##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1SN_8A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3269810 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.755341747685645 12.0 12.0 12.0 2.0 27.0 2 14.690767965111123 12.0 12.0 12.0 12.0 22.0 3 13.719361369620865 12.0 12.0 12.0 12.0 22.0 4 13.775335569956663 12.0 12.0 12.0 12.0 22.0 5 22.440580645358597 22.0 22.0 22.0 22.0 27.0 6 26.869508320055292 27.0 22.0 32.0 22.0 32.0 7 34.98619980977488 37.0 37.0 37.0 27.0 41.0 8 37.62561035656506 41.0 37.0 41.0 32.0 41.0 9 38.5559601322401 41.0 41.0 41.0 32.0 41.0 10-11 38.90854422734043 41.0 41.0 41.0 37.0 41.0 12-13 39.08414540906047 41.0 41.0 41.0 37.0 41.0 14-15 39.04120147653839 41.0 41.0 41.0 37.0 41.0 16-17 38.95063321722057 41.0 41.0 41.0 37.0 41.0 18-19 38.88735094699692 41.0 41.0 41.0 37.0 41.0 20-21 38.898136588976115 41.0 41.0 41.0 37.0 41.0 22-23 38.88432569476514 41.0 41.0 41.0 37.0 41.0 24-25 38.905039130714016 41.0 41.0 41.0 37.0 41.0 26-27 38.86917710815001 41.0 41.0 41.0 37.0 41.0 28-29 38.81014294408544 41.0 41.0 41.0 37.0 41.0 30-31 38.69015447380735 41.0 41.0 41.0 32.0 41.0 32-33 38.738494744342944 41.0 41.0 41.0 32.0 41.0 34-35 38.709401310779526 41.0 41.0 41.0 32.0 41.0 36-37 38.63478948318098 41.0 41.0 41.0 32.0 41.0 38-39 38.538649799223805 41.0 41.0 41.0 32.0 41.0 40-41 38.59184998516733 41.0 41.0 41.0 32.0 41.0 42-43 38.33745095280766 41.0 41.0 41.0 32.0 41.0 44-45 38.44323202265575 41.0 41.0 41.0 32.0 41.0 46-47 38.45291087249718 41.0 41.0 41.0 32.0 41.0 48-49 38.401706674088096 41.0 41.0 41.0 32.0 41.0 50-51 38.284211620858706 41.0 41.0 41.0 32.0 41.0 52-53 38.254715717427004 41.0 41.0 41.0 32.0 41.0 54-55 38.131611469779585 41.0 39.0 41.0 32.0 41.0 56-57 37.915683938822134 41.0 37.0 41.0 29.5 41.0 58-59 37.90724889213746 41.0 37.0 41.0 29.5 41.0 60-61 37.909506362755025 41.0 37.0 41.0 29.5 41.0 62-63 37.896475330370876 41.0 37.0 41.0 29.5 41.0 64-65 37.86442897293726 41.0 37.0 41.0 29.5 41.0 66-67 37.76480514158315 41.0 37.0 41.0 27.0 41.0 68-69 37.778043678378864 41.0 37.0 41.0 27.0 41.0 70-71 37.74433330988651 41.0 37.0 41.0 27.0 41.0 72-73 37.622413840559545 41.0 37.0 41.0 27.0 41.0 74-75 37.52178261122206 41.0 37.0 41.0 27.0 41.0 76-77 36.48263996990651 39.0 34.5 41.0 24.5 41.0 78-79 37.009879473119234 41.0 37.0 41.0 27.0 41.0 80-81 37.65165728283907 41.0 37.0 41.0 27.0 41.0 82-83 37.83249806563684 41.0 37.0 41.0 32.0 41.0 84-85 37.73913943011979 41.0 37.0 41.0 27.0 41.0 86-87 37.606904682535074 41.0 37.0 41.0 27.0 41.0 88-89 37.50813059474404 41.0 37.0 41.0 27.0 41.0 90-91 37.34763763032103 41.0 37.0 41.0 27.0 41.0 92-93 37.23731807046893 41.0 37.0 41.0 27.0 41.0 94-95 37.07524183362336 41.0 37.0 41.0 27.0 41.0 96-97 37.017722589385926 41.0 37.0 41.0 27.0 41.0 98-99 36.888527926699105 41.0 37.0 41.0 27.0 41.0 100 36.77005269419324 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2920175949411572 1101 2 -0.5451342426576726 1101 3 0.027367749175420286 1101 4 0.19779551040836552 1101 5 -1.0170828025974323 1101 6 -0.6377125811157214 1101 7 -0.30434504352076885 1101 8 -0.25134242841883037 1101 9 -0.2129841912435282 1101 10-11 -0.15060358841986954 1101 12-13 -0.022341753772970208 1101 14-15 0.031103938324619662 1101 16-17 -0.2201806282745622 1101 18-19 0.01905941401800959 1101 20-21 0.07657144344628364 1101 22-23 0.002373982781200823 1101 24-25 -0.12363436156845609 1101 26-27 0.010303710364752305 1101 28-29 0.01237240509913562 1101 30-31 -0.12209244762871663 1101 32-33 -0.10253211639016513 1101 34-35 -0.022144538996194285 1101 36-37 -0.04228249008731666 1101 38-39 -0.007249260512985245 1101 40-41 0.007752461868179239 1101 42-43 0.07178251415176362 1101 44-45 -0.12893091203710583 1101 46-47 -0.01733572966899999 1101 48-49 -0.03140951476442666 1101 50-51 0.01310978222974768 1101 52-53 -0.03596983515104313 1101 54-55 -0.08350921990625437 1101 56-57 0.055237002876545205 1101 58-59 -0.2036794693075592 1101 60-61 -0.0690596508216359 1101 62-63 -0.019767183194616678 1101 64-65 0.09120825995910309 1101 66-67 -0.09301845994405511 1101 68-69 0.003367313201628974 1101 70-71 -0.2238936770142388 1101 72-73 -0.1085594901871616 1101 74-75 0.08095634136818575 1101 76-77 -0.43312493388795303 1101 78-79 -0.27020207684540054 1101 80-81 -0.09859912216514743 1101 82-83 -0.21598253365611697 1101 84-85 -0.09371301152653189 1101 86-87 -0.12359092422829576 1101 88-89 -0.40080563108512735 1101 90-91 -0.2093606807158821 1101 92-93 0.15352203409091913 1101 94-95 -0.07428547904743965 1101 96-97 -0.13758996352609643 1101 98-99 -0.46201823129652553 1101 100 -0.78831945521884 1102 1 1.8248244958175182 1102 2 0.6571978969862116 1102 3 0.023242586035888024 1102 4 0.15202398043514265 1102 5 -0.8394631165602782 1102 6 -0.2471872984887007 1102 7 0.07947684900450724 1102 8 -0.27333144457927006 1102 9 -0.3101980465759553 1102 10-11 -0.22889337638885365 1102 12-13 -0.3837997500105317 1102 14-15 -0.26704815454370134 1102 16-17 -0.27239843688833787 1102 18-19 -0.184703671224689 1102 20-21 -0.1686357808049337 1102 22-23 -0.2223896797472591 1102 24-25 -0.15011672353255534 1102 26-27 -0.3351977281080849 1102 28-29 -0.3551256360410733 1102 30-31 -0.35686164593644776 1102 32-33 -0.28283155818921557 1102 34-35 -0.2898402442035106 1102 36-37 -0.14894741422817503 1102 38-39 -0.30224667043210474 1102 40-41 -0.2639695448103083 1102 42-43 -0.23800182753502952 1102 44-45 -0.20166210194515344 1102 46-47 -0.41365574038493946 1102 48-49 -0.4305776661342122 1102 50-51 -0.5001485276624678 1102 52-53 -0.2913899201910439 1102 54-55 -0.25590435126543554 1102 56-57 -0.20681671196324913 1102 58-59 -0.22381980783173105 1102 60-61 -0.17103919570234183 1102 62-63 -0.4192086858919808 1102 64-65 -0.2751745053937995 1102 66-67 -0.3789873931982086 1102 68-69 -0.3874869156240095 1102 70-71 -0.5093397339865362 1102 72-73 -0.4095473363659963 1102 74-75 -0.47655376705959185 1102 76-77 -0.3711268139191972 1102 78-79 -0.3687099650345189 1102 80-81 -0.37102916331010505 1102 82-83 -0.33605477142672413 1102 84-85 -0.5055623648360807 1102 86-87 -0.40480098393648234 1102 88-89 -0.40089062367049877 1102 90-91 -0.5453306808225662 1102 92-93 -0.4618971856131324 1102 94-95 -0.5348178621707831 1102 96-97 -0.3902277564215666 1102 98-99 -0.6463929905138315 1102 100 -0.7089802399718579 1103 1 1.6219210742612464 1103 2 0.7136512415224576 1103 3 0.4424462456540539 1103 4 1.2155538315297605 1103 5 2.9995136840745538 1103 6 -0.07467177778665146 1103 7 -0.4591890708013153 1103 8 -0.2381132211745225 1103 9 -0.37189061997837314 1103 10-11 -0.16358039501369603 1103 12-13 -0.4133707718095323 1103 14-15 -0.4296621675204122 1103 16-17 -0.2950731323091418 1103 18-19 -0.2046728632146042 1103 20-21 -0.2599124460712261 1103 22-23 -0.21114942394742542 1103 24-25 -0.26074455343499636 1103 26-27 -0.26655748164399995 1103 28-29 -0.2335460253422923 1103 30-31 -0.09891177897103631 1103 32-33 -0.26846662330613924 1103 34-35 -0.35268204973961303 1103 36-37 -0.36625591443822714 1103 38-39 -0.1921033665064087 1103 40-41 -0.2864304513126683 1103 42-43 -0.21699449896293288 1103 44-45 -0.40785731269995296 1103 46-47 -0.5050294975618073 1103 48-49 -0.39932340669341926 1103 50-51 -0.2295589743786124 1103 52-53 -0.2510351612870636 1103 54-55 -0.2601931996993656 1103 56-57 -0.17745574477844883 1103 58-59 -0.43466236022868543 1103 60-61 -0.27487432616959495 1103 62-63 -0.16010398533649806 1103 64-65 -0.51021908365081 1103 66-67 -0.4966851946265791 1103 68-69 -0.4569683141209495 1103 70-71 -0.5401178696351394 1103 72-73 -0.4346764032567165 1103 74-75 -0.3690646198813212 1103 76-77 -0.6314302261397131 1103 78-79 -0.5946699146214129 1103 80-81 -0.33789188095001776 1103 82-83 -0.38739398628926125 1103 84-85 -0.39165021434362046 1103 86-87 -0.28229806651128087 1103 88-89 -0.22140689123339996 1103 90-91 -0.5378956810559501 1103 92-93 -0.30653052361434874 1103 94-95 -0.32014671433901754 1103 96-97 -0.22676418922743835 1103 98-99 -0.23454217596871985 1103 100 -0.1812166214310409 1104 1 1.6339577627603301 1104 2 -0.6147434484321206 1104 3 -0.06383182907805995 1104 4 0.12219515251648616 1104 5 -1.187296524462674 1104 6 -0.40508596634847294 1104 7 -0.206120737844401 1104 8 -0.15237681624591204 1104 9 0.012473354023484262 1104 10-11 -0.10021243094935528 1104 12-13 -0.015417874622656313 1104 14-15 -0.10939622609151911 1104 16-17 -0.0500656038893581 1104 18-19 -0.1286380191783678 1104 20-21 0.024750845353892714 1104 22-23 0.026976062263024403 1104 24-25 -0.15382587291604466 1104 26-27 -0.1451357395676638 1104 28-29 -0.03568848813934977 1104 30-31 -0.07196392582540767 1104 32-33 -0.0495726736461819 1104 34-35 -0.1742047353778986 1104 36-37 -0.05280858828198376 1104 38-39 -0.15085421345363415 1104 40-41 -0.12138327321535058 1104 42-43 -0.07150985693929357 1104 44-45 -0.09893870746518729 1104 46-47 -0.25601132897539003 1104 48-49 -0.1839962968537776 1104 50-51 0.00925078245342803 1104 52-53 -0.13503940229686862 1104 54-55 -0.25655063652337873 1104 56-57 -0.20762055443490368 1104 58-59 -0.1849938616465323 1104 60-61 -0.15180398563407493 1104 62-63 -0.18124629756515986 1104 64-65 -0.38319945956990864 1104 66-67 -0.6788831167827212 1104 68-69 -0.5613586375920576 1104 70-71 -0.21996071387213334 1104 72-73 -0.061446021063936485 1104 74-75 -0.13006759360714426 1104 76-77 -0.11725571736909757 1104 78-79 -0.20977424597225536 1104 80-81 -0.4578255681190839 1104 82-83 -0.3118461055394306 1104 84-85 -0.29933284126023807 1104 86-87 -0.2751693430803286 1104 88-89 -0.3077077394353651 1104 90-91 -0.23992057503071607 1104 92-93 -0.33262961016608017 1104 94-95 -0.4202232532734911 1104 96-97 -0.3704504172381746 1104 98-99 -0.37246257547685957 1104 100 -0.11028736348227852 1105 1 1.7278416794579634 1105 2 0.453791174630334 1105 3 -0.10616606883209734 1105 4 -0.1690945189924271 1105 5 -1.2143883254841263 1105 6 0.3695701891026779 1105 7 0.09931135309724937 1105 8 -0.13930405005924484 1105 9 0.02537928695026892 1105 10-11 -0.15531949709405524 1105 12-13 -0.19168393502445724 1105 14-15 -0.12852257912125253 1105 16-17 -0.22967313044585325 1105 18-19 -0.11030838498249551 1105 20-21 -0.1734308041397412 1105 22-23 -0.15171046068218885 1105 24-25 -0.025513272516029417 1105 26-27 -0.14827040449446827 1105 28-29 -0.24541145851072343 1105 30-31 -0.15504004788110848 1105 32-33 -0.1763917562984929 1105 34-35 -0.096460616310317 1105 36-37 -0.13340051508851047 1105 38-39 -0.1643226385188612 1105 40-41 -0.02076325360824427 1105 42-43 0.08117612253757756 1105 44-45 -0.1440752058775132 1105 46-47 -0.4282013767833206 1105 48-49 -0.369662991365999 1105 50-51 -0.23685804180177428 1105 52-53 -0.20822867330994654 1105 54-55 -0.05226937101028284 1105 56-57 0.012857940157779524 1105 58-59 -0.04837878606507218 1105 60-61 -0.1542752783109833 1105 62-63 -0.32246645121499284 1105 64-65 -0.15352251117347748 1105 66-67 -0.13399236736291442 1105 68-69 -0.42519269154905004 1105 70-71 -0.308315788339776 1105 72-73 -0.24658226982649722 1105 74-75 -0.41443350479519125 1105 76-77 -0.28208265726784276 1105 78-79 -0.14370929526368315 1105 80-81 -0.4370832129231488 1105 82-83 -0.36617427640357647 1105 84-85 -0.2781857053672212 1105 86-87 -0.2979834312969558 1105 88-89 -0.3669552110112946 1105 90-91 -0.37081555025297774 1105 92-93 -0.3082026404053906 1105 94-95 -0.13397698721789197 1105 96-97 -0.28241340617964994 1105 98-99 -0.2963393195445576 1105 100 -0.27891378084484586 1106 1 1.1649462630926664 1106 2 -0.6358612973772768 1106 3 -0.06410062987604626 1106 4 -0.2211749205019693 1106 5 -1.1749138074361305 1106 6 -0.6728662749198691 1106 7 -0.33510506896311654 1106 8 -0.40700996454575744 1106 9 -0.29700302440608084 1106 10-11 -0.3295790194512094 1106 12-13 -0.1785887799580479 1106 14-15 -0.1410964227273439 1106 16-17 -0.12093210322802861 1106 18-19 -0.044549679435142764 1106 20-21 -0.061690067581821495 1106 22-23 -0.18803781524868413 1106 24-25 -0.2913731484798916 1106 26-27 -0.2650059952037793 1106 28-29 -0.20236393602942115 1106 30-31 -0.12072129132488385 1106 32-33 -0.265809505387125 1106 34-35 -0.27815179199128437 1106 36-37 -0.35215177263808783 1106 38-39 -0.32689245206578477 1106 40-41 -0.3885799681694806 1106 42-43 -0.10908957932230834 1106 44-45 -0.10999547962415335 1106 46-47 -0.5116883303075923 1106 48-49 -0.3445026770284585 1106 50-51 -0.41399377549263505 1106 52-53 -0.38875838947930674 1106 54-55 -0.3503142553664631 1106 56-57 -0.41113142662256763 1106 58-59 -0.2587499397630282 1106 60-61 -0.0749856048567068 1106 62-63 -0.3337425331619741 1106 64-65 -0.621475811107473 1106 66-67 -0.5403479535854672 1106 68-69 -0.4346737155168725 1106 70-71 -0.8314079683610416 1106 72-73 -0.5909462232307519 1106 74-75 -0.7322034930920935 1106 76-77 -0.6033963243844838 1106 78-79 -0.31936039764122626 1106 80-81 -0.39979743594013684 1106 82-83 -0.4077650180536452 1106 84-85 -0.6676579989169653 1106 86-87 -0.579009546906434 1106 88-89 -0.4951726940098169 1106 90-91 -0.5821343874103775 1106 92-93 -0.41484909895105915 1106 94-95 -0.41745943274040087 1106 96-97 -0.3921674356782603 1106 98-99 -0.6712048023061143 1106 100 -0.6670886077617695 