##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_7A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2964594 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.753855671299341 12.0 12.0 12.0 2.0 27.0 2 14.606436159555068 12.0 12.0 12.0 12.0 22.0 3 13.679779760736208 12.0 12.0 12.0 12.0 22.0 4 13.78283771740751 12.0 12.0 12.0 12.0 22.0 5 22.438508611971823 22.0 22.0 22.0 22.0 27.0 6 26.7816959084448 27.0 22.0 32.0 22.0 32.0 7 34.938344002585175 37.0 37.0 37.0 27.0 41.0 8 37.57841377267849 41.0 37.0 41.0 27.0 41.0 9 38.50975310615889 41.0 37.0 41.0 32.0 41.0 10-11 38.863556864784854 41.0 41.0 41.0 34.5 41.0 12-13 39.04758071425632 41.0 41.0 41.0 37.0 41.0 14-15 39.00313196343243 41.0 41.0 41.0 37.0 41.0 16-17 38.91015076600708 41.0 41.0 41.0 37.0 41.0 18-19 38.85089981967177 41.0 41.0 41.0 37.0 41.0 20-21 38.86194770683608 41.0 41.0 41.0 37.0 41.0 22-23 38.84868720641005 41.0 41.0 41.0 34.5 41.0 24-25 38.86388439698656 41.0 41.0 41.0 37.0 41.0 26-27 38.82946416946132 41.0 41.0 41.0 37.0 41.0 28-29 38.77693303029015 41.0 41.0 41.0 34.5 41.0 30-31 38.64601729612892 41.0 41.0 41.0 32.0 41.0 32-33 38.69425526733171 41.0 41.0 41.0 32.0 41.0 34-35 38.67220064534975 41.0 41.0 41.0 32.0 41.0 36-37 38.59371198889291 41.0 41.0 41.0 32.0 41.0 38-39 38.50083687682023 41.0 41.0 41.0 32.0 41.0 40-41 38.54619806287134 41.0 41.0 41.0 32.0 41.0 42-43 38.2972121983651 41.0 41.0 41.0 32.0 41.0 44-45 38.40915282160053 41.0 41.0 41.0 32.0 41.0 46-47 38.416278586545076 41.0 41.0 41.0 32.0 41.0 48-49 38.35899485730592 41.0 41.0 41.0 32.0 41.0 50-51 38.23637806728341 41.0 41.0 41.0 32.0 41.0 52-53 38.21293354165866 41.0 41.0 41.0 32.0 41.0 54-55 38.085731638126504 41.0 37.0 41.0 32.0 41.0 56-57 37.861246599028405 41.0 37.0 41.0 27.0 41.0 58-59 37.851784089153526 41.0 37.0 41.0 29.5 41.0 60-61 37.8472082517876 41.0 37.0 41.0 27.0 41.0 62-63 37.83414474292264 41.0 37.0 41.0 27.0 41.0 64-65 37.809288725538806 41.0 37.0 41.0 27.0 41.0 66-67 37.70253633381164 41.0 37.0 41.0 27.0 41.0 68-69 37.72114056764603 41.0 37.0 41.0 27.0 41.0 70-71 37.69769587336411 41.0 37.0 41.0 27.0 41.0 72-73 37.5628320437807 41.0 37.0 41.0 27.0 41.0 74-75 37.460032638533306 41.0 37.0 41.0 27.0 41.0 76-77 36.42258990607145 39.0 34.5 41.0 24.5 41.0 78-79 36.937087675411874 41.0 37.0 41.0 27.0 41.0 80-81 37.58803212176777 41.0 37.0 41.0 27.0 41.0 82-83 37.773052060417044 41.0 37.0 41.0 27.0 41.0 84-85 37.6734868248401 41.0 37.0 41.0 27.0 41.0 86-87 37.529061146315485 41.0 37.0 41.0 27.0 41.0 88-89 37.44214992002277 41.0 37.0 41.0 27.0 41.0 90-91 37.276873662970374 41.0 37.0 41.0 27.0 41.0 92-93 37.16576097772578 41.0 37.0 41.0 27.0 41.0 94-95 36.98850112359399 41.0 37.0 41.0 27.0 41.0 96-97 36.92659467029887 41.0 37.0 41.0 27.0 41.0 98-99 36.80431823042211 41.0 37.0 41.0 27.0 41.0 100 36.67314580006571 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.490450656973037 1101 2 -0.6372341830066208 1101 3 0.04906892613172964 1101 4 0.0887488109187462 1101 5 -1.0655489850657283 1101 6 -0.6834474887950996 1101 7 -0.20617900386602628 1101 8 -0.041834798405297136 1101 9 -0.04574361891555867 1101 10-11 -0.015252919855122116 1101 12-13 -0.02325546885859353 1101 14-15 -0.08916676280291114 1101 16-17 -0.015083778800459413 1101 18-19 0.10559521680804096 1101 20-21 0.14387701686587206 1101 22-23 0.13653734625870584 1101 24-25 0.0649778839784716 1101 26-27 0.04827866042993634 1101 28-29 -0.006400089555434363 1101 30-31 -0.140860700178699 1101 32-33 -0.1374536759324556 1101 34-35 -0.03036598369583743 1101 36-37 0.04984374060208552 1101 38-39 0.07556318472661872 1101 40-41 0.0762649856531894 1101 42-43 0.15486183773219153 1101 44-45 -0.010369595859863523 1101 46-47 0.10051089337877528 1101 48-49 -0.032051556059663255 1101 50-51 -0.006284104700590376 1101 52-53 -0.07226391559205325 1101 54-55 -0.1040395040077513 1101 56-57 0.11847086447262711 1101 58-59 0.23618391775926284 1101 60-61 0.062425302321344134 1101 62-63 -0.033513123793483146 1101 64-65 0.10754875076831638 1101 66-67 0.012918795893881452 1101 68-69 -0.0554204660079165 1101 70-71 0.11737472194462839 1101 72-73 0.08621387457398555 1101 74-75 0.12290185443908541 1101 76-77 -0.1570398506287347 1101 78-79 -0.10408184677547894 1101 80-81 -0.018428462820111235 1101 82-83 -0.11330875381122496 1101 84-85 -0.08175521916501793 1101 