FastQCFastQC Report
Tue 7 Mar 2017
1SN_6A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_6A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3354310
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG126000.077.224728
CACCGGC126000.074.542539
GTGTGGC54200.071.879727
CTTCACC142600.071.0987246
CCTTCAC158000.068.565615
CGTAGTG30850.068.315424
GGCGCGC6650.066.3489156
CGGGGCA14850.064.7970294
GCGTAGT20550.064.640123
TTCACCG156750.064.351187
TGTGGCA62150.062.6851358
AGGCGCG7100.062.14375
TGTGTGG59250.060.2870336
CCGCGTA3000.060.0063631
CAGCGTA13000.059.463321
CGGGCCC9300.059.051447
CAGCTGC1350.058.8297651
ACCTTCA60900.058.576564
GCCTTCA46300.057.9886054
GGGCCCG9500.056.820088
Produced by FastQC (version 0.11.5)