##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_6A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3354310
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.8024153998885	12.0	12.0	12.0	2.0	32.0
2	14.570124705229988	12.0	12.0	12.0	12.0	22.0
3	13.714926467738522	12.0	12.0	12.0	12.0	22.0
4	13.77053402935328	12.0	12.0	12.0	12.0	22.0
5	22.427828674153552	22.0	22.0	22.0	22.0	27.0
6	26.82289353100936	27.0	22.0	32.0	22.0	32.0
7	34.928745405165294	37.0	37.0	37.0	27.0	41.0
8	37.55825311315889	41.0	37.0	41.0	27.0	41.0
9	38.50742805524832	41.0	37.0	41.0	32.0	41.0
10-11	38.87548005402005	41.0	41.0	41.0	37.0	41.0
12-13	39.055804770578746	41.0	41.0	41.0	37.0	41.0
14-15	39.00638998780673	41.0	41.0	41.0	37.0	41.0
16-17	38.91067745676458	41.0	41.0	41.0	37.0	41.0
18-19	38.845972644150365	41.0	41.0	41.0	37.0	41.0
20-21	38.85633036302548	41.0	41.0	41.0	37.0	41.0
22-23	38.844973929064395	41.0	41.0	41.0	34.5	41.0
24-25	38.8621339411086	41.0	41.0	41.0	37.0	41.0
26-27	38.831620064931386	41.0	41.0	41.0	37.0	41.0
28-29	38.77797713985887	41.0	41.0	41.0	34.5	41.0
30-31	38.646319064129436	41.0	41.0	41.0	32.0	41.0
32-33	38.703517713031886	41.0	41.0	41.0	32.0	41.0
34-35	38.67390774853845	41.0	41.0	41.0	32.0	41.0
36-37	38.598222436208935	41.0	41.0	41.0	32.0	41.0
38-39	38.50200220015443	41.0	41.0	41.0	32.0	41.0
40-41	38.55135586752566	41.0	41.0	41.0	32.0	41.0
42-43	38.297393502687584	41.0	41.0	41.0	32.0	41.0
44-45	38.40272917529984	41.0	41.0	41.0	32.0	41.0
46-47	38.417241847056474	41.0	41.0	41.0	32.0	41.0
48-49	38.3702588609878	41.0	41.0	41.0	32.0	41.0
50-51	38.24445489534361	41.0	41.0	41.0	32.0	41.0
52-53	38.221871115072844	41.0	41.0	41.0	32.0	41.0
54-55	38.08704964657411	41.0	37.0	41.0	32.0	41.0
56-57	37.873162140648894	41.0	37.0	41.0	27.0	41.0
58-59	37.8729859494203	41.0	37.0	41.0	29.5	41.0
60-61	37.87541387051286	41.0	37.0	41.0	29.5	41.0
62-63	37.87560869448561	41.0	37.0	41.0	29.5	41.0
64-65	37.840361206924825	41.0	37.0	41.0	27.0	41.0
66-67	37.73150990814803	41.0	37.0	41.0	27.0	41.0
68-69	37.73050508152198	41.0	37.0	41.0	27.0	41.0
70-71	37.71083635680662	41.0	37.0	41.0	27.0	41.0
72-73	37.57864091273615	41.0	37.0	41.0	27.0	41.0
74-75	37.47536005914778	41.0	37.0	41.0	27.0	41.0
76-77	36.44165551186384	39.0	34.5	41.0	24.5	41.0
78-79	36.96069638763263	41.0	37.0	41.0	27.0	41.0
80-81	37.619656650697166	41.0	37.0	41.0	27.0	41.0
82-83	37.80559742540194	41.0	37.0	41.0	27.0	41.0
84-85	37.71294260220433	41.0	37.0	41.0	27.0	41.0
86-87	37.58263085403556	41.0	37.0	41.0	27.0	41.0
88-89	37.48479016548858	41.0	37.0	41.0	27.0	41.0
90-91	37.33439992129529	41.0	37.0	41.0	27.0	41.0
92-93	37.23666655735457	41.0	37.0	41.0	27.0	41.0
94-95	37.07679388607493	41.0	37.0	41.0	27.0	41.0
96-97	37.01419815103553	41.0	37.0	41.0	27.0	41.0
98-99	36.89689339983484	41.0	37.0	41.0	27.0	41.0
100	36.77138338436221	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5582053460518246
1101	2	-0.47566124259475906
1101	3	-0.008329565852225329
1101	4	0.05216099290548826
1101	5	-1.0552546592280336
1101	6	-0.879780899391303
1101	7	-0.24737755071968337
1101	8	-0.16877344325506272
1101	9	-0.048011430295900936
1101	10-11	-0.058985658087010506
1101	12-13	0.01659633606315225
1101	14-15	0.049108924953543465
1101	16-17	-0.014216105428175752
1101	18-19	0.17998496757287796
1101	20-21	0.088548289603402
1101	22-23	-0.016592101708141627
1101	24-25	0.0681660752942932
1101	26-27	0.10326633272274677
1101	28-29	-0.04470262760940358
1101	30-31	-0.048427672295964896
1101	32-33	-0.10576691131225147
1101	34-35	-0.0052604755912071255
1101	36-37	0.028235495524981502
1101	38-39	0.015407312998718226
1101	40-41	-0.051042238266951756
1101	42-43	0.06177386776856508
1101	44-45	-0.010366487276229464
1101	46-47	0.00636381757556137
1101	48-49	0.08044465537387424
1101	50-51	0.05489600227636515
1101	52-53	0.06445170364592201
1101	54-55	0.022801328993615755
1101	56-57	0.08910913015866839
1101	58-59	0.06152139035055626
1101	60-61	0.07041884742397286
1101	62-63	-0.19203343785819982
1101	64-65	0.021796433472161425
1101	66-67	-0.12259963627424497
1101	68-69	0.05454878013908626
1101	70-71	-0.024265067655200312
1101	72-73	-0.009036100224236066
1101	74-75	0.09099656838214543
1101	76-77	-0.3378674036508542
1101	78-79	-0.28656937193690624