86-87 -0.10039577868342775 1101 88-89 -0.24023284482133533 1101 90-91 -0.15817414534175356 1101 92-93 -8.734487377068945E-4 1101 94-95 -0.11203529728600614 1101 96-97 -0.08216795984584735 1101 98-99 -0.1815596859212505 1101 100 -0.3233786934251981 1102 1 1.660895549195443 1102 2 0.8326293173206984 1102 3 -0.10074329622646339 1102 4 -0.060138186266668114 1102 5 -0.7249888956263071 1102 6 0.059106750488624016 1102 7 -0.032432409229308234 1102 8 -0.3120171821180122 1102 9 -0.3080840138604728 1102 10-11 -0.4130669231603932 1102 12-13 -0.33803860677090825 1102 14-15 -0.2778988865021077 1102 16-17 -0.2968763275745303 1102 18-19 -0.244669489668361 1102 20-21 -0.20830660364864428 1102 22-23 -0.2297496464119746 1102 24-25 -0.12561256116325126 1102 26-27 -0.1166619588263913 1102 28-29 -0.23508367383320206 1102 30-31 -0.3252894129899957 1102 32-33 -0.3887344370705037 1102 34-35 -0.22023778283256945 1102 36-37 -0.24387931202911517 1102 38-39 -0.2746746412110497 1102 40-41 -0.3510700866480363 1102 42-43 -0.1416527811225592 1102 44-45 -0.21773720994918477 1102 46-47 -0.40024933578004607 1102 48-49 -0.5047689973412801 1102 50-51 -0.3492463638301757 1102 52-53 -0.43494817351690784 1102 54-55 -0.2254728574990139 1102 56-57 -0.21719949160986118 1102 58-59 -0.13848638723534634 1102 60-61 -0.16635006191737745 1102 62-63 -0.2884710238204491 1102 64-65 -0.20589644916942973 1102 66-67 -0.12027768926034099 1102 68-69 -0.433513064562959 1102 70-71 -0.4539451392389253 1102 72-73 -0.2105373227238232 1102 74-75 -0.5475176423572279 1102 76-77 -0.32493732914509366 1102 78-79 -0.4702770594851913 1102 80-81 -0.5408884152727467 1102 82-83 -0.3090871429273676 1102 84-85 -0.21923863045686431 1102 86-87 -0.20986283710301024 1102 88-89 -0.38428164205831195 1102 90-91 -0.5173392803622505 1102 92-93 -0.29552662741338764 1102 94-95 -0.18492224932399637 1102 96-97 -0.20531721247776602 1102 98-99 -0.5067797499117788 1102 100 -0.5868474769516183 1103 1 1.7922648686992453 1103 2 0.7460090212185193 1103 3 0.5412400250652194 1103 4 1.0287598189010385 1103 5 2.881573600546897 1103 6 -0.2353281694854168 1103 7 -0.5217528926914241 1103 8 -0.2782342257496069 1103 9 -0.17918833538482204 1103 10-11 -0.23264297537625822 1103 12-13 -0.3578457974739351 1103 14-15 -0.3844396464732043 1103 16-17 -0.5405125186571809 1103 18-19 -0.35957685058728117 1103 20-21 -0.2277908127570356 1103 22-23 -0.2135399502289701 1103 24-25 -0.30028651813169205 1103 26-27 -0.08473851924079412 1103 28-29 -0.22218737082442175 1103 30-31 -0.1354460361026213 1103 32-33 -0.41911185101351833 1103 34-35 -0.41405582326802914 1103 36-37 -0.14808970621973572 1103 38-39 -0.048232704423597283 1103 40-41 -0.31895285810565355 1103 42-43 -0.17977165059513567 1103 44-45 -0.36203222225616116 1103 46-47 -0.4495191830755729 1103 48-49 -0.3168827970743209 1103 50-51 -0.27054049444331696 1103 52-53 -0.21991253839120617 1103 54-55 -0.4566967587493025 1103 56-57 -0.45722961614911384 1103 58-59 -0.5106567010938647 1103 60-61 -0.3035110077789298 1103 62-63 -0.24218852666759005 1103 64-65 -0.21282203176087933 1103 66-67 -0.5192392498584226 1103 68-69 -0.23654358096613493 1103 70-71 -0.6844560318314734 1103 72-73 -0.39293742803724996 1103 74-75 -0.539010827598652 1103 76-77 -0.5343500230871214 1103 78-79 -0.6067660199436773 1103 80-81 -0.29993159377900724 1103 82-83 -0.2681125650995213 1103 84-85 -0.34693300108666136 1103 86-87 -0.17008803640255366 1103 88-89 -0.16445822573962232 1103 90-91 -0.6623616900804734 1103 92-93 -0.2331666881522949 1103 94-95 -0.3750726883340505 1103 96-97 -0.40721683408416 1103 98-99 -0.40910460510581714 1103 100 -0.3411983910966825 1104 1 1.6031732760378148 1104 2 -0.6598332741010342 1104 3 0.039972663269809416 1104 4 0.09442689690442663 1104 5 -1.0591090162823313 1104 6 -0.39071976305643474 1104 7 -0.1636432931838172 1104 8 0.05075455294416287 1104 9 0.029999886033586165 1104 10-11 -0.034034875175848356 1104 12-13 -0.11156025904224975 1104 14-15 -0.10746379001860618 1104 16-17 -0.039411005402939736 1104 18-19 -0.015154455273481915 1104 20-21 0.06372900568884177 1104 22-23 0.12990433284750225 1104 24-25 -0.07236847823708104 1104 26-27 -0.1505609097034366 1104 28-29 -0.08509589034573395 1104 30-31 -0.02820448846588164 1104 32-33 0.02262922515250665 1104 34-35 -0.10822216905162918 1104 36-37 -0.09830436941378906 1104 38-39 -0.02253952119279745 1104 40-41 -0.0939458087846603 1104 42-43 -0.006171357707216885 1104 44-45 -0.035865900728289546 1104 46-47 -0.3803329021154127 1104 48-49 -0.342036441952267 1104 50-51 -0.19822863551463143 1104 52-53 -0.1659029149310669 1104 54-55 -0.2507879117756602 1104 56-57 -0.15900839481255957 1104 58-59 -0.2011450447005103 1104 60-61 -0.1845956329824503 1104 62-63 -0.26491546174409564 1104 64-65 -0.2920201677081664 1104 66-67 -0.49919977366086954 1104 68-69 -0.4567617915604316 1104 70-71 -0.14853936238550602 1104 72-73 -0.12900205742489135 1104 74-75 -0.36874986628350115 1104 76-77 -0.2877465072193246 1104 78-79 -0.2215579967552017 1104 80-81 -0.23368609245859062 1104 82-83 -0.2930636746474775 1104 84-85 -0.20535731866690554 1104 86-87 -0.29817025725701995 1104 88-89 -0.16839295963913514 1104 90-91 -0.2758536356627488 1104 92-93 -0.3840104084958824 1104 94-95 -0.2235960450842711 1104 96-97 -0.28606993229863065 1104 98-99 -0.3208364944147988 1104 100 -0.18342185305611736 1105 1 1.4442711967302007 1105 2 0.3426225640452998 1105 3 0.009548837778917019 1105 4 -0.2217595119126905 1105 5 -1.2187473038692147 1105 6 0.3781128924710515 1105 7 0.03526888017789531 1105 8 -0.22892106326510486 1105 9 -0.10647977002869169 1105 10-11 -0.25113543006990113 1105 12-13 -0.19864283518695913 1105 14-15 -0.12482046905719102 1105 16-17 -0.14393679992434727 1105 18-19 -0.2595367750591677 1105 20-21 -0.14375760860598774 1105 22-23 -0.12816991951940793 1105 24-25 -0.23586007848963675 1105 26-27 -0.17033812118723546 1105 28-29 -0.2286035829515498 1105 30-31 -0.32443532227791394 1105 32-33 -0.3628803315356066 1105 34-35 -0.16329333756463882 1105 36-37 -0.1476999079182164 1105 38-39 -0.17044985674962732 1105 40-41 -0.131164036626366 1105 42-43 -0.00534156362973448 1105 44-45 -0.1436036332449575 1105 46-47 -0.28170763712080316 1105 48-49 -0.2064855240113772 1105 50-51 -0.1910253182760968 1105 52-53 -0.2242545042234454 1105 54-55 -0.1377245234354092 1105 56-57 0.05485900930530363 1105 58-59 -0.03522982917645834 1105 60-61 -0.2831792064214653 1105 62-63 -0.48469117585511867 1105 64-65 -0.2609605459944433 1105 66-67 -0.34204807908263746 1105 68-69 -0.3843437796927276 1105 70-71 -0.4093232722636273 1105 72-73 -0.41158800845698096 1105 74-75 -0.3423669654584671 1105 76-77 -0.3496904699517742 1105 78-79 -0.3237335032305353 1105 80-81 -0.4611853874455747 1105 82-83 -0.34672709255350753 1105 84-85 -0.28571155302424955 1105 86-87 -0.26551747495491185 1105 88-89 -0.31441800011590715 1105 90-91 -0.3798928574340792 1105 92-93 -0.3126659541847516 1105 94-95 -0.19299282846331778 1105 96-97 -0.15428336546465005 1105 98-99 -0.3476089323622773 1105 100 -0.33244391449508726 1106 1 1.619447030052612 1106 2 -0.6550080267543752 1106 3 -0.15502435386323654 1106 4 -0.23988259012537405 1106 5 -1.122846795146934 1106 6 -0.5145987149551061 1106 7 -0.22331416098207768 1106 8 -0.1611465406864383 1106 9 -0.1750988744291746 1106 10-11 -0.07757818653492876 1106 12-13 -0.09852489043016277 1106 14-15 -0.043535517895016085 1106 16-17 -0.062304236513369915 1106 18-19 0.020593688635550222 1106 20-21 0.00794932092572509 1106 22-23 -0.05953273957004512 1106 24-25 -0.2182364183307044 1106 26-27 -0.13219151163449538 1106 28-29 -0.15856126312264252 1106 30-31 -0.07025025659886097 1106 32-33 -0.1526429023597089 1106 34-35 -0.16896704351736958 1106 36-37 -0.3344821159898217 1106 38-39 -0.06958030042441976 1106 40-41 -0.035899988864287025 1106 42-43 2.1944019550801386E-4 1106 44-45 -0.10233888348905396 1106 46-47 -0.276838329100336 1106 48-49 -0.16614061085250853 1106 50-51 -0.20603551331775094 1106 52-53 -0.15722561603965346 1106 54-55 -0.3549513568829781 1106 56-57 -0.1677598107833589 1106 58-59 -0.2736105278893177 1106 60-61 -0.28030973104014834 1106 62-63 -0.2986631718609303 1106 64-65 -0.45372035968522084 1106 66-67 -0.3460736694509521 1106 68-69 -0.33779369623553634 1106 70-71 -0.4692770593503752 1106 72-73 -0.4412735327940851 1106 74-75 -0.4614656048089216 1106 76-77 -0.5126012151958577 1106 78-79 -0.3453166447782081 1106 80-81 -0.31538282515370497 1106 82-83 -0.4583735647752789 1106 84-85 -0.546156159676535 1106 86-87 -0.4966037175034259 1106 88-89 -0.43963157604854786 1106 90-91 -0.505849043167764 1106 92-93 -0.38123767328180236 1106 94-95 -0.462144592644087 