1107 1 1.2738626710304288 1107 2 -0.5899523620811458 1107 3 -0.12285353688710643 1107 4 -0.254747504288817 1107 5 -1.2491349645740968 1107 6 -0.22206848483853392 1107 7 -0.16100019226760764 1107 8 -0.16223561943485265 1107 9 -0.2378676314726249 1107 10-11 -0.1967848063215527 1107 12-13 -0.2825848134373743 1107 14-15 -0.3356220929630922 1107 16-17 -0.27235645010970444 1107 18-19 -0.24280116392911566 1107 20-21 -0.2128341608894999 1107 22-23 -0.12628611235997056 1107 24-25 -0.18193436393515583 1107 26-27 -0.23974480690029054 1107 28-29 -0.2022863173584497 1107 30-31 -0.2829580666380096 1107 32-33 -0.078095390003881 1107 34-35 -0.19573268658335508 1107 36-37 -0.18391823192813206 1107 38-39 -0.17902321739178007 1107 40-41 -0.2878082083800848 1107 42-43 -0.10557790996013239 1107 44-45 0.04171708928824813 1107 46-47 -0.20991921486531595 1107 48-49 -0.18465425478288466 1107 50-51 -0.19080780942054787 1107 52-53 -0.1294213060525209 1107 54-55 -0.30120748985158485 1107 56-57 -0.178375285008336 1107 58-59 -0.2667064733072806 1107 60-61 -0.18798398755512125 1107 62-63 -0.27623477129487384 1107 64-65 -0.3691364492923199 1107 66-67 -0.24877745930505313 1107 68-69 -0.4323100351523266 1107 70-71 -0.19713557699767392 1107 72-73 -0.13766180244900994 1107 74-75 -0.21111960590182832 1107 76-77 -0.28735753275667264 1107 78-79 -0.5854838319870552 1107 80-81 -0.46184031213983445 1107 82-83 -0.3137779564663319 1107 84-85 -0.2290624541696502 1107 86-87 -0.1296062367504902 1107 88-89 -0.09484274087613898 1107 90-91 -0.26289723433337286 1107 92-93 -0.27460756617414717 1107 94-95 -0.11176056957356195 1107 96-97 -0.09371355238369006 1107 98-99 -0.3452706630765121 1107 100 -0.34253690723408425 1108 1 1.405411643247998 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0.11543835613267106 2223 42-43 0.24052714270570874 2223 44-45 0.3504131155165382 2223 46-47 0.019645042116103184 2223 48-49 0.1454594476376485 2223 50-51 0.13334802567605664 2223 52-53 0.13931389984419695 2223 54-55 0.3944047360651197 2223 56-57 0.3923127425220727 2223 58-59 0.333370723428132 2223 60-61 0.2488237414192369 2223 62-63 0.21776562398961374 2223 64-65 0.09436263225255459 2223 66-67 0.3366717698901951 2223 68-69 0.3735663338108637 2223 70-71 0.39786430674218565 2223 72-73 0.41862066319611557 2223 74-75 0.19221857160415823 2223 76-77 0.3149551140217568 2223 78-79 0.28616264416799453 2223 80-81 0.2740473340573004 2223 82-83 0.17876628051834587 2223 84-85 0.19982075088998386 2223 86-87 0.36179365163977195 2223 88-89 0.3725253619908315 2223 90-91 0.3791498742768553 2223 92-93 0.3078892366719046 2223 94-95 0.5501785333631659 2223 96-97 0.40051901440776305 2223 98-99 0.3350019669604549 2223 100 0.64184121725728 2224 1 1.302436787791967 2224 2 -0.9812211206077492 2224 3 -0.09074881844141913 2224 4 -0.17925707760584153 2224 5 -1.0170067244649559 2224 6 0.47029214930551433 2224 7 0.24256098757903288 2224 8 0.15385696183249564 2224 9 0.09696795209984543 2224 10-11 0.15154656687315793 2224 12-13 0.0953177598326107 2224 14-15 0.09393522933073939 2224 16-17 0.015118969751988232 2224 18-19 0.05735901859899428 2224 20-21 0.00438698979027663 2224 22-23 0.09465757677772046 2224 24-25 0.03988785933247385 2224 26-27 0.13414732275111874 2224 28-29 0.021999800155789728 2224 30-31 -0.03470243319961952 2224 32-33 0.006130982485188952 2224 34-35 0.040326258705256635 2224 36-37 -0.05238320817124986 2224 38-39 -0.03622508030752414 2224 40-41 0.0808197729907647 2224 42-43 0.18390037954449667 2224 44-45 0.21856894985182151 2224 46-47 -0.0017233264520939429 2224 48-49 0.04889586988193173 2224 50-51 0.014364369155913437 2224 52-53 0.03383056391972161 2224 54-55 0.2478796343898395 2224 56-57 0.21574571042846458 2224 58-59 0.2158296324461162 2224 60-61 0.12604974251026846 2224 62-63 0.25931116651384656 2224 64-65 0.14275751504884227 2224 66-67 0.20116444218622576 2224 68-69 0.2556903882376176 2224 70-71 0.25167704943127234 2224 72-73 0.15896001624600586 2224 74-75 0.3144479718372608 2224 76-77 0.2901244731707848 2224 78-79 0.30804065406460523 2224 80-81 0.1772289356373733 2224 82-83 0.04312682784001254 2224 84-85 0.0068824110512153425 2224 86-87 0.26763396647668714 2224 88-89 0.09948030564970622 2224 90-91 0.1328145312026905 2224 92-93 0.12027717767493584 2224 94-95 0.2627638223914346 2224 96-97 0.20424523766193659 2224 98-99 0.15367015641823656 