FastQCFastQC Report
Tue 7 Mar 2017
1SN_7A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_7A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2964594
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA134190.4526420818499936No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG95790.32311338415985463No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG65420.2206710261168983No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT57160.19280886354084234No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA56230.1896718403936593No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG53180.17938375372816648No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52670.17766345071196932No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48350.16309147222182868No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT47240.1593472833042231No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45060.1519938311957725No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44230.14919412236549084No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC43420.14646187639858949No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA43310.14609083064999795No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG42970.14494396197253318No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA42570.14359470470492755No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA38770.1307767606626742No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT36760.12399674289295601No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30440.10267847806478729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA550.087.9998863
GCTGCGT202.550438E-587.9998863
GCTGCCT158.9719007E-487.9998863
GCAGCCG500.087.9998863
GCAGCAC356.0754246E-1087.999883
AGCTGCC2050.085.853542
CAGCAGC4950.085.333221
CAGCTGC5750.084.939021
AGCAGCG1200.084.333222
CTGCAGC1000.083.599891
AGCAGCA1700.082.823422
AGCTGCA1600.082.4998862
GCAGCAA700.081.714173
CAGCGTA66000.081.3998951
CAGCCCT160600.081.0409851
GCTGCCC1700.080.235183
AGCTGCT1600.079.749892
AGCCCTT135900.079.484812
GCTGCAG455.0931703E-1178.2221153
CAGCACT69900.077.1730041
Produced by FastQC (version 0.11.5)