80-81	0.5345949708966771
2228	82-83	0.3540160597795321
2228	84-85	0.33786787592656253
2228	86-87	0.3851147755513935
2228	88-89	0.39644066643218423
2228	90-91	0.24747303774194052
2228	92-93	0.3502930940351021
2228	94-95	0.421682096672491
2228	96-97	0.44803384424460546
2228	98-99	0.5255468114059312
2228	100	0.5404217259401776
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	27.0
14	166.0
15	552.0
16	1906.0
17	4849.0
18	8005.0
19	10382.0
20	11949.0
21	13889.0
22	16053.0
23	18173.0
24	20547.0
25	23608.0
26	26611.0
27	30014.0
28	33823.0
29	38531.0
30	44240.0
31	51061.0
32	59795.0
33	71665.0
34	88391.0
35	113345.0
36	158806.0
37	262317.0
38	646453.0
39	1244051.0
40	1618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.206434618663373	18.523208559044143	20.690032339980245	34.58032448231224
2	18.597720187223917	51.834797721187	12.908219289928798	16.659262801660287
3	41.45382711309988	15.679113798347935	23.224804155619804	19.642254932932378
4	18.231322304465532	15.978695383844322	22.753138108777108	43.03684420291304
5	17.741794663648857	34.024836946307246	26.695118586321488	21.538249803722408
6	17.229373524585682	28.671257362908936	29.942662950388755	24.156706162116627
7	20.2748086210183	26.372243616146346	35.96488597238384	17.388061790451513
8	21.263694444111206	28.56638714605052	32.87523010131789	17.29468830852038
9	20.921924840012103	28.58694797344221	27.163185655273903	23.327941531271783
10-11	19.542150029391653	30.521965095246284	28.74823550111768	21.18764937424438
12-13	24.045287804930066	26.824998367130405	27.388321716632475	21.741392111307057
14-15	21.827517651653597	27.48071214441765	28.702665109775488	21.98910509415326
16-17	20.70576956144455	29.74858473526852	28.472080595777467	21.073565107509467
18-19	21.29052015894153	29.524265108709123	29.773877552108534	19.41133718024081
20-21	22.609145669296588	28.967023365364238	29.80451884997994	18.61931211535924
22-23	21.68200897611745	30.439488518594054	28.628902724959442	19.249599780329053
24-25	22.192680612039513	28.46811505532429	26.73713998557743	22.602064347058768
26-27	20.06593460790469	26.84837506148254	29.973204276652098	23.112486053960676
28-29	19.55995401962547	28.34181233255761	30.579231753184228	21.519001894632698
30-31	20.118452150140484	30.01859834066341	28.386156942266826	21.47679256692928
32-33	20.623509589943122	30.030371374529636	28.861886557550985	20.484232477976256
34-35	21.339206332068358	29.190309951973642	30.0922704618555	19.3782132541025
36-37	21.772866505912322	28.277635818094325	28.761531724990103	21.18796595100325
38-39	21.385595166130926	29.239032852954487	29.179282637513804	20.196089343400782
40-41	20.924353141749634	29.78338862947762	29.87689665330584	19.415361575466907
42-43	20.425316231287173	29.87727308550462	29.40778936097928	20.289621322228925
44-45	20.420067367534287	30.36534290928043	28.842192150762518	20.372397572422766
46-47	19.873119740121076	28.345811191882646	28.876256255869304	22.904812812126973
48-49	20.875671309348146	28.190044027496192	30.20817087947023	20.72611378368543
50-51	21.778398268225157	30.69216675797407	28.14860579595395	19.380829177846827
52-53	20.450848346087945	30.24497223165949	28.27986914864139	21.02431027361117
54-55	22.173113400769868	28.640118016713924	28.15910858184293	21.027660000673276
56-57	21.348948958156626	28.835170416654034	29.248168387991463	20.56771223719788
58-59	21.12310938814675	28.185904173281788	29.084122384980994	21.606864053590467
60-61	19.60339504372935	30.498326304757494	29.48461183433333	20.413666817179827
