##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1SN_9A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3177205
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.786491586158274	12.0	12.0	12.0	2.0	27.0
2	14.708008768713382	12.0	12.0	12.0	12.0	22.0
3	13.694067584559384	12.0	12.0	12.0	12.0	22.0
4	13.792858817734455	12.0	12.0	12.0	12.0	22.0
5	22.447445160132883	22.0	22.0	22.0	22.0	27.0
6	26.888260593823816	27.0	22.0	32.0	22.0	32.0
7	35.009183228655374	37.0	37.0	37.0	27.0	41.0
8	37.633129432945	41.0	37.0	41.0	32.0	41.0
9	38.57099211413806	41.0	41.0	41.0	32.0	41.0
10-11	38.92371282306304	41.0	41.0	41.0	37.0	41.0
12-13	39.10369208156226	41.0	41.0	41.0	37.0	41.0
14-15	39.06336733071993	41.0	41.0	41.0	37.0	41.0
16-17	38.971540237409926	41.0	41.0	41.0	37.0	41.0
18-19	38.908704820746536	41.0	41.0	41.0	37.0	41.0
20-21	38.92775536989272	41.0	41.0	41.0	37.0	41.0
22-23	38.90883292075897	41.0	41.0	41.0	37.0	41.0
24-25	38.932807451832666	41.0	41.0	41.0	37.0	41.0
26-27	38.898096125368056	41.0	41.0	41.0	37.0	41.0
28-29	38.85418111201513	41.0	41.0	41.0	37.0	41.0
30-31	38.72437220764792	41.0	41.0	41.0	32.0	41.0
32-33	38.76577447788229	41.0	41.0	41.0	32.0	41.0
34-35	38.7381569650054	41.0	41.0	41.0	32.0	41.0
36-37	38.661880804040024	41.0	41.0	41.0	32.0	41.0
38-39	38.576244686760845	41.0	41.0	41.0	32.0	41.0
40-41	38.61844057906241	41.0	41.0	41.0	32.0	41.0
42-43	38.361661428834466	41.0	41.0	41.0	32.0	41.0
44-45	38.46280646039522	41.0	41.0	41.0	32.0	41.0
46-47	38.481056463149216	41.0	41.0	41.0	32.0	41.0
48-49	38.43150331816801	41.0	41.0	41.0	32.0	41.0
50-51	38.32428439461728	41.0	41.0	41.0	32.0	41.0
52-53	38.29337704051203	41.0	41.0	41.0	32.0	41.0
54-55	38.16374816859472	41.0	41.0	41.0	32.0	41.0
56-57	37.947909562020705	41.0	37.0	41.0	29.5	41.0
58-59	37.95016657722747	41.0	37.0	41.0	29.5	41.0
60-61	37.94742753457835	41.0	37.0	41.0	32.0	41.0
62-63	37.942850083642696	41.0	37.0	41.0	32.0	41.0
64-65	37.91441313985091	41.0	37.0	41.0	32.0	41.0
66-67	37.799460374763356	41.0	37.0	41.0	27.0	41.0
68-69	37.815812482984256	41.0	37.0	41.0	27.0	41.0
70-71	37.790898919018446	41.0	37.0	41.0	27.0	41.0
72-73	37.65234663800416	41.0	37.0	41.0	27.0	41.0
74-75	37.5636291961016	41.0	37.0	41.0	27.0	41.0
76-77	36.53015874015054	39.0	34.5	41.0	24.5	41.0
78-79	37.04683062628946	41.0	37.0	41.0	27.0	41.0
80-81	37.68337611202299	41.0	37.0	41.0	27.0	41.0
82-83	37.867933136199895	41.0	37.0	41.0	32.0	41.0
84-85	37.77753969290619	41.0	37.0	41.0	27.0	41.0
86-87	37.64052697260642	41.0	37.0	41.0	27.0	41.0
88-89	37.56457688439997	41.0	37.0	41.0	27.0	41.0
90-91	37.403388984972636	41.0	37.0	41.0	27.0	41.0
92-93	37.29849317245818	41.0	37.0	41.0	27.0	41.0
94-95	37.13602002388892	41.0	37.0	41.0	27.0	41.0
96-97	37.08254771095979	41.0	37.0	41.0	27.0	41.0
98-99	36.955914553829544	41.0	37.0	41.0	27.0	41.0
100	36.81947371982607	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.6494217182858648
1101	2	-0.6060015508667913
1101	3	0.02432013338689032
