FastQCFastQC Report
Tue 7 Mar 2017
1SN_7A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_7A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2964594
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG115450.081.029978
CACCGGC113350.079.715279
CTTCACC128950.074.330446
CCTTCAC141900.070.590195
CGGGCCC8850.070.540857
TTCACCG137000.069.38047
CGTAGTG25350.067.214194
GTGTGGC40300.067.205237
CGGGGCA12450.065.2242494
AACGGGC9650.064.206495
GGGCCCG9950.062.270628
ACGGGCC10200.061.2045566
ACCTTCA56050.060.547334
CAGCGTA11450.059.694041
GCCTTCA42850.059.481424
GGCTGCG457.939076E-658.871381
GGCCCGA10450.058.392819
CGCAGCC553.8540202E-757.8009951
GCGTAGT17600.056.8066563
TGTGGCA49750.055.854868
Produced by FastQC (version 0.11.5)