1101	80-81	-0.13004220118130405
1101	82-83	-0.10547678515698067
1101	84-85	0.050871866289867285
1101	86-87	-0.10716611658246933
1101	88-89	-0.35346007967206816
1101	90-91	-0.19954994510560198
1101	92-93	-0.13600443315846178
1101	94-95	-0.045738105507084015
1101	96-97	-0.2301424070367446
1101	98-99	-0.3901193957439091
1101	100	-0.537211170224154
1102	1	1.7228797022182647
1102	2	0.933502703408168
1102	3	-0.09962446533265457
1102	4	-0.02565681302845846
1102	5	-0.8005237746626399
1102	6	-0.10224010649266191
1102	7	0.09186823694232515
1102	8	-0.1549186977010848
1102	9	-0.018491802284835046
1102	10-11	-0.08330122778887983
1102	12-13	-0.1032375584603713
1102	14-15	-0.015068377035021285
1102	16-17	-0.05865145256439064
1102	18-19	-0.025771069912579492
1102	20-21	0.024813287368871784
1102	22-23	0.027651007933755523
1102	24-25	-0.09192084948574575
1102	26-27	-0.008790557294211965
1102	28-29	-0.10210556217359823
1102	30-31	-0.22085890517454487
1102	32-33	-0.13712840308885177
1102	34-35	-0.06329641223300797
1102	36-37	-0.05575540261814638
1102	38-39	-0.07435002767613241
1102	40-41	-0.08361770896735976
1102	42-43	-0.09786785749044924
1102	44-45	-0.013485101392298304
1102	46-47	-0.31663815767748815
1102	48-49	-0.2704085584208258
1102	50-51	-0.16243402873408286
1102	52-53	-0.15902702741603747
1102	54-55	-0.31903326829697676
1102	56-57	-0.03638033345266223
1102	58-59	0.04057057381027818
1102	60-61	0.037168242737266155
1102	62-63	-0.20524852892794598
1102	64-65	-0.22130399739909734
1102	66-67	-0.20441400342568272
1102	68-69	-0.36882317126764264
1102	70-71	-0.4014976323797086
1102	72-73	-0.08562986333715372
1102	74-75	-0.40624609435449344
1102	76-77	-0.09913402187435594
1102	78-79	-0.11625872160445994
1102	80-81	-0.2460340750107335
1102	82-83	-0.26291878128834867
1102	84-85	-0.27428548871325376
1102	86-87	-0.0585533992278755
1102	88-89	-0.24061377073430634
1102	90-91	-0.3402944005805324
1102	92-93	-0.22938409453391273
1102	94-95	-0.045180326796831594
1102	96-97	-0.05979593605318456
1102	98-99	-0.24818552407735694
1102	100	-0.651170307389954
1103	1	1.4039731824482917
1103	2	0.7241446578452315
1103	3	0.5596933481607369
1103	4	0.9766003904503151
1103	5	2.86321011123524
1103	6	-0.20180455060932445
1103	7	-0.46550264695293464
1103	8	-0.27221054519932153
1103	9	-0.1548875005263426
1103	10-11	-0.1588008902366198
1103	12-13	-0.33745840841130104
1103	14-15	-0.4007160471679754
1103	16-17	-0.1880736708784525
1103	18-19	-0.2399517634359256
1103	20-21	-0.24275621341308096
1103	22-23	-0.10772896056137427
1103	24-25	-0.24295114031018983
1103	26-27	-0.3844043209168504
1103	28-29	-0.12086524198117132
1103	30-31	-0.2729608535224344
1103	32-33	-0.264922309939557
1103	34-35	-0.32475593338156017
1103	36-37	-0.17922648942297315
1103	38-39	-0.04546815300352591
1103	40-41	-0.24286165335539067
1103	42-43	-0.3070873837070067
1103	44-45	-0.41030828073750314
1103	46-47	-0.37869092456597997
1103	48-49	-0.3881052086164587
1103	50-51	-0.3521261092095713
1103	52-53	-0.08570384428873723
1103	54-55	-0.22601173586642886
1103	56-57	-0.23763263344113028
1103	58-59	-0.3068405563373915
1103	60-61	-0.12269289279989692
1103	62-63	-0.1436420496264219
1103	64-65	-0.28495529172765544
1103	66-67	-0.43319655074445507
1103	68-69	-0.33032708479375117
1103	70-71	-0.6150439971214823
1103	72-73	-0.37454044413505727
1103	74-75	-0.2860397856255972
1103	76-77	-0.4781755483670054
1103	78-79	-0.513061453981301
1103	80-81	-0.26916586033127743
1103	82-83	-0.36984295211059504
1103	84-85	-0.3611320366976756
1103	86-87	-0.22058144064941132
1103	88-89	-0.2375727274046895
1103	90-91	-0.5769519854839018
1103	92-93	-0.3670763433127604
1103	94-95	-0.3673563522654817
1103	96-97	-0.3628516822895236
1103	98-99	-0.31964673298722346
1103	100	-0.22074956787629674
1104	1	1.6211788757931558
1104	2	-0.42554215085245595
1104	3	0.05978114206127039
1104	4	-0.03120609984815914
1104	5	-1.1338437165788235
1104	6	-0.5158853783326087
1104	7	-0.16421560031586324
1104	8	-0.12019952736342532
1104	9	-0.051701119450648036
1104	10-11	-0.1347331778470533
1104	12-13	-0.07759503244317756
1104	14-15	-0.0668531035997475
1104	16-17	0.09367009515737834
1104	18-19	-0.00663164895745183
1104	20-21	-0.018470509365720034
1104	22-23	0.09220689613987076
