FastQCFastQC Report
Tue 7 Mar 2017
1SN_5A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_5A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3000852
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG111150.081.037848
CACCGGC109750.078.222479
CTTCACC125350.074.029486
CGGGCCC9300.071.1637047
CCTTCAC142100.069.3331155
AGGCTGC551.6916601E-1068.276522
TTCACCG136500.068.0511557
GGGCCCG9800.067.53298
GGCCCGA9950.066.043089
GTGTGGC45300.064.75967
AACGGGC10100.064.597515
CGTAGTG23850.064.158114
ACGGGCC10300.063.7988976
CGGGGCA11450.062.72032594
CAGCTGC950.061.3178251
ACCTTCA55550.058.8095054
GCCTTCA43450.058.3346144
TGTGGCA51900.057.2477878
GCGTAGT16600.056.834283
ATCGGAA46800.055.06160494
Produced by FastQC (version 0.11.5)