1101	4	0.18190290457054914
1101	5	-1.0758707233018683
1101	6	-0.8773006126742047
1101	7	-0.3311889180242389
1101	8	-0.1858313302542598
1101	9	-0.22034921155580633
1101	10-11	-0.06475631468386212
1101	12-13	-0.13645116198831886
1101	14-15	-0.029737155757850076
1101	16-17	-0.09148645466274985
1101	18-19	-0.06146197661566788
1101	20-21	0.057222536759915954
1101	22-23	0.04322752690617904
1101	24-25	-0.07036175261038835
1101	26-27	0.12614316406294535
1101	28-29	0.03330499440956913
1101	30-31	0.10419177935253998
1101	32-33	-0.11599026689742686
1101	34-35	-0.06226879213154035
1101	36-37	0.04324918638678099
1101	38-39	0.02019501119283973
1101	40-41	0.021371518546409618
1101	42-43	0.13786015584329192
1101	44-45	0.044507589842460504
1101	46-47	-0.1226333411161562
1101	48-49	0.03043674661100937
1101	50-51	-0.004976265083413978
1101	52-53	0.07073449773948681
1101	54-55	0.08074568764684642
1101	56-57	0.13639317512412674
1101	58-59	0.0723548494759072
1101	60-61	0.0520589286890214
1101	62-63	-0.0991682892946244
1101	64-65	0.07274644790518181
1101	66-67	-0.04914848463896959
1101	68-69	-0.05940195024957973
1101	70-71	0.006076657740123892
1101	72-73	0.09494871200446653
1101	74-75	0.09938461098811047
1101	76-77	-0.2340699509824944
1101	78-79	-0.0696533093224545
1101	80-81	0.0363919621740294
1101	82-83	0.06956711167537577
1101	84-85	-0.06965940250653091
1101	86-87	-0.012658319302346399
1101	88-89	-0.1435635646490354
1101	90-91	-0.1840242539255712
1101	92-93	0.10948308724848488
1101	94-95	0.1359712617142037
1101	96-97	0.009753072630495296
1101	98-99	-0.1260716692393089
1101	100	-0.3169030522653671
1102	1	1.647298544529205
1102	2	0.8358122635366225
1102	3	-0.0035855231208845595
1102	4	0.03771163020589263
1102	5	-0.819834232109045
1102	6	-0.1728045449213731
1102	7	-0.17959050682788558
1102	8	-0.40205679152136753
1102	9	-0.3608264538346688
1102	10-11	-0.4574414864459584
1102	12-13	-0.2439950494738099
1102	14-15	-0.16303746194386548
1102	16-17	-0.136132651347026
1102	18-19	-0.04908935277243387
1102	20-21	-0.1496750383701908
1102	22-23	-0.06681717769681228
1102	24-25	-0.1297065518430074
1102	26-27	-0.30792315886264987
1102	28-29	-0.29123333895990555
1102	30-31	-0.30866563583180096
1102	32-33	-0.39393197550438686
1102	34-35	-0.31868920488717123
1102	36-37	-0.28309555691275534
1102	38-39	-0.2751371266186382
1102	40-41	-0.4146724379993003
1102	42-43	-0.28822790412000643
1102	44-45	-0.12654830099444325
1102	46-47	-0.4984758092333479
1102	48-49	-0.46360809344893994
1102	50-51	-0.2956094540942047
1102	52-53	-0.24260694779178493
1102	54-55	-0.15027159795540967
1102	56-57	-0.2270112236505213
1102	58-59	-0.21297725409082346
1102	60-61	-0.137235709612348
1102	62-63	-0.2861875550037212
1102	64-65	-0.5685051059498036
1102	66-67	-0.35265141693978563
1102	68-69	-0.3177322681434802
1102	70-71	-0.509193831933004
1102	72-73	-0.16838894758076606
1102	74-75	-0.3211801532311682
1102	76-77	-0.17631848331009792