1104	24-25	-0.02740612533147413
1104	26-27	6.241911975095604E-4
1104	28-29	0.05665521937279294
1104	30-31	-0.014564531549353887
1104	32-33	-0.026221384938239112
1104	34-35	-0.08408944031189947
1104	36-37	-0.20826978674687524
1104	38-39	-0.14221470216572385
1104	40-41	-0.1278660370780429
1104	42-43	-0.04955406980379706
1104	44-45	-0.05771328415369936
1104	46-47	-0.26379026903818215
1104	48-49	-0.3119725478520863
1104	50-51	-0.08658527489278356
1104	52-53	-0.1238913840050273
1104	54-55	-0.1705102833850276
1104	56-57	-0.0014695494026568667
1104	58-59	-0.029211576944469186
1104	60-61	-0.038640744417456574
1104	62-63	-0.12490247141937516
1104	64-65	-0.12873064445607696
1104	66-67	-0.49360072519511533
1104	68-69	-0.44329406473134725
1104	70-71	-0.17799598639987124
1104	72-73	-0.12185807171692176
1104	74-75	-0.21558637492214672
1104	76-77	-0.2910969590895647
1104	78-79	-0.032679568431099426
1104	80-81	-0.2853691503927678
1104	82-83	-0.1839408608240518
1104	84-85	-0.1755076010870411
1104	86-87	-0.143636059205825
1104	88-89	-0.1895231853663475
1104	90-91	-0.1994692098110562
1104	92-93	-0.23305113033789837
1104	94-95	-0.19529147555918058
1104	96-97	-0.07753345030339176
1104	98-99	-0.20461468522734094
1104	100	-0.3074856414941678
1105	1	1.6650295174992813
1105	2	0.2804069002555245
1105	3	0.08235149544074893
1105	4	-0.21553499123104558
1105	5	-1.0595029947787502
1105	6	0.43650485766830727
1105	7	0.07388644209430595
1105	8	0.07055134256273021
1105	9	0.23469100826000755
1105	10-11	-0.019915941883738242
1105	12-13	0.028659179792761336
1105	14-15	0.056525999081912914
1105	16-17	0.026423574138860317
1105	18-19	0.07372262720323874
1105	20-21	0.027193454720723764
1105	22-23	0.14152874423037076
1105	24-25	0.09545174478847684
1105	26-27	-0.013038349767839463
1105	28-29	-0.005053567242924828
1105	30-31	-0.04554055543001567
1105	32-33	-0.09089095762588073
1105	34-35	-0.013743870644049139
1105	36-37	-0.10006254329191933
1105	38-39	-0.08499916006361019
1105	40-41	0.07645076356062219
1105	42-43	0.09879907248805608
1105	44-45	0.18030892463436032
1105	46-47	-0.06482376734172135
1105	48-49	-0.21949715598758957
1105	50-51	-0.13868799415322997
1105	52-53	-0.09517442022808353
1105	54-55	0.015308835610454707
1105	56-57	0.06891767258976955
1105	58-59	-0.01204995146250809
1105	60-61	-0.08127074161170356
1105	62-63	-0.1963758356255738
1105	64-65	-0.16931765298033952
1105	66-67	-4.0914474973163806E-4
1105	68-69	-0.2105402964976122
1105	70-71	-0.15877488486943747
1105	72-73	-0.18948669496296588
1105	74-75	-0.19227871754722514
1105	76-77	-0.28779890488947757
1105	78-79	-0.16432521127976685
1105	80-81	-0.23404394119107508
1105	82-83	-0.1161221988471226
1105	84-85	-0.10589962466688263
1105	86-87	-0.19490978150630411
1105	88-89	-0.10252044614750133
1105	90-91	-0.04339621411223504
1105	92-93	-0.02428591928499202
1105	94-95	-0.0691491460950715
1105	96-97	-0.07017082343012504
1105	98-99	-0.16716143562290142
1105	100	-0.1754043847509763
1106	1	1.0798664780915672
1106	2	-0.580933002921741
1106	3	-0.16411184197036732
1106	4	-0.14010585952625298
1106	5	-1.0849274737267258
1106	6	-0.5435723780948258
1106	7	0.0321668739609251
1106	8	-0.10553700534546095
1106	9	-0.11261833651835218
1106	10-11	-0.10251792236939394
1106	12-13	-0.03258834913943787
1106	14-15	0.07153306900262635
1106	16-17	-0.07589247741822192
1106	18-19	0.07981030556837254
1106	20-21	0.013204714392209382
1106	22-23	0.0012791635459024064
1106	24-25	-0.18319283906214423
1106	26-27	-0.0560113251944756
1106	28-29	-0.05901499248447095
1106	30-31	0.15407542190992274
1106	32-33	0.05451898429409141
1106	34-35	-0.10378827610116304
1106	36-37	-0.3408130897071473
1106	38-39	-0.10229402455490799
1106	40-41	-0.09326603107576403
1106	42-43	-0.07004584091392019
1106	44-45	0.030213656601524974
1106	46-47	-0.24986810470971932
1106	48-49	-0.17646579413702312
1106	50-51	-0.2631002147892261
1106	52-53	-0.13332401884269274
1106	54-55	-0.1996128392004337
1106	56-57	-0.15401468767432647
1106	58-59	-0.09703862597152835
1106	60-61	-0.06730617292226526
1106	62-63	-0.19881132685455327
1106	64-65	-0.2831243683836888
1106	66-67	-0.22388105458157526
1106	68-69	-0.23664163808032157
1106	70-71	-0.5337230236532307
1106	72-73	-0.42728926600829453