1106 96-97 -0.48285572404724064 1106 98-99 -0.6716139382091058 1106 100 -0.6366816153083121 1107 1 1.3397058135811335 1107 2 -0.5505385257227289 1107 3 -0.052400192984448424 1107 4 -0.21930196183790862 1107 5 -1.3468485678359023 1107 6 -0.23310224580280092 1107 7 -0.30954211134678644 1107 8 -0.2922893438598422 1107 9 -0.3594669544036009 1107 10-11 -0.21691008621817076 1107 12-13 -0.31569861394880405 1107 14-15 -0.42117798687296926 1107 16-17 -0.39310942962022466 1107 18-19 -0.2724554090367022 1107 20-21 -0.23578596720888356 1107 22-23 -0.16370197324532398 1107 24-25 -0.14519414987003643 1107 26-27 -0.4123157451644701 1107 28-29 -0.30762195592436825 1107 30-31 -0.3941074534254554 1107 32-33 -0.1908925899596099 1107 34-35 -0.34924813096033347 1107 36-37 -0.32982350391809945 1107 38-39 -0.42787837371493964 1107 40-41 -0.43388471758475333 1107 42-43 -0.3228425167368627 1107 44-45 -0.12071483885215883 1107 46-47 -0.436330206697626 1107 48-49 -0.36107268199254605 1107 50-51 -0.48710166948874445 1107 52-53 -0.4265267116195517 1107 54-55 -0.46712377268025307 1107 56-57 -0.30980835630658987 1107 58-59 -0.5497754170236036 1107 60-61 -0.39612306107995465 1107 62-63 -0.5149807737905476 1107 64-65 -0.5562744848196246 1107 66-67 -0.6171268937106262 1107 68-69 -0.7112831327529321 1107 70-71 -0.45031611662856363 1107 72-73 -0.29411550193186287 1107 74-75 -0.48404414074219915 1107 76-77 -0.6371316705734955 1107 78-79 -0.8423792846317397 1107 80-81 -0.5567880444149864 1107 82-83 -0.7086077195513667 1107 84-85 -0.36494026940888347 1107 86-87 -0.42535302731715063 1107 88-89 -0.42911146619995577 1107 90-91 -0.32546435513196315 1107 92-93 -0.337798729339454 1107 94-95 -0.44927922982406443 1107 96-97 -0.2991020551622938 1107 98-99 -0.5833020611930309 1107 100 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0.2685848928132515 2223 26-27 0.22417846085660642 2223 28-29 0.10471616205152401 2223 30-31 0.15263743680921493 2223 32-33 0.13831779844078795 2223 34-35 0.21084955144809214 2223 36-37 0.23771143464825428 2223 38-39 0.14373025611094903 2223 40-41 0.2031331301995607 2223 42-43 0.3961090351177958 2223 44-45 0.47582653423349797 2223 46-47 0.43140257150071903 2223 48-49 0.31658832656740543 2223 50-51 0.35322922785010036 2223 52-53 0.41932491593532717 2223 54-55 0.41950028834577324 2223 56-57 0.4077219237842513 2223 58-59 0.5526079755111937 2223 60-61 0.26579433832608146 2223 62-63 0.31928318707825554 2223 64-65 0.44154593094931016 2223 66-67 0.4874930038029035 2223 68-69 0.5035590613890335 2223 70-71 0.5025607831559782 2223 72-73 0.5769311992851627 2223 74-75 0.46850346305445356 2223 76-77 0.5404058883853367 2223 78-79 0.506666746929433 2223 80-81 0.45424875754157057 2223 82-83 0.3872096973239607 2223 84-85 0.37157868476335665 2223 86-87 0.37513480241556607 2223 88-89 0.4805390025591407 2223 90-91 0.4876520194094738 2223 92-93 0.49723693711369776 2223 94-95 0.683805845585006 2223 96-97 0.5257193214172204 2223 98-99 0.5784227393659833 2223 100 0.6071531917533406 2224 1 1.1912594858975183 2224 2 -0.9546671654445884 2224 3 -0.07299993222825485 2224 4 -0.22764616001261473 2224 5 -1.0718051084704072 2224 6 0.5768535705089484 2224 7 0.375648385538625 2224 8 0.08389709681812008 2224 9 0.09847452031753079 2224 10-11 0.13795736271707426 2224 12-13 0.19772236243827734 2224 14-15 0.1129253015523588 2224 16-17 0.133254107972121 2224 18-19 0.06375529645366385 2224 20-21 0.09350202351978965 2224 22-23 0.24266880757460285 2224 24-25 0.21299105945924168 2224 26-27 0.22707509939586146 2224 28-29 0.09965857427989278 2224 30-31 0.1509961247289695 2224 32-33 0.07020113740529865 2224 34-35 0.1651343838882795 2224 36-37 0.040102977321943456 2224 38-39 0.0365189897502205 2224 40-41 0.08310535919346052 2224 42-43 0.42835334013695814 2224 44-45 0.2944894201656538 2224 46-47 0.24497864452648344 2224 48-49 0.13691333857821775 2224 50-51 0.20597054549025273 2224 52-53 0.01400059534842768 2224 54-55 0.238923598028137 2224 56-57 0.32358618229980607 2224 58-59 0.28172248244040077 2224 60-61 0.3311813717753225 2224 62-63 0.31388566109284 2224 64-65 0.34180895716211523 2224 66-67 0.3716841304817464 2224 68-69 0.43076887362110483 2224 70-71 0.3102886928745505 2224 72-73 0.37437428511724846 2224 74-75 0.37414070642569897 2224 76-77 0.47919854817197916 2224 78-79 