2224 100 0.43551973293151036 2225 1 1.298151862966332 2225 2 1.5047015104342751 2225 3 0.8954419418300112 2225 4 0.9387506404016897 2225 5 3.216046666680189 2225 6 -0.02015338001945466 2225 7 0.30299853146853906 2225 8 0.3975452886032471 2225 9 0.17139473528779092 2225 10-11 0.24743572734581676 2225 12-13 0.17411911698261662 2225 14-15 0.20252815165414262 2225 16-17 0.21473662261369952 2225 18-19 0.21744829838564783 2225 20-21 0.1444531875541628 2225 22-23 0.20335452482657246 2225 24-25 0.1792707600278831 2225 26-27 0.1550839610323962 2225 28-29 0.213612030588898 2225 30-31 0.17647411411876845 2225 32-33 0.22816243892950183 2225 34-35 0.22605928240816553 2225 36-37 0.12226207579326598 2225 38-39 0.1776028372984868 2225 40-41 0.16937254839711358 2225 42-43 0.43140703485326526 2225 44-45 0.3322578470108155 2225 46-47 0.30507121315576313 2225 48-49 0.25990153449176745 2225 50-51 0.14515825141469207 2225 52-53 0.2022568627451946 2225 54-55 0.28722660530097244 2225 56-57 0.4140909767202956 2225 58-59 0.48985115945961155 2225 60-61 0.21767104305157403 2225 62-63 0.15723820263625754 2225 64-65 0.19166842414485075 2225 66-67 0.39390517641616185 2225 68-69 0.337447829232822 2225 70-71 0.20635713175418857 2225 72-73 0.3150081888062175 2225 74-75 0.34897457764382267 2225 76-77 0.5248232240205226 2225 78-79 0.513927668568364 2225 80-81 0.38386435095014093 2225 82-83 0.316780290797972 2225 84-85 0.366339214427164 2225 86-87 0.3173367567972889 2225 88-89 0.4880328706669488 2225 90-91 0.5315583445940959 2225 92-93 0.40641793546845406 2225 94-95 0.6529423445622484 2225 96-97 0.5925342318804496 2225 98-99 0.6158660389631407 2225 100 0.6870286903646488 2226 1 1.4500123874589423 2226 2 1.475869995230573 2226 3 0.8356169239311164 2226 4 0.8756942150744003 2226 5 3.248940010096625 2226 6 -0.2602409335685749 2226 7 0.1547685950712463 2226 8 0.09949806792612748 2226 9 0.3174981000486454 2226 10-11 0.17057169020222318 2226 12-13 0.18918497542706092 2226 14-15 0.15088147642748595 2226 16-17 0.32213288830113385 2226 18-19 0.16062830421422092 2226 20-21 0.16857665092850027 2226 22-23 0.21038457205465733 2226 24-25 0.3143279658928364 2226 26-27 0.2710291627511481 2226 28-29 0.29502266492853124 2226 30-31 0.2080924288842354 2226 32-33 0.2962297447944806 2226 34-35 0.21739964289526625 2226 36-37 0.2138074315825662 2226 38-39 0.22875426924483833 2226 40-41 0.3057555340648008 2226 42-43 0.43180147025856996 2226 44-45 0.24249711467574997 2226 46-47 0.3595067712297748 2226 48-49 0.2845010864927744 2226 50-51 0.28889662407698324 2226 52-53 0.2860361349331697 2226 54-55 0.4604738503293859 2226 56-57 0.5248745490412077 2226 58-59 0.45652414542590236 2226 60-61 0.47399740270213186 2226 62-63 0.5190356406116337 2226 64-65 0.46293315673547397 2226 66-67 0.47968746983119104 2226 68-69 0.3915644131351783 2226 70-71 0.47001694603159194 2226 72-73 0.39909104200008727 2226 74-75 0.44841066695565956 2226 76-77 0.5983051627446159 2226 78-79 0.32108955305294984 2226 80-81 0.4804227863008208 2226 82-83 0.3906782500808461 2226 84-85 0.43037704087512907 2226 86-87 0.5724549844119764 2226 88-89 0.3861451592973779 2226 90-91 0.29845598976380217 2226 92-93 0.3554100187517335 2226 94-95 0.5925827324329873 2226 96-97 0.4356634055405877 2226 98-99 0.4998625631168707 2226 100 0.5456130653986477 2227 1 1.3834742714802033 2227 2 1.8905503553101592 2227 3 0.6244478124382304 2227 4 -0.06348063772209933 2227 5 3.2261367745563376 2227 6 -0.010748207477213612 2227 7 0.27690975900675596 2227 8 0.36088844353366767 2227 9 0.20401064813756875 2227 10-11 0.18697807786135456 2227 12-13 0.18614878851165173 2227 14-15 0.2195784317745364 2227 16-17 0.10732074254396906 2227 18-19 0.18634566890447246 2227 20-21 0.22028474840585943 2227 22-23 0.19053945922923532 2227 24-25 0.2605635277440044 2227 26-27 0.10202102893178733 2227 28-29 0.10217512379598048 2227 30-31 0.23333527106813534 2227 32-33 0.21540278278472869 2227 34-35 0.21730540374648655 2227 36-37 0.20272696110036037 2227 38-39 0.14296750599652341 2227 40-41 0.2341640086364123 2227 42-43 0.3083255289454101 2227 44-45 0.19957316125226043 2227 46-47 0.28773422404023563 2227 48-49 0.2245661350222079 2227 50-51 0.3429775635179766 2227 52-53 0.2505761171108958 2227 54-55 0.36131195355171286 2227 56-57 0.3645166284099517 2227 58-59 0.4378517163478932 2227 60-61 0.2701391383819143 