1106	74-75	-0.31522560349113604
1106	76-77	-0.2299368806906088
1106	78-79	-0.18100239393339024
1106	80-81	-0.19522973598407134
1106	82-83	-0.1922672876920899
1106	84-85	-0.3881045320770298
1106	86-87	-0.3329161092555708
1106	88-89	-0.19560395291896526
1106	90-91	-0.4397510628522525
1106	92-93	-0.34995648367574006
1106	94-95	-0.24059642582108864
1106	96-97	-0.31147476420166953
1106	98-99	-0.49743913711563437
1106	100	-0.5358342767843212
1107	1	1.4683852724726716
1107	2	-0.7011154038724943
1107	3	-0.10973179571977276
1107	4	-0.1715397420340441
1107	5	-1.2291011722270753
1107	6	-0.25963268459743105
1107	7	-0.49071904626114105
1107	8	-0.16447490751089333
1107	9	-0.1858266508544375
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2223	2	-0.8223504407981643
2223	3	0.05172653240372149
2223	4	-0.03952325593622952
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2223	7	0.19027976239456024
2223	8	0.2723693404767715
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2223	32-33	0.36261926736876404
2223	34-35	0.2938072985466036
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2223	38-39	0.2797557678261171
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2223	44-45	0.34167757439561797
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2223	50-51	0.2665061671617508
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2223	64-65	0.48566263435530743
2223	66-67	0.5966488513292987
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2223	76-77	0.6146251476009823
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2223	84-85	0.4046675572981684
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2224	2	-0.9511256300217052
2224	3	-0.13919474496780992
2224	4	-0.26795708686899644
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2224	7	0.4658860539989078
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2224	9	0.36484012178728165
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2224	12-13	0.24978187802599194
2224	14-15	0.19011284397109307
2224	16-17	0.2198186756473035
2224	18-19	0.23797810422705368
2224	20-21	0.17035385223184107
2224	22-23	0.1998463617215691
2224	24-25	0.24041601410242208
2224	26-27	0.3023413635377139
2224	28-29	0.25658210048788277
2224	30-31	0.23855398328498012
2224	32-33	0.23591447151996903
2224	34-35	0.2273701505699961
2224	36-37	0.19214318916370843
2224	38-39	0.1539850222927157
2224	40-41	0.22844060692775514
2224	42-43	0.2819651039976563
2224	44-45	0.37127633509540914
2224	46-47	0.30934302657686885
2224	48-49	0.2185818379632991
2224	50-51	0.27971235821975426
2224	52-53	0.32119521196884193
2224	54-55	0.3291257483005623
2224	56-57	0.49061628454233386
2224	58-59	0.4514247592162448
2224	60-61	0.2692291241535898
2224	62-63	0.22888459898614144
2224	64-65	0.3130082953313078
2224	66-67	0.2788417539612311
2224	68-69	0.2654378665211965
2224	70-71	0.4784776422986212
2224	72-73	0.31478974141723626
2224	74-75	0.32308925366756824
2224	76-77	0.5533629115954213
2224	78-79	0.2716916399986573
2224	80-81	0.33051716095574335
2224	82-83	0.2570389098046988
2224	84-85	0.29633170334152936
2224	86-87	0.39886261046783744
2224	88-89	0.27733977335793014
2224	90-91	0.347284149087713
2224	92-93	0.41206423096416245
2224	94-95	0.3227910938294656
2224	96-97	0.37086502743319016
2224	98-99	0.4298347391016222
2224	100	0.7041744655935958
2225	1	1.42969057191765
2225	2	1.5133235363725408
2225	3	0.8723137694011172
2225	4	1.0361802327145462
2225	5	3.2109247225292386
2225	6	-0.10768193288551942
2225	7	0.2155517201405459
2225	8	0.2734614466092822
2225	9	0.20841397737486744
2225	10-11	0.2202727882950981
2225	12-13	0.11385287567822644
2225	14-15	0.16751187514586974
2225	16-17	0.13502913261977767
2225	18-19	0.12939142012550064
2225	20-21	0.20930374673093155
2225	22-23	0.17473293586444072
2225	24-25	0.22481129083179496
2225	26-27	0.15001390457410224
2225	28-29	0.21052575408828744
2225	30-31	0.1254017846064599
2225	32-33	0.15596781634447865
2225	34-35	0.11478755772424876
2225	36-37	0.1556661269429398
2225	38-39	0.2180582488373375
2225	40-41	0.23665779020214472
2225	42-43	0.3567134868124029
2225	44-45	0.24654256390854812
2225	46-47	0.3214186702314521
2225	48-49	0.18489460604952512
2225	50-51	0.21518592355601385
2225	52-53	0.26403774319809514