0.28398497728336736 2224 80-81 0.43200450954705616 2224 82-83 0.24560023351251914 2224 84-85 0.06192341304801374 2224 86-87 0.21988853432271327 2224 88-89 0.38027611890797175 2224 90-91 0.48318007482603065 2224 92-93 0.3350380807431179 2224 94-95 0.5841221898485429 2224 96-97 0.33015804914018076 2224 98-99 0.07699056520128522 2224 100 0.6712888191884758 2225 1 1.6787043243778577 2225 2 1.5933070553529358 2225 3 0.8689526909149734 2225 4 1.1465666446114877 2225 5 3.2151609974312976 2225 6 0.0603466917079416 2225 7 0.21486421203943706 2225 8 0.2768208571825994 2225 9 0.1864125346851182 2225 10-11 0.1277516928069744 2225 12-13 0.18576968320453346 2225 14-15 0.2421930538510182 2225 16-17 0.32354315643853937 2225 18-19 0.15083701893740198 2225 20-21 0.14107267303397464 2225 22-23 0.2593410131800198 2225 24-25 0.2358518722389107 2225 26-27 0.2825404894423187 2225 28-29 0.18376343270040962 2225 30-31 0.16490222639583862 2225 32-33 0.17248675593648954 2225 34-35 0.219227869956093 2225 36-37 0.1291679904401093 2225 38-39 0.26935543119942906 2225 40-41 0.2568405530493081 2225 42-43 0.3746667907836141 2225 44-45 0.27506183793344974 2225 46-47 0.2675603368615711 2225 48-49 0.2909387447787424 2225 50-51 0.3547969214958471 2225 52-53 0.3428031469061352 2225 54-55 0.386638470288986 2225 56-57 0.33514510609101933 2225 58-59 0.38950174661692927 2225 60-61 0.3764682958133321 2225 62-63 0.2795604370462712 2225 64-65 0.30782483358348145 2225 66-67 0.3034391467269657 2225 68-69 0.32417164701681145 2225 70-71 0.3922100876213932 2225 72-73 0.367864279269817 2225 74-75 0.41127059664253096 2225 76-77 0.5112449546627715 2225 78-79 0.6325564218806576 2225 80-81 0.34483318245940353 2225 82-83 0.25348733171158955 2225 84-85 0.23972895563263563 2225 86-87 0.2798152240145413 2225 88-89 0.3884744493736747 2225 90-91 0.4510115312303711 2225 92-93 0.3115570470509397 2225 94-95 0.5585969371958086 2225 96-97 0.5531416300430436 2225 98-99 0.4173079393797323 2225 100 0.5477375321214026 2226 1 1.4829893956706872 2226 2 1.531809350281863 2226 3 0.8373529643060227 2226 4 1.0636199669795428 2226 5 3.0199711795808604 2226 6 -0.2937222228406853 2226 7 -0.08403694487147106 2226 8 0.047785283005829626 2226 9 0.12453419370202568 2226 10-11 0.148058340877121 2226 12-13 0.06200230453570299 2226 14-15 -0.007280168273645415 2226 16-17 -0.0839532872551274 2226 18-19 0.015747805659053427 2226 20-21 0.10336443462809086 2226 22-23 -0.06452860929584148 2226 24-25 -0.07308737604684268 2226 26-27 -0.1128984087695244 2226 28-29 -0.022673620677522877 2226 30-31 -0.04205521461948791 2226 32-33 0.0078272722677184 2226 34-35 -0.042973733715790274 2226 36-37 0.03131886117948568 2226 38-39 -0.07317688117762344 2226 40-41 0.07075362914314809 2226 42-43 0.13137390117358194 2226 44-45 0.01874842176781044 2226 46-47 -0.004427221622997024 2226 48-49 0.017164975871970967 2226 50-51 0.007206596871760951 2226 52-53 0.24589866151477935 2226 54-55 0.19382915498908915 2226 56-57 0.25067671832027827 2226 58-59 0.14413675289603134 2226 60-61 0.14747912032073174 2226 62-63 0.100122555715771 2226 64-65 0.19876569887726703 2226 66-67 0.11725886977961153 2226 68-69 0.1865584286707218 2226 70-71 0.1640323334288425 2226 72-73 0.15651021563415668 2226 74-75 0.19884908543460966 2226 76-77 0.18236316295119082 2226 78-79 0.05956962466790827 2226 80-81 0.17304261000521848 2226 82-83 0.16789342855425815 2226 84-85 0.09905800843267087 2226 86-87 0.20754593938778498 2226 88-89 0.2543615533178425 2226 90-91 0.3136372522159192 2226 92-93 0.07177073105090415 2226 94-95 0.30215877247214706 2226 96-97 0.23720965551508755 2226 98-99 0.15135637738331553 2226 100 0.20533469152172046 2227 1 1.2729578765968874 2227 2 1.8179709093283432 2227 3 0.6386610338607248 2227 4 -0.1309991168959037 2227 5 3.1787660088852014 2227 6 0.2527639322801818 2227 7 0.20141697457854235 2227 8 0.4498449397110491 2227 9 0.45831306752495493 2227 10-11 0.5016224315266484 2227 12-13 0.3713828679601434 2227 14-15 0.344108238529536 2227 16-17 0.31405269745000197 2227 18-19 0.23255858484638026 2227 20-21 0.26515609522980554 2227 22-23 0.2266195975319789 2227 24-25 0.399133710941328 2227 26-27 0.38922185077297655 2227 28-29 0.16975830260180658 2227 30-31 0.2913764175422884 2227 32-33 0.2939703565462466 2227 34-35 0.3327558796576966 2227 36-37 0.38131144328023936 