FastQCFastQC Report
Tue 7 Mar 2017
1SN_9A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_9A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3177205
Sequences flagged as poor quality0
Sequence length94
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA155600.48973862246849037No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94510.2974627070019089No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT62750.19750063341836613No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA60530.19051336001296737No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA59810.18824721728689212No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG56820.17883643013277395No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG55210.17376908320363338No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG50500.1589447328705576No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44320.13949367447174482No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT39830.12536175663830318No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC38980.12268644925335317No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38460.12104979061785437No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38220.12029440970916261No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38150.12007409027746085No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA35100.11047445789616975No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC34640.10902664448784387No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG33520.10550153358061566No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG32440.10210231949150275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCT356.0754246E-1087.9998863
AGCTGCT1400.087.9998862
GCAGCTA202.5504647E-587.9998863
CAGCTGC5000.085.359891
AGCTGCC1600.085.249892
GCTGCCC1300.084.615283
AGCTGCA1250.084.47992
CAGCCCT174250.081.889141
TGCTGCC700.081.714182
CAGCAGC3300.081.333231
CAGCGTA68100.080.375821
CTGCAGC1100.079.999891
TGCAGCA550.079.999892
AGCCCTT147900.079.75922
GCTGCAT501.8189894E-1279.19993
CTGCTGC1900.078.736741
GCTGCTT455.0931703E-1178.222123
GCTGCCA455.0931703E-1178.222123
AGCAGCA1300.077.8460542
GCCCTTC134800.077.783293
Produced by FastQC (version 0.11.5)