2225	54-55	0.2547426561829198
2225	56-57	0.36124765545974924
2225	58-59	0.4325373727659283
2225	60-61	0.3893292757182749
2225	62-63	0.32814336843942726
2225	64-65	0.34088926257154384
2225	66-67	0.5185888489714827
2225	68-69	0.5953746155082911
2225	70-71	0.5847995240781643
2225	72-73	0.40343650655140806
2225	74-75	0.4237447956846623
2225	76-77	0.5264341767147442
2225	78-79	0.4119459318547811
2225	80-81	0.3677941478894766
2225	82-83	0.3483974940122536
2225	84-85	0.31217293686011516
2225	86-87	0.3729665957274335
2225	88-89	0.4087379327881209
2225	90-91	0.4095197776849062
2225	92-93	0.35693010117327617
2225	94-95	0.5991910859045149
2225	96-97	0.4606250004883634
2225	98-99	0.46555273963655
2225	100	0.4881081139437171
2226	1	1.4785700218353863
2226	2	1.34602596625213
2226	3	0.7957155352753027
2226	4	0.9661171751433777
2226	5	3.216352130941722
2226	6	-0.3458442176409271
2226	7	0.14953070532005341
2226	8	0.32064712559249386
2226	9	0.2611696325640054
2226	10-11	0.1420052000177776
2226	12-13	0.16837096376218597
2226	14-15	0.08758738691508938
2226	16-17	0.11497295763173554
2226	18-19	0.10298720929559124
2226	20-21	0.13436110159036474
2226	22-23	0.26583162555170503
2226	24-25	0.29450006697742026
2226	26-27	0.258329375965495
2226	28-29	0.33130427256310213
2226	30-31	0.18155891070194485
2226	32-33	0.18273696795139927
2226	34-35	0.16670632357876514
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2226	38-39	0.2242158665458902
2226	40-41	0.2230787814252082
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2226	46-47	0.19172261821223913
2226	48-49	0.3009226673241798
2226	50-51	0.376609045102434
2226	52-53	0.3621091450819591
2226	54-55	0.29645733706048105
2226	56-57	0.34360382585939675
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2226	60-61	0.35263756955869496
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2226	66-67	0.3605041079860314
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2226	70-71	0.3946345715024435
2226	72-73	0.36668662617726255
2226	74-75	0.4256124337787739
2226	76-77	0.654527406219394
2226	78-79	0.545543097541497
2226	80-81	0.39882935394091135
2226	82-83	0.3467936882715179
2226	84-85	0.20319067426006399
2226	86-87	0.42218378383170574
2226	88-89	0.4439515222846424
2226	90-91	0.4650458364556016
2226	92-93	0.3755650227446026
2226	94-95	0.6012339021264381
2226	96-97	0.5805628605949451
2226	98-99	0.48895008099300696
2226	100	0.6547836296458414
2227	1	1.269650271212388
2227	2	1.673533371294651
2227	3	0.641117535967032
2227	4	-0.16598689896025753
2227	5	3.2760985193206835
2227	6	0.23533422614726263
2227	7	0.4943531616657779
2227	8	0.5305382892040242
2227	9	0.4793199682297029
2227	10-11	0.31945030515164063
2227	12-13	0.2744695927207843
2227	14-15	0.3680103057434039
2227	16-17	0.42218134528349793
2227	18-19	0.33468040330770776
2227	20-21	0.2760469459869199
2227	22-23	0.407299974537608
2227	24-25	0.3636404390918955
2227	26-27	0.40940610018751755
2227	28-29	0.3422974350926964
2227	30-31	0.46937944484051997
2227	32-33	0.23472856965766198
2227	34-35	0.35246968591148686
2227	36-37	0.38404809237733417
2227	38-39	0.35761881614726576
2227	40-41	0.29771917127630587
2227	42-43	0.5151335247880624
2227	44-45	0.39112134403720233
2227	46-47	0.45123344198996307
2227	48-49	0.37465909880314285
2227	50-51	0.4408892483642006
2227	52-53	0.46819569096218316
2227	54-55	0.3995394088760875
2227	56-57	0.5628068858712822
2227	58-59	0.6343484825853452
2227	60-61	0.5500113845301868
2227	62-63	0.6410986889075332
2227	64-65	0.5518527981834183
2227	66-67	0.5656345941470207
2227	68-69	0.5819789904598522
2227	70-71	0.6332257621623327
2227	72-73	0.6005854254074947
2227	74-75	0.5806753544694416
2227	76-77	0.7798854089863099
2227	78-79	0.6461816716052269
2227	80-81	0.4640847392248304
2227	82-83	0.32003199761606993
2227	84-85	0.582519464799347
2227	86-87	0.4256650960761874
2227	88-89	0.5631884481721485
2227	90-91	0.5245899164123244
2227	92-93	0.5019932647786405
2227	94-95	0.7740462552449756
2227	96-97	0.44096871900343615
2227	98-99	0.5351731767441947
2227	100	0.6366975892595406
2228	1	1.48672631580588
2228	2	1.476784602449671
2228	3	-0.0700255499268021
2228	4	0.012766613230443369
2228	5	3.132221438442105
2228	6	0.27424212366682355
2228	7	0.36902082534141556