2227 38-39 0.27274803288671023 2227 40-41 0.2680771671171911 2227 42-43 0.5634328020928407 2227 44-45 0.356119658233645 2227 46-47 0.47576656749593127 2227 48-49 0.3615017596947965 2227 50-51 0.4793379692670996 2227 52-53 0.389684841268668 2227 54-55 0.47599960431784183 2227 56-57 0.5961162498449397 2227 58-59 0.5724497546539311 2227 60-61 0.41073812537050003 2227 62-63 0.4676780639958267 2227 64-65 0.37418082131459585 2227 66-67 0.4921573985162766 2227 68-69 0.3411090999206152 2227 70-71 0.39937890328025816 2227 72-73 0.4286558639865419 2227 74-75 0.43597721074952034 2227 76-77 0.5746943147689905 2227 78-79 0.6127957110402491 2227 80-81 0.35427462509854735 2227 82-83 0.26262504056173697 2227 84-85 0.27961625433743365 2227 86-87 0.3966798366680777 2227 88-89 0.3348078309320215 2227 90-91 0.330965184945363 2227 92-93 0.41811202719583207 2227 94-95 0.507328768329323 2227 96-97 0.5958426906395431 2227 98-99 0.672996628864091 2227 100 0.5239320647456225 2228 1 1.334980146538669 2228 2 1.4918197548542675 2228 3 -0.06723556430234545 2228 4 -0.06209096539354064 2228 5 3.15548933289546 2228 6 0.1645295005042584 2228 7 0.3437394774139477 2228 8 0.6400687921792425 2228 9 0.21961754245992182 2228 10-11 0.2636683545068266 2228 12-13 0.25056898354202417 2228 14-15 0.2568049865609723 2228 16-17 0.3088393027822818 2228 18-19 0.2556236098399509 2228 20-21 0.11016624855731294 2228 22-23 0.29079756423335823 2228 24-25 0.36184275065897253 2228 26-27 0.3330912735965441 2228 28-29 0.17096858262389958 2228 30-31 0.254426932143879 2228 32-33 0.27929588747802114 2228 34-35 0.2801380887951055 2228 36-37 0.2782728595080286 2228 38-39 0.18998896938916232 2228 40-41 0.18673383241091557 2228 42-43 0.49193952009139963 2228 44-45 0.3601802844386768 2228 46-47 0.36262695226517394 2228 48-49 0.4349734111476806 2228 50-51 0.25235725283658184 2228 52-53 0.4287607742880013 2228 54-55 0.2938335019128715 2228 56-57 0.31323091464417985 2228 58-59 0.4631297950135149 2228 60-61 0.42361380765071033 2228 62-63 0.3898674614015505 2228 64-65 0.301626535618027 2228 66-67 0.49252354134848275 2228 68-69 0.5035474688138564 2228 70-71 0.542910981339233 2228 72-73 0.530665089517278 2228 74-75 0.5426910368948938 2228 76-77 0.5521393524658436 2228 78-79 0.5535062268879258 2228 80-81 0.4962193559261223 2228 82-83 0.417317250818364 2228 84-85 0.449584056321747 2228 86-87 0.5233613802219494 2228 88-89 0.49319297400471385 2228 90-91 0.4749012535171886 2228 92-93 0.5125978104229247 2228 94-95 0.751202659575533 2228 96-97 0.4115318158520722 2228 98-99 0.6909672996603149 2228 100 0.6862825452631611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 25.0 13 39.0 14 149.0 15 490.0 16 2053.0 17 5109.0 18 8273.0 19 10645.0 20 12430.0 21 14392.0 22 16402.0 23 18923.0 24 21219.0 25 24167.0 26 27505.0 27 30589.0 28 34465.0 29 38855.0 30 44391.0 31 51313.0 32 60110.0 33 71726.0 34 87435.0 35 112976.0 36 159019.0 37 261918.0 38 646670.0 39 1201967.0 40 1339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.538199427067592 19.21557895025324 18.426597888387043 36.81962373429213 2 18.467561162788737 50.72673452693187 13.207451698052742 17.598252612226652 3 41.54403604675716 16.473453025945542 22.734546450542638 19.24796447675466 4 17.08513880821455 16.190952285540618 22.041736575058845 44.682172331185996 5 17.350267861299052 34.542571427993174 26.47428281916512 21.632877891542655 6 17.678643348802566 29.036893416096778 29.11302525742142 24.171437977679236 7 20.293200350537038 26.136496262220057 35.687146368103015 17.88315701913989 8 21.942667360184902 28.479886284597487 32.445623245543906 17.131823109673704 9 21.157197241848294 28.388035596105233 27.071160502922154 23.383606659124318 10-11 19.80944102295289 30.298954932783378 28.372923914708053 21.518680129555683 12-13 24.237855166677125 26.703858943248214 27.266904001020038 21.791381889054623 14-15 21.822600329083848 27.604656826533414 28.44760530447002 22.125137539912718 16-17 20.728217759328935 29.7063274094193 28.33509411406756 21.230360717184208 18-19 21.448501885924344 29.387531648515786 29.735656889273876 19.428309576285994 20-21 22.68575393460285 28.908865767116847 29.86331349250521 18.54206680577509 22-23 21.62491727366378 30.405917302672812 28.64805433728868 19.321111086374728 24-25 22.18636008843032 28.32753827336897 