2227 62-63 0.42393623695100047 2227 64-65 0.313718685640211 2227 66-67 0.31036937352853755 2227 68-69 0.3353483231685104 2227 70-71 0.5654368086844883 2227 72-73 0.38389249852549767 2227 74-75 0.39730454139952087 2227 76-77 0.6203551733902515 2227 78-79 0.5258847024957092 2227 80-81 0.30789310240289325 2227 82-83 0.1028063295156798 2227 84-85 0.14074451734458648 2227 86-87 0.33410347089569115 2227 88-89 0.2738650927070623 2227 90-91 0.383289750299312 2227 92-93 0.27983888247653255 2227 94-95 0.42023928987171644 2227 96-97 0.26416547685168723 2227 98-99 0.5186194231303816 2227 100 0.45233753054702674 2228 1 1.2339990863392583 2228 2 1.3965860994573145 2228 3 -0.13455541259760295 2228 4 -0.17516243418650568 2228 5 3.2098622881731558 2228 6 -0.07951164512651943 2228 7 0.24592477931253853 2228 8 0.2540740622627595 2228 9 0.19831084522309084 2228 10-11 0.17237396594066468 2228 12-13 0.2230612646225012 2228 14-15 0.22208679297854417 2228 16-17 0.26761323537722603 2228 18-19 0.08355138438464138 2228 20-21 0.16412657234278782 2228 22-23 0.23340742685942928 2228 24-25 0.26477959021413966 2228 26-27 0.15720124273972402 2228 28-29 0.19270941966315291 2228 30-31 0.3350302812210231 2228 32-33 0.1333080772185582 2228 34-35 0.05902669196913024 2228 36-37 0.21275853957622815 2228 38-39 0.1172530709750248 2228 40-41 0.22742838692116862 2228 42-43 0.4209610252425904 2228 44-45 0.3796973848547438 2228 46-47 0.2305551598978468 2228 48-49 0.26880586268585205 2228 50-51 0.21025651418687374 2228 52-53 0.1887647966378978 2228 54-55 0.11947719184602335 2228 56-57 0.4073771306794285 2228 58-59 0.4515021284358056 2228 60-61 0.3226504857302146 2228 62-63 0.23461095625344086 2228 64-65 0.24537330819157432 2228 66-67 0.30029486922848747 2228 68-69 0.4184004612478205 2228 70-71 0.44815785801559116 2228 72-73 0.36878074207542255 2228 74-75 0.3121415725748662 2228 76-77 0.45358648012699376 2228 78-79 0.3358950046935121 2228 80-81 0.27986440555626757 2228 82-83 0.32649653197625383 2228 84-85 0.3298047777363706 2228 86-87 0.40282958174137207 2228 88-89 0.3010091443076348 2228 90-91 0.30205729389701474 2228 92-93 0.24633620987739135 2228 94-95 0.3740231079558072 2228 96-97 0.3425778932396497 2228 98-99 0.37189895609741797 2228 100 0.3541829677185504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 31.0 14 154.0 15 598.0 16 2169.0 17 5258.0 18 8859.0 19 11284.0 20 13271.0 21 15252.0 22 17373.0 23 19860.0 24 22723.0 25 25994.0 26 29008.0 27 32922.0 28 37294.0 29 42533.0 30 47894.0 31 56360.0 32 65736.0 33 78040.0 34 95873.0 35 123908.0 36 173979.0 37 288406.0 38 709435.0 39 1343906.0 40 1677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.02486817453113 18.854939557574134 18.74918945616993 37.37100281172481 2 18.228398703575078 50.31127594233803 15.174728950604766 16.28559640348213 3 41.15963924509376 15.193482190096672 24.608463488704235 19.038415076105338 4 17.09506056926855 16.306054480229736 20.92873897871742 45.670145971784294 5 17.26439762555011 35.527018389447704 26.41566329542083 20.79292068958135 6 17.909572727467346 28.964068248613835 29.87286723081769 23.253491793101126 7 20.49339258244363 26.565182686455792 35.38156651303898 17.5598582180616 8 21.677589829378466 28.153287194057146 32.7509855312694 17.418137445294988 9 21.38855162838208 28.19781577522853 26.936702744196143 23.476929852193244 10-11 19.85320247965478 29.893617671974827 28.703227404650423 21.54995244371997 12-13 24.13814564148987 26.96840183374569 27.211642266676044 21.68181025808839 14-15 22.01881760713925 27.35134457353791 28.47786262810377 22.151975191219062 16-17 20.66354008336876 29.441389560861335 28.35843672873959 21.536633627030316 18-19 21.384912273190185 29.652625076074756 29.67354372272395 19.28891892801111 20-21 22.634648496395815 29.060098293173 29.669659704998146 18.635593505433036 22-23 21.43418119095605 30.083705169413516 29.007771093733275 19.474342545897162 24-25 22.047779534590696 28.530786192469897 26.635798410305185 22.78563586263422 26-27 20.309926264828842 26.690358155366827 29.948422079570374 23.051293500233957 28-29 19.777536886424723 27.91012785030769 30.735086131401594 21.577249131865997 30-31 20.221887172639114 30.07045879652222 28.0911038524398 21.616550178398867 32-33 21.110691446903644 29.854808077533555 28.67833299182521 20.356167483737586 34-35 21.4856979457522 28.982203858939815 