62-63	20.431717136705704	31.229244849123134	27.48073628710264	20.858301727068515
64-65	19.804605361814154	30.360547844777315	28.698568738857322	21.136278054551212
66-67	20.115487201634735	30.34491537736616	29.482710243624144	20.05688717737496
68-69	20.863074886732168	30.01494242301853	29.202873050720264	19.91910963952904
70-71	19.522622241949954	31.11366371950366	29.230648495827182	20.1330655427192
72-73	19.983558002860523	30.775459769425485	28.64839718853179	20.592585039182207
74-75	19.98590733565001	29.388053792722868	30.24517703638833	20.380861835238793
76-77	19.480867433648843	30.845889767306083	28.64679764280278	21.026445156242293
78-79	19.57755664058074	29.452352198642252	28.593012917664716	22.37707824311229
80-81	20.50513463964382	29.683970523621106	28.61550427404828	21.195390562686793
82-83	20.37291409239776	28.46391624778563	29.67705504969922	21.486114610117394
84-85	20.659132806283015	29.107750065648023	28.47817886386933	21.754938264199634
86-87	21.334124441991808	28.43312499250213	30.015375633320136	20.217374932185926
88-89	20.693639672999534	27.634451815684347	30.001279636583213	21.67062887473291
90-91	20.101357881028452	27.99238349642035	30.273602296947665	21.632656325603527
92-93	20.126750669476536	28.538761658355693	30.220733978216852	21.11375369395092
94-95	20.143229322872305	28.990250102304277	30.222633438770057	20.64388713605336
96-97	20.10873911809046	28.81403348115802	30.03733606322471	21.039891337526807
98-99	21.1500433876779	29.20977109167663	29.412280245743545	20.22790527490193
100	20.56439304570835	30.050665610966487	28.521133331467198	20.863808011857966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	8.0
2	20.0
3	32.0
4	38.0
5	37.0
6	46.5
7	52.5
8	55.5
9	62.5
10	79.0
11	98.5
12	118.0
13	133.0
14	149.0
15	203.5
16	279.5
17	352.0
18	491.0
19	798.0
20	1312.0
21	2093.5
22	3272.0
23	5052.0
24	7622.5
25	11147.5
26	16033.0
27	21826.5
28	28235.5
29	35539.5
30	44495.0
31	54984.5
32	66175.0
33	78881.5
34	92760.5
35	107172.0
36	121377.5
37	132081.5
38	139093.0
39	144375.0
40	147735.0
41	148001.5
42	145750.5
43	141994.0
44	138754.5
45	134500.5
46	125737.5
47	114799.5
48	105964.5
49	101143.5
50	96529.0
51	89068.0
52	78795.0
53	66207.5
54	53973.0
55	43453.0
56	33931.5
57	25431.5
58	18283.5
59	13326.0
60	10623.5
61	9098.0
62	7441.5
63	5535.0
64	4099.5
65	3417.5
66	3387.0
67	3123.0
68	2409.5
69	2332.0
70	2792.0
71	2574.0
72	1567.5
73	733.5
74	380.5
75	229.0
76	163.5
77	130.0
78	101.5
79	78.5
80	62.5
81	47.5
82	32.0
83	26.0
84	27.5
85	28.5
86	19.5
87	14.5
88	15.0
89	15.0
90	13.0
91	14.5
92	21.5
93	17.5
94	9.5
95	11.0
96	12.0
97	18.5
98	39.5
99	93.0
100	101.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.342378764430903
2	0.005565086182190924
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.166051508038384E-4
30-31	0.010513680781324771
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.664206032153536E-4
40-41	0.0013329547741774668
42-43	8.330967338609168E-5
44-45	0.0
46-47	0.002332670854810567
48-49	0.0
50-51	0.0
52-53	0.0014162644475635587
54-55	0.019960997743307566
56-57	0.011763325882116146
58-59	0.015578908923199146
60-61	2.332670854810567E-4
62-63	0.013062956786939176
64-65	6.664773870887336E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.832528895127117E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3000852.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.86374000686644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.37827749560206	64.66600908671715
2	8.479002344758598	12.69539654111237
3	2.357345548995004	5.294391128589174