26.747153235822513 22.7389484023782 26-27 20.08060800230993 26.856392477351033 29.969820488066834 23.09317903227221 28-29 19.73621540738682 28.150380931113972 30.436153138827855 21.677250522671354 30-31 20.209431431210923 29.907922969633926 28.300551409661235 21.582094189493915 32-33 20.74763019826661 29.94954789762106 28.68753697808199 20.615284926030345 34-35 21.454219363595826 29.121576175354875 29.932547256049226 19.491657205000077 36-37 21.721338571150046 28.154243043060873 28.848520235823184 21.275898149965897 38-39 21.58107015929471 29.271684778355567 29.08624460629677 20.061000456052955 40-41 20.986770003282114 29.696773642956387 29.76140400350677 19.555052350254726 42-43 20.505857129750062 29.827999940632637 29.311723164604103 20.354419765013194 44-45 20.439088792596895 30.23602894696542 28.75218326691614 20.57269899352154 46-47 19.96324547576296 28.171114978311927 28.906003070298087 22.959636475627022 48-49 21.0369686496595 28.23412859707892 29.997659037851065 20.731243715410514 50-51 21.79885340117399 30.746436105584777 28.111640244836224 19.34307024840501 52-53 20.328522146201667 30.292381741708436 28.24174941955758 21.137346692532322 54-55 22.32484864556569 28.526901185278682 28.08875816404697 21.05949200510866 56-57 21.370911765812203 28.653571632790513 29.340506808881223 20.63500979251606 58-59 21.24655401677283 28.045272498139852 29.035509293675283 21.672664191412043 60-61 19.710661361602124 30.486895567462398 29.373486404605565 20.428956666329913 62-63 20.472052589427463 31.11435420676961 27.36162923050246 21.05196397330046 64-65 19.830850162948305 30.305838192492352 28.689291151678454 21.17402049288089 66-67 20.30035141405535 30.294266263778447 29.315616236152405 20.089766086013803 68-69 20.94785997677928 29.94664699449571 29.22774585659959 19.877747172125424 70-71 19.564685754609233 30.9348598830059 29.312580407300292 20.187873955084576 72-73 20.105957173225068 30.772105725100975 28.575076384827064 20.546860716846894 74-75 20.060031828978943 29.326258502850642 30.176931478644292 20.43677818952612 76-77 19.51768100454902 30.64453345044886 28.649892700315792 21.18789284468632 78-79 19.588601339677542 29.468689473162264 28.528965517706638 22.413743669453556 80-81 20.67994225171103 29.648821590843927 28.404315605193297 21.266920552251744 82-83 20.489213700088442 28.39515292819185 29.640213803306626 21.47541956841308 84-85 20.68084196352013 29.110647191487267 28.293553855941152 21.91495698905145 86-87 21.433929907434205 28.37973766390946 30.04139858611331 20.144933842543026 88-89 21.467796264851106 27.64100244417954 30.379842905976336 20.51135838499302 90-91 22.135054580829618 27.57203853208905 30.269945901529855 20.022960985551478 92-93 22.785615838121508 27.7735332392901 30.361830321453798 19.07902060113459 94-95 22.825267136073272 27.86508034489714 29.86908156732423 19.44057095170536 96-97 21.934234502262367 28.106580530082702 30.217830839568588 19.741354128086343 98-99 23.24699436077925 28.414868950014743 29.300504554755218 19.03763213445079 100 22.448368984083487 29.66794104015592 28.352347741377066 19.531342234383526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 10.5 2 18.0 3 19.5 4 25.5 5 37.5 6 43.0 7 42.5 8 55.0 9 74.0 10 82.5 11 98.0 12 113.5 13 143.0 14 172.5 15 212.0 16 280.0 17 349.5 18 513.5 19 835.0 20 1324.5 21 1997.5 22 3005.5 23 4772.5 24 7397.5 25 10912.5 26 15570.5 27 20996.0 28 27087.0 29 34240.5 30 42911.0 31 52686.0 32 63219.0 33 75156.5 34 88073.0 35 101895.0 36 115503.5 37 125807.5 38 133963.5 39 140267.0 40 143679.0 41 144695.0 42 142844.0 43 140091.5 44 137628.0 45 133570.5 46 124871.0 47 114577.5 48 107182.5 49 102771.5 50 99051.5 51 91735.5 52 80537.5 53 68654.5 54 57494.5 55 46468.0 56 35744.5 57 26504.0 58 19259.5 59 14000.5 60 11104.5 61 9428.0 62 7654.0 63 5806.0 64 4271.5 65 3569.5 66 3632.0 67 3363.5 68 2492.0 69 2268.5 70 2837.5 71 2789.5 72 1712.5 73 739.0 74 363.0 75 227.0 76 155.5 77 106.5 78 85.0 79 70.5 80 50.0 81 38.5 82 30.5 83 23.5 84 22.0 85 23.5 86 21.5 87 18.0 88 18.5 89 17.5 90 15.5 91 13.0 92 10.5 93 10.5 94 15.5 95 17.0 96 12.5 97 21.5 98 43.0 99 85.0 100 97.