2228	8	0.3506291529763459
2228	9	0.187596653325933
2228	10-11	0.18790365721665836
2228	12-13	0.25088693161318787
2228	14-15	0.1711793969546207
2228	16-17	0.16621062109243212
2228	18-19	0.13910142055897978
2228	20-21	0.12135005182093295
2228	22-23	0.1877635081791169
2228	24-25	0.2849881384181572
2228	26-27	0.2031706399017139
2228	28-29	0.16152158083728096
2228	30-31	0.23456589307158282
2228	32-33	0.20180083770201662
2228	34-35	0.1836673807058915
2228	36-37	0.16811067734969498
2228	38-39	0.148224272505459
2228	40-41	0.17178258708947425
2228	42-43	0.3343774974156375
2228	44-45	0.25303560126551616
2228	46-47	0.2429492037954546
2228	48-49	0.2009142349099804
2228	50-51	0.15269323035761317
2228	52-53	0.2615769109386221
2228	54-55	0.2482111965433944
2228	56-57	0.42079447613080134
2228	58-59	0.31305573204907233
2228	60-61	0.2480564538676262
2228	62-63	0.2641481012764899
2228	64-65	0.327773143208951
2228	66-67	0.2845941926243185
2228	68-69	0.4852190807949057
2228	70-71	0.4109579066379965
2228	72-73	0.42341428730664177
2228	74-75	0.49003062200018377
2228	76-77	0.4596255446399553
2228	78-79	0.4533293573359103
2228	80-81	0.4756202085743553
2228	82-83	0.24739007489937137
2228	84-85	0.23076318062157952
2228	86-87	0.3457391542048356
2228	88-89	0.39345144705428226
2228	90-91	0.3695776803931139
2228	92-93	0.5121861709087341
2228	94-95	0.7242175217484998
2228	96-97	0.5862487878876905
2228	98-99	0.6294603908594354
2228	100	0.7157936344572775
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	16.0
13	37.0
14	164.0
15	605.0
16	2323.0
17	5640.0
18	9274.0
19	11801.0
20	13946.0
21	16145.0
22	18297.0
23	20551.0
24	23556.0
25	26765.0
26	30527.0
27	34466.0
28	38454.0
29	43955.0
30	50547.0
31	57760.0
32	68220.0
33	81375.0
34	99227.0
35	127755.0
36	179164.0
37	296102.0
38	728084.0
39	1367964.0
40	1590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.42850857823063	19.1388369313792	19.138668753861776	34.29398573652839
2	19.105671786679736	49.992919099142455	13.644076058713935	17.257333055463878
3	40.11221383831548	15.820809644904616	24.488165971541093	19.578810545238813
4	18.16361636223247	15.997626933706188	21.756069057421644	44.0826876466397
5	18.122147326871996	35.69088724655741	25.819169963420197	20.367795463150394
6	17.780884891378555	27.945807036320435	31.006794243823617	23.266513828477393
7	20.63050224934487	26.921244607683846	35.529453151318755	16.91879999165253
8	20.903404873133372	27.786936806675588	33.7391594694587	17.570498850732342
9	21.083203400997522	27.990287123134117	27.814930641473207	23.111578834395154
10-11	19.722893829133266	29.93597789113111	28.906347356088137	21.434780923647487
12-13	24.249801002292575	26.719683034662868	27.30730016009254	21.723215802952023
14-15	21.733024079467906	27.300234623514225	28.68965599482457	22.277085302193296
16-17	21.02348918257406	29.30274780804398	28.413280227528166	21.260482781853792
18-19	21.443992952350857	29.542797177362857	29.700698504312363	19.312511365973926
20-21	22.857830671583724	28.9302270809794	29.705438674421863	18.506503573015014
22-23	21.881340722831222	30.07253354639255	28.777498203803464	19.268627526972757
24-25	22.09163732630556	28.413891381535993	26.74004489746028	22.754426394698164
26-27	20.280862532085585	26.618082407410164	29.947500380107982	23.153554680396265
28-29	20.006111558298304	27.815311689473216	30.550874996273443	21.62770175595504
30-31	20.132597551538343	30.03261643952631	28.08786924562986	21.746916763305485
32-33	21.001174608190656	29.669663805670915	28.69202607987932	20.63713550625911
34-35	21.469676326874975	29.129567630898755	29.732314544571015	19.668441497655255
36-37	22.024350760663147	27.898614021959805	28.8457387659459	21.231296451431145
38-39	21.428389358496	29.357568826587748	28.91862236368653	20.29541945122972
40-41	20.860906520225804	29.639934948297014	29.869300336438343	19.62985819503884
42-43	20.6166460936113	29.68428794446781	29.192740734932837	20.506325226988054
44-45	20.64695868897031	30.12826781066747	28.710181825770427	20.514591674591795
46-47	20.062529896901992	27.9201177138576	28.97827239619878	23.039079993041632
48-49	21.11396978812334	28.33381530031512	30.034194812047783	20.51802009951376
50-51	22.063911803023572	30.51004528502136	28.127647712942455	19.298395199012614