4	1.0069803813390228	3.0154526984231924
5	0.5219701035272717	1.953831706091141
6	0.3166754368689737	1.4224504543391814
7	0.20193608690007922	1.058238349538406
8	0.14248500844780188	0.8533568501849924
9	0.10262065597476419	0.6914309497405947
>10	0.4610678175280223	5.8079149258671565
>50	0.022287154521616736	1.1299937747985627
>100	0.008899272446681731	1.180367813435246
>500	4.5269309006666343E-4	0.23116572116295198
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	6.664773870887334E-5	0.0	0.0	0.0	0.0
12-13	6.664773870887334E-5	0.0	0.0	0.0	0.0
14-15	6.664773870887334E-5	0.0	0.0	0.0	0.0
16-17	6.664773870887334E-5	0.0	0.0	0.0	0.0
18-19	6.664773870887334E-5	0.0	0.0	0.0	0.0
20-21	6.664773870887334E-5	0.0	0.0	0.0	0.0
22-23	6.664773870887334E-5	0.0	0.0	0.0	0.0
24-25	6.664773870887334E-5	0.0	0.0	0.0	0.0
26-27	6.664773870887334E-5	0.0	0.0	0.0	0.0
28-29	6.664773870887334E-5	0.0	0.0	0.0	0.0
30-31	6.664773870887334E-5	0.0	0.0	0.0	0.0
32-33	6.664773870887334E-5	0.0	0.0	0.0	0.0
34-35	6.664773870887334E-5	0.0	0.0	0.0	0.0
36-37	1.3329547741774668E-4	0.0	0.0	0.0	0.0
38-39	1.3329547741774668E-4	0.0	0.0	0.0	0.0
40-41	1.3329547741774668E-4	0.0	0.0	0.0	0.0
42-43	1.3329547741774668E-4	0.0	0.0	0.0	0.0
44-45	1.3329547741774668E-4	0.0	0.0	0.0	0.0
46-47	1.4995741209496502E-4	0.0	0.0	0.0	0.0
48-49	1.832812814494017E-4	0.0	0.0	0.0	0.0
50-51	2.3326708548105672E-4	0.0	0.0	3.332386935443667E-5	0.0
52-53	2.4992902015827503E-4	0.0	0.0	3.332386935443667E-5	0.0
54-55	2.832528895127117E-4	3.332386935443667E-5	0.0	3.332386935443667E-5	0.0
56-57	3.165767588671484E-4	3.332386935443667E-5	0.0	3.332386935443667E-5	0.0
58-59	3.8322449757602177E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
60-61	4.831961056393318E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
62-63	5.998296483798601E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
64-65	6.998012564431701E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
66-67	7.831109298292618E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
68-69	8.164347991836986E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
70-71	8.664206032153536E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
72-73	8.997444725697902E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
74-75	9.330683419242269E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
76-77	9.830541459558818E-4	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
78-79	0.0011163496233736285	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
80-81	0.0015662218596585236	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
82-83	0.0017994889451395804	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
84-85	0.0019661082919117637	3.332386935443667E-5	0.0	6.664773870887334E-5	0.0
86-87	0.0019994321612662003	1.3329547741774668E-4	0.0	6.664773870887334E-5	0.0
88	0.0019994321612662003	1.3329547741774668E-4	0.0	6.664773870887334E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	11115	0.0	81.03784	8
CACCGGC	10975	0.0	78.22247	9
CTTCACC	12535	0.0	74.02948	6
CGGGCCC	930	0.0	71.163704	7
CCTTCAC	14210	0.0	69.333115	5
AGGCTGC	55	1.6916601E-10	68.27652	2
TTCACCG	13650	0.0	68.051155	7
GGGCCCG	980	0.0	67.5329	8
GGCCCGA	995	0.0	66.04308	9
GTGTGGC	4530	0.0	64.7596	7
AACGGGC	1010	0.0	64.59751	5
CGTAGTG	2385	0.0	64.15811	4
ACGGGCC	1030	0.0	63.798897	6
CGGGGCA	1145	0.0	62.720325	94
CAGCTGC	95	0.0	61.317825	1
ACCTTCA	5555	0.0	58.809505	4
GCCTTCA	4345	0.0	58.334614	4
TGTGGCA	5190	0.0	57.247787	8
GCGTAGT	1660	0.0	56.83428	3
ATCGGAA	4680	0.0	55.061604	94
>>END_MODULE