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.381254903706882 2 0.005700611955633723 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 5.39702907042246E-4 30-31 0.009529129452464653 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.770172239436497E-4 40-41 0.001450451562676036 42-43 6.746286338028075E-5 44-45 0.0 46-47 0.0020576173330985625 48-49 3.3731431690140374E-5 50-51 0.0 52-53 0.0015179144260563165 54-55 0.020491844751760278 56-57 0.011485552490492797 58-59 0.015330935703168798 60-61 1.8552287429577204E-4 62-63 0.011738538228168848 64-65 1.1806001091549128E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.349257267605615E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2964594.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.66723826172465 #Duplication Level Percentage of deduplicated Percentage of total 1 85.78213050857015 63.19332646773197 2 8.82377553906955 13.000463500092287 3 2.4198705414003507 5.347955392076049 4 1.051382196222788 3.098096910131179 5 0.5672695119852086 2.089458914901331 6 0.3361683512103682 1.4858756414799197 7 0.22211806464026954 1.1453977073061525 8 0.15725305576905782 0.9267518661378766 9 0.1080450115596209 0.7163439848598048 >10 0.49719781152516956 6.186015460626844 >50 0.02389433387260076 1.1864396884232795 >100 0.010382201681857049 1.3574433759111972 >500 4.6624772096612505E-4 0.23095045139503767 >1k 4.662477206161439E-5 0.03548063892709481 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 1.6865715845070183E-5 0.0 0.0 0.0 24-25 0.0 3.373143169014037E-5 0.0 0.0 0.0 26-27 0.0 3.373143169014037E-5 0.0 0.0 0.0 28-29 0.0 3.373143169014037E-5 0.0 0.0 0.0 30-31 0.0 3.373143169014037E-5 0.0 0.0 0.0 32-33 0.0 3.373143169014037E-5 0.0 0.0 0.0 34-35 0.0 3.373143169014037E-5 0.0 0.0 0.0 36-37 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 0.0 38-39 3.373143169014037E-5 3.373143169014037E-5 1.6865715845070183E-5 0.0 0.0 40-41 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 42-43 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 44-45 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 46-47 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 48-49 6.746286338028073E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 0.0 50-51 6.746286338028073E-5 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 52-53 6.746286338028073E-5 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 54-55 6.746286338028073E-5 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 56-57 6.746286338028073E-5 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 58-59 1.0119429507042111E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 60-61 1.3492572676056147E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 62-63 1.6865715845070184E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 64-65 1.6865715845070184E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 66-67 2.192543059859124E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 68-69 3.035828852112633E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 70-71 3.541800327464739E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 72-73 4.722400436619652E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 74-75 5.734343387323864E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 76-77 6.746286338028074E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 78-79 6.914943496478776E-4 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 80-81 0.0010625400982394216 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 82-83 0.0012986601200704042 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 84-85 0.0013829886992957551 3.373143169014037E-5 3.373143169014037E-5 3.373143169014037E-5 0.0 86-87 0.0014167201309858955 1.0119429507042111E-4 3.373143169014037E-5 3.373143169014037E-5 0.0 88 0.0014167201309858955 1.0119429507042111E-4 3.373143169014037E-5 3.373143169014037E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11545 0.0 81.02997 8 CACCGGC 11335 0.0 79.71527 9 CTTCACC 12895 0.0 74.33044 6 CCTTCAC 14190 0.0 70.59019 5 CGGGCCC 885 0.0 70.54085 7 TTCACCG 13700 0.0 69.3804 7 CGTAGTG 2535 0.0 67.21419 4 GTGTGGC 4030 0.0 67.20523 7 CGGGGCA 1245 0.0 65.22424 94 AACGGGC 965 0.0 64.20649 5 GGGCCCG 995 0.0 62.27062 8 ACGGGCC 1020 0.0 61.204556 6 ACCTTCA 5605 0.0 60.54733 4 CAGCGTA 1145 0.0 59.69404 1 GCCTTCA 4285 0.0 59.48142 4 GGCTGCG 45 7.939076E-6 58.87138 1 GGCCCGA 1045 0.0 58.39281 9 CGCAGCC 55 3.8540202E-7 57.800995 1 GCGTAGT 1760 0.0 56.806656 3 TGTGGCA 4975 0.0 55.85486 8 >>END_MODULE