30.03862609754084 19.493472097767146 36-37 22.09007557014016 28.033463718075364 28.763766090384458 21.112694621400017 38-39 21.39672898732561 29.668991744863764 28.830709687876794 20.103569579933833 40-41 20.742866842112505 29.488097768494065 30.04946838687184 19.719567002521586 42-43 20.80695307305693 29.68662361008574 29.187618470331266 20.318804846526064 44-45 20.837648059061536 30.181876011144375 28.488077288894466 20.492398640899623 46-47 19.94218490828537 28.050534378249974 28.98567146659767 23.021609246866987 48-49 21.14879763655992 28.415137270972927 29.951388612794016 20.48467647967313 50-51 22.229548505876487 30.576715466647908 28.05866395906796 19.135072068407645 52-53 20.279362370566105 30.090839792125895 28.51089565236672 21.118902184941284 54-55 22.20187743379757 28.687584242794827 27.87876164678385 21.231776676623753 56-57 21.167832085484132 28.78163151293706 29.206262690494096 20.844273711084714 58-59 20.92509783668107 28.058377938254726 29.242313039757274 21.774211185306932 60-61 19.89944963062006 30.31115932331112 29.50939872848648 20.279992317582337 62-63 20.35886760446721 31.145847447699488 27.272901059300064 21.222383888533237 64-65 19.844305795418506 30.049587024555276 28.946907263945548 21.159199916080674 66-67 20.32301571039296 30.177150965958266 29.431740682180312 20.068092641468464 68-69 21.147620198115487 29.860894058064535 29.156021297873576 19.835464445946403 70-71 19.54044730427762 30.663463626326916 29.59694905820216 20.19914001119331 72-73 20.11627586924011 30.774035800245276 28.453350500487794 20.65633783002682 74-75 20.328887611206767 29.019392564093938 30.128249041993264 20.52347078270603 76-77 19.690547768830605 30.376795593627765 28.741715879515933 21.190940758025693 78-79 19.756621944394322 29.54975977197452 28.2995189322927 22.394099351338458 80-81 20.85125363898292 29.52982459788837 28.276200906416015 21.34272085671269 82-83 20.587021875888816 28.059397946669684 29.68262681929531 21.670953358146193 84-85 20.56258620531468 29.167535728375654 28.587777271462254 21.682100794847408 86-87 22.134925270887297 28.05716540104777 29.29095268532422 20.51695664274071 88-89 22.35160452748019 28.031429960762246 29.061550976968082 20.555414534789485 90-91 22.581541435129257 28.73624155531973 28.715231160220316 19.966985849330694 92-93 22.863683210951095 29.4260675696753 28.697523709328678 19.012725510044927 94-95 22.64682045745777 29.56214581275365 28.350347573712238 19.440686156076346 96-97 22.06238282958337 29.600297876635036 28.62404849211422 19.713270801667377 98-99 23.229346659286012 29.834179967643383 28.158746226844983 18.777727146225622 100 22.35068704297803 30.4373342793618 27.161884023842365 20.05009465381781 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 9.0 2 19.0 3 29.5 4 33.0 5 37.0 6 43.0 7 52.5 8 70.0 9 88.0 10 96.0 11 103.5 12 126.5 13 149.0 14 170.5 15 220.0 16 289.0 17 382.5 18 534.5 19 857.0 20 1378.0 21 2203.5 22 3491.5 23 5348.5 24 8154.0 25 12297.5 26 17572.5 27 23396.5 28 29989.0 29 38053.0 30 47376.5 31 57828.5 32 69377.0 33 81892.5 34 95853.0 35 111309.5 36 126389.5 37 137633.0 38 145049.5 39 150627.5 40 155129.5 41 156688.0 42 154999.5 43 152456.0 44 150681.0 45 148038.0 46 142222.5 47 134662.5 48 123717.5 49 114250.5 50 108367.5 51 100071.0 52 89878.0 53 78769.0 54 66152.0 55 52276.0 56 39426.5 57 29212.0 58 20975.5 59 14920.0 60 11400.5 61 9380.0 62 7716.5 63 5852.5 64 4196.5 65 3668.5 66 4407.5 67 4382.0 68 2950.5 69 2654.5 70 3741.5 71 3654.0 72 2027.0 73 753.0 74 308.5 75 165.0 76 122.5 77 101.5 78 75.5 79 71.0 80 63.0 81 49.5 82 40.5 83 31.0 84 21.5 85 20.0 86 25.5 87 24.5 88 18.5 89 20.0 90 21.0 91 17.5 92 12.5 93 13.0 94 14.0 95 15.5 96 18.0 97 18.5 98 42.0 99 111.5 100 120.