FastQCFastQC Report
Tue 7 Mar 2017
1SN_6A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_6A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3354310
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA167390.49902960668513047No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG99760.2974084088828999No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT82750.24669753242842787No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA71400.21286046906815412No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA67380.20087588803658576No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC65960.19664252856772332No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG65070.19398922580202785No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG61320.1828095793173559No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG59720.1780395968172292No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG58430.174193798426502No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA54450.16232846695743683No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG52300.1559188029728916No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA50980.15198356741028707No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT49440.14739245925391511No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA47120.1404759846287314No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA45930.13692831014426216No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA43030.1282827168627825No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42720.12735853275338296No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC42550.1268517221127445No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42540.1268219097221187No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA39920.11901106337816124No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA38980.11620869865933679No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG35680.10637060975282547No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA35650.1062811725809481No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34160.10183912637770509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAA1050.087.9997943
GCAGCTT401.8189894E-1187.999793
GCAGCCG401.8189894E-1187.999793
GCAGCAT700.087.999793
AGCAGCC850.087.999792
CAGCTGC7150.086.769021
CAGCAGC4450.086.022271
CTGCAGC1800.085.555361
AGCTGCC3150.085.206152
AGCTGCA1550.085.161092
GCTGCCC2250.084.088693
AGCTGCT1850.083.243042
CAGCCCT182000.082.487721
GCTGCTA700.081.714093
CAGCGTA73000.081.188851
GCTGCAG600.080.666483
TGCAGCT600.080.666482
AGCAGCA2300.080.347632
AGCCCTT154200.080.124332
GCCCTTC142950.077.811643
Produced by FastQC (version 0.11.5)