52-53	20.429530825610204	30.23543611423607	28.258654020874026	21.0763790392797
54-55	22.30839589497248	28.35426921221907	28.051856274837363	21.285478617971087
56-57	21.326063645103734	28.679832429243753	29.303444783301007	20.690659142351507
58-59	21.05205093713274	27.933143708794194	29.13650268402856	21.878302670044505
60-61	19.72240403696808	30.340776069638387	29.42633774950627	20.51048214388727
62-63	20.369142679781817	30.97242387185077	27.396309437858562	21.262124010508852
64-65	20.02221024756973	30.107197983488394	28.823028300178205	21.047563468763673
66-67	20.230181468021737	29.984199432968328	29.54373626766757	20.241882831342362
68-69	20.952878535376872	29.826506792753204	29.120832600445397	20.099782071424528
70-71	19.806532491033924	30.54622262104576	29.41153322143749	20.23571166648282
72-73	20.167247511410693	30.642874391454576	28.396838694098044	20.79303940303669
74-75	20.189144712325337	28.938246614057732	30.40103627869815	20.471572394918777
76-77	19.7114905897189	30.242613234912696	28.66000757234722	21.38588860302119
78-79	19.750753508173066	29.30367199215338	28.406766220176426	22.538808279497125
80-81	20.784842165515656	29.417688612096992	28.4416891129459	21.35578010944145
82-83	20.57084765570266	28.005670316697024	29.766136701735974	21.657345325864334
84-85	20.811537991419993	28.753961321404404	28.556975950344484	21.877524736831123
86-87	21.627488216652605	28.504729735772784	29.420655812968988	20.447126234605626
88-89	21.660326564926915	28.580363770790417	29.15271695221968	20.606592712062987
90-91	21.867179837283974	28.972307270347702	28.567961816290087	20.592551076078237
92-93	22.111924658126412	28.912995519197686	28.97744990773065	19.99762991494525
94-95	21.999844975568745	28.886447585345422	28.51824965492158	20.595457784164253
96-97	21.462938726593546	29.385656066374306	28.664449618550464	20.486955588481685
98-99	22.64316655288271	29.715351294304938	28.086655675831988	19.554826476980363
100	22.136415537025496	30.67727789023674	26.96903386985699	20.21727270288077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.0
2	15.0
3	19.5
4	29.0
5	33.0
6	37.0
7	51.0
8	65.0
9	68.5
10	79.0
11	104.0
12	121.5
13	136.0
14	153.0
15	203.5
16	291.5
17	400.5
18	561.5
19	868.0
20	1434.0
21	2280.5
22	3497.5
23	5502.5
24	8494.0
25	12350.0
26	17427.5
27	23808.0
28	30954.5
29	38706.0
30	48252.0
31	59370.5
32	71157.5
33	84112.5
34	98496.0
35	114165.5
36	129299.0
37	140647.0
38	149363.0
39	156693.0
40	160611.0
41	161330.5
42	159815.0
43	156949.5
44	154715.0
45	151204.5
46	141945.0
47	131227.5
48	122883.5
49	117838.0
50	113722.5
51	104886.0
52	92713.5
53	80013.0
54	66780.5
55	53071.5
56	40674.5
57	30045.0
58	21091.5
59	15251.0
60	12238.5
61	10383.5
62	8579.0
63	6468.5
64	4811.5
65	4445.0
66	5609.0
67	5679.0
68	3850.5
69	3238.5
70	4035.5
71	3881.0
72	2294.0
73	916.5
74	430.0
75	264.5
76	162.0
77	111.5
78	77.0
79	63.0
80	53.0
81	44.5
82	32.0
83	29.0
84	28.5
85	28.0
86	32.0
87	23.0
88	18.5
89	17.0
90	15.0
91	20.0
92	20.0
93	19.5
94	17.5
95	17.0
96	18.5
97	19.5
98	41.5
99	97.5
100	111.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.366301862380041
2	0.006469288765796841
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.9812390625791893E-4
30-31	0.009733745539321052
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.496531328350689E-4
40-41	0.0016396814844185541
42-43	1.0434336719027161E-4
44-45	0.0
46-47	0.002221023101621496
48-49	0.0
50-51	0.0
52-53	0.0013564637734735311
54-55	0.018409151211426493
56-57	0.01143305180499119
58-59	0.014593165211325131
60-61	3.1303010157081486E-4
62-63	0.011224365070610648
64-65	7.453097656447973E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4906195312895947E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3354310.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.6670233894983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51506697968661	62.141253723674
2	8.90520056576802	12.942288356016766
3	2.5094530430546707	5.470634489235042
4	1.0794299255411355	3.137558385864883
5	0.5559666665629449	2.020022138145546
6	0.34346626531429575	1.4975202681058533
7	0.22931785986771056	1.166469240064563
8	0.16534440645490103	0.9612068680944804
9	0.11964760744570184	0.7824991939878894
