FastQCFastQC Report
Tue 7 Mar 2017
1SN_8A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_8A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3269810
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG120250.080.4882058
CACCGGC122200.076.8223349
CTTCACC136650.074.2290346
GTGTGGC45950.070.892887
CCTTCAC153450.070.3235555
CGTAGTG25750.068.539244
TTCACCG149300.066.9022147
CGGGGCA12750.062.95273694
CGGGCCC8250.062.5845957
GGCGCGC5550.061.7388576
ACCTTCA61600.060.882824
GGCCCGA8250.060.8777439
GCGTAGT16850.059.891793
AGGCGCG6100.059.2501685
CAGCGTA11850.058.984741
CAGCTAG14900.058.2829631
TGTGGCA56450.057.789588
GAGCTGC553.8703E-757.7660451
CGCTGCA1100.057.7660451
GCCTTCA45300.056.7820434
Produced by FastQC (version 0.11.5)