FastQCFastQC Report
Tue 7 Mar 2017
1SN_8A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_8A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3269810
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA165400.5058397888562333No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA150340.45978206684792083No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG106370.32530942164835264No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG89860.27481719121294507No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG70830.21661809095941353No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66570.20358981102877538No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG66000.2018465904746759No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT64090.1960052724776057No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61390.18774791195818719No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG58360.17848131848639523No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG51890.15869423605652927No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC50200.15352574002770802No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45840.14019163192968398No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA45160.13811200039146007No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44450.13594062040302035No Hit
CTGCGGACGAGCCTAAGAGGTAACCCTAGCCTTAGCTGTATCCCAAAGCG43720.1337080747811035No Hit
CTGCAACCCAGGGTTAGGGCTACCCCAAGAGTTAGGGTTACGCTTTGGGA43180.13205660267721978No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT41790.12780559115055615No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41610.12725510044926158No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC41410.12664344411448983No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA41090.12566479397885505No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA38960.11915065401353596No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA36740.11236126869756959No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34050.10413449099488963No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA33260.10171844847254122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCG158.9719816E-487.999893
GCTGCCC1000.087.999893
GCTGCCA450.087.999893
GCTGCAA600.087.999893
TGCTGCC450.087.999892
AGCTGCT1150.087.999892
AGCTGCC1750.087.999892
CAGCTGC5400.086.370271
CAGCAGC3250.082.584511
AGCAGCG750.082.133232
CAGCCCT172450.082.131531
CAGCGTA68600.081.906611
GCTGCCT650.081.2306753
AGCTGCA1300.081.2306752
GCTGCTT600.080.6665653
TGCTGCA600.080.6665652
AGCAGCA1600.079.74992
CTGCTGC2100.079.618951
AGCCCTT149600.079.029312
GCAGCAA950.078.736753
Produced by FastQC (version 0.11.5)