>10	0.5374520792308806	6.648561972982324
>50	0.027008268242077894	1.3380941576793863
>100	0.012061442233708898	1.5633147100502338
>500	5.431126977210306E-4	0.29845693215556135
>1k	4.177789962250203E-5	0.0321195639435635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.981239062579189E-5	0.0
3	0.0	0.0	0.0	5.962478125158378E-5	0.0
4	0.0	0.0	0.0	5.962478125158378E-5	0.0
5	0.0	0.0	0.0	5.962478125158378E-5	0.0
6	0.0	0.0	0.0	5.962478125158378E-5	0.0
7	0.0	0.0	0.0	5.962478125158378E-5	0.0
8	2.981239062579189E-5	0.0	0.0	5.962478125158378E-5	0.0
9	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
10-11	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
12-13	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
14-15	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
16-17	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
18-19	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
20-21	5.962478125158378E-5	0.0	0.0	5.962478125158378E-5	0.0
22-23	5.962478125158378E-5	0.0	1.4906195312895945E-5	5.962478125158378E-5	0.0
24-25	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
26-27	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
28-29	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
30-31	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
32-33	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
34-35	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
36-37	5.962478125158378E-5	0.0	2.981239062579189E-5	5.962478125158378E-5	0.0
38-39	5.962478125158378E-5	0.0	2.981239062579189E-5	7.453097656447973E-5	0.0
40-41	5.962478125158378E-5	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
42-43	5.962478125158378E-5	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
44-45	8.943717187737567E-5	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
46-47	1.1924956250316756E-4	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
48-49	1.1924956250316756E-4	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
50-51	1.3415575781606351E-4	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
52-53	1.4906195312895947E-4	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
54-55	1.639681484418554E-4	0.0	2.981239062579189E-5	8.943717187737567E-5	0.0
56-57	2.235929296934392E-4	0.0	1.7887434375475134E-4	8.943717187737567E-5	0.0
58-59	3.875610781352946E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
60-61	4.471858593868784E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
62-63	4.919044453255662E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
64-65	5.366230312642541E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
66-67	6.707787890803175E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
68-69	7.154973750190055E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
70-71	8.347469375221729E-4	0.0	3.279362968837108E-4	8.943717187737567E-5	0.0
72-73	8.794655234608609E-4	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
74-75	9.241841093995487E-4	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
76-77	0.0010136212812769244	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
78-79	0.0012521204062832594	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
80-81	0.0016545876797314498	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
82-83	0.0018334620234862014	0.0	3.279362968837108E-4	1.1924956250316756E-4	0.0
84-85	0.0019378053906764729	0.0	3.279362968837108E-4	1.4906195312895947E-4	0.0
86-87	0.0020570549531796407	2.981239062579189E-5	3.279362968837108E-4	1.4906195312895947E-4	0.0
88	0.0020570549531796407	2.981239062579189E-5	3.279362968837108E-4	1.4906195312895947E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	12600	0.0	77.22472	8
CACCGGC	12600	0.0	74.54253	9
GTGTGGC	5420	0.0	71.87972	7
CTTCACC	14260	0.0	71.098724	6
CCTTCAC	15800	0.0	68.56561	5
CGTAGTG	3085	0.0	68.31542	4
GGCGCGC	665	0.0	66.348915	6
CGGGGCA	1485	0.0	64.79702	94
GCGTAGT	2055	0.0	64.64012	3
TTCACCG	15675	0.0	64.35118	7
TGTGGCA	6215	0.0	62.685135	8
AGGCGCG	710	0.0	62.1437	5
TGTGTGG	5925	0.0	60.287033	6
CCGCGTA	300	0.0	60.006363	1
CAGCGTA	1300	0.0	59.46332	1
CGGGCCC	930	0.0	59.05144	7
CAGCTGC	135	0.0	58.829765	1
ACCTTCA	6090	0.0	58.57656	4
GCCTTCA	4630	0.0	57.988605	4
GGGCCCG	950	0.0	56.82008	8
>>END_MODULE