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.331606423614828 2 0.006208311797933214 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.9053675901657896E-4 30-31 0.009495964597331343 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.410274603111496E-4 40-41 0.0013762267532364267 42-43 1.2233126695434903E-4 44-45 0.0 46-47 0.0023090026637633382 48-49 0.0 50-51 0.0 52-53 0.0012997697113899585 54-55 0.018762558069123282 56-57 0.0117590930359868 58-59 0.014022221474642256 60-61 1.8349690043152354E-4 62-63 0.01183555007783327 64-65 9.174845021576177E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.752453506472853E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3269810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.78979677569676 #Duplication Level Percentage of deduplicated Percentage of total 1 85.17629914530134 61.147892057471374 2 9.006991520010851 12.93220181564006 3 2.6198149840238347 5.642279558789891 4 1.138858808574389 3.2703376969507008 5 0.5771366459230904 2.071626126131296 6 0.3607645782344645 1.5539529453193295 7 0.24057997737176526 1.2089831378674527 8 0.1682730909788069 0.9664232803349505 9 0.12256023845529113 0.7918717150337656 >10 0.5463256463514365 6.720237850808511 >50 0.028818033289941297 1.4095858316604322 >100 0.012664014690289504 1.655353778919066 >500 6.088778641953733E-4 0.2935520517176793 >1k 3.044389303776082E-4 0.3357021533555556 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.0582816738587256E-5 0.0 0.0 0.0 0.0 9 3.0582816738587256E-5 0.0 0.0 0.0 0.0 10-11 3.0582816738587256E-5 0.0 0.0 0.0 0.0 12-13 3.0582816738587256E-5 0.0 0.0 0.0 0.0 14-15 3.0582816738587256E-5 0.0 0.0 0.0 0.0 16-17 6.116563347717451E-5 0.0 0.0 0.0 0.0 18-19 6.116563347717451E-5 0.0 0.0 0.0 0.0 20-21 6.116563347717451E-5 0.0 0.0 0.0 0.0 22-23 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 24-25 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 26-27 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 28-29 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 30-31 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 32-33 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 34-35 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 36-37 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 38-39 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 40-41 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 42-43 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 44-45 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 46-47 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 48-49 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 50-51 6.116563347717451E-5 3.0582816738587256E-5 0.0 0.0 0.0 52-53 6.116563347717451E-5 3.0582816738587256E-5 0.0 3.0582816738587256E-5 0.0 54-55 7.645704184646815E-5 3.0582816738587256E-5 0.0 3.0582816738587256E-5 0.0 56-57 9.174845021576177E-5 3.0582816738587256E-5 2.2937112553940444E-4 3.0582816738587256E-5 0.0 58-59 1.3762267532364266E-4 3.0582816738587256E-5 4.587422510788089E-4 3.0582816738587256E-5 0.0 60-61 1.8349690043152354E-4 3.0582816738587256E-5 4.587422510788089E-4 3.0582816738587256E-5 0.0 62-63 2.4466253390869805E-4 3.0582816738587256E-5 4.587422510788089E-4 3.0582816738587256E-5 0.0 64-65 3.364109841244598E-4 3.0582816738587256E-5 4.587422510788089E-4 3.0582816738587256E-5 0.0 66-67 4.12868025970928E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 68-69 5.35199292925277E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 70-71 6.116563347717452E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 72-73 6.422391515103325E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 74-75 7.339876017260942E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 76-77 7.645704184646814E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 78-79 9.480673188962049E-4 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 80-81 0.0013150611197592522 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 82-83 0.0014985580201907756 3.0582816738587256E-5 4.893250678173961E-4 3.0582816738587256E-5 0.0 84-85 0.0015750150620372438 3.0582816738587256E-5 4.893250678173961E-4 6.116563347717451E-5 0.0 86-87 0.001773803370838061 9.174845021576177E-5 4.893250678173961E-4 6.116563347717451E-5 0.0 88 0.001773803370838061 9.174845021576177E-5 4.893250678173961E-4 6.116563347717451E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 12025 0.0 80.488205 8 CACCGGC 12220 0.0 76.822334 9 CTTCACC 13665 0.0 74.229034 6 GTGTGGC 4595 0.0 70.89288 7 CCTTCAC 15345 0.0 70.323555 5 CGTAGTG 2575 0.0 68.53924 4 TTCACCG 14930 0.0 66.902214 7 CGGGGCA 1275 0.0 62.952736 94 CGGGCCC 825 0.0 62.584595 7 GGCGCGC 555 0.0 61.738857 6 ACCTTCA 6160 0.0 60.88282 4 GGCCCGA 825 0.0 60.877743 9 GCGTAGT 1685 0.0 59.89179 3 AGGCGCG 610 0.0 59.250168 5 CAGCGTA 1185 0.0 58.98474 1 CAGCTAG 1490 0.0 58.282963 1 TGTGGCA 5645 0.0 57.78958 8 GAGCTGC 55 3.8703E-7 57.766045 1 CGCTGCA 110 0.0 57.766045 1 GCCTTCA 4530 0.0 56.782043 4 >>END_MODULE