1102	78-79	-0.35522056009019565
1102	80-81	-0.499945700074683
1102	82-83	-0.3904156289766618
1102	84-85	-0.42743016138134493
1102	86-87	-0.4387275446597343
1102	88-89	-0.5463144064037166
1102	90-91	-0.5474410188556149
1102	92-93	-0.6277165150559583
1102	94-95	-0.3221481846929706
1102	96-97	-0.272772103910647
1102	98-99	-0.3447682688509701
1102	100	-0.5871632856394484
1103	1	1.4530952817405183
1103	2	0.6957010012094855
1103	3	0.4834124014786969
1103	4	1.0933740117160191
1103	5	2.9086866380733163
1103	6	-0.10561325875795546
1103	7	-0.4540736720585272
1103	8	-0.07681917951218509
1103	9	-0.34141205501320115
1103	10-11	-0.13543690728752722
1103	12-13	-0.38959434703059515
1103	14-15	-0.4204382876781949
1103	16-17	-0.3439909977602795
1103	18-19	-0.34901474584189174
1103	20-21	-0.26934091945116734
1103	22-23	-0.22392977093571886
1103	24-25	-0.24262125652126798
1103	26-27	-0.22264799048334538
1103	28-29	-0.13922468171079316
1103	30-31	-0.16373554821608138
1103	32-33	-0.3973995380864821
1103	34-35	-0.3884836327610728
1103	36-37	-0.1472085958964584
1103	38-39	-0.18702843601769104
1103	40-41	-0.17948614196468782
1103	42-43	-0.035054145843297135
1103	44-45	-0.39137050098212
1103	46-47	-0.6401378919996219
1103	48-49	-0.26875834263925213
1103	50-51	-0.28305509930979156
1103	52-53	-0.30039491552744835
1103	54-55	-0.11055862124953109
1103	56-57	-0.14812229787966658
1103	58-59	-0.3696610945774168
1103	60-61	-0.24177748554181022
1103	62-63	-0.17279410934902728
1103	64-65	-0.3742690445634693
1103	66-67	-0.3820804526294026
1103	68-69	-0.27201813597856983
1103	70-71	-0.45607353932209094
1103	72-73	-0.2811728530322881
1103	74-75	-0.30995275857788585
1103	76-77	-0.33286460920297145
1103	78-79	-0.4349946528335664
1103	80-81	-0.41200475651830715
1103	82-83	-0.42994821339551237
1103	84-85	-0.40823053409986443
1103	86-87	-0.4244194528110228
1103	88-89	-0.3091900293794936
1103	90-91	-0.8332005790540791
1103	92-93	-0.417384838249383
1103	94-95	-0.49956370261838856
1103	96-97	-0.27236666582411573
1103	98-99	-0.43151166842956457
1103	100	-0.33931060587547535
1104	1	1.5730624493943601
1104	2	-0.514793646215411
1104	3	-0.008449574612889421
1104	4	0.10912393378118246
1104	5	-1.1275165865920904
1104	6	-0.5551713131265572
1104	7	-0.34582821878216663
1104	8	-0.18205508852628327
1104	9	0.013637545131224726
1104	10-11	0.05864117344771813
1104	12-13	-0.04385074372258657
1104	14-15	-0.09671311267906191
1104	16-17	-0.04614622401272328
1104	18-19	-0.023152660589055074
1104	20-21	-0.006201420816204006
1104	22-23	-0.0911501947962634
1104	24-25	-0.17538330388255474
1104	26-27	-0.058074543328409334
1104	28-29	-0.030555572561077327
1104	30-31	-0.15948416349508676
1104	32-33	-0.1548728854218453
1104	34-35	-0.15736183819205962
1104	36-37	-0.2577669032252743
1104	38-39	-0.16002139091835232
1104	40-41	-0.3071700393153307
1104	42-43	-0.29566574800511347
1104	44-45	-0.09587938555353759
1104	46-47	-0.3017890451728036
1104	48-49	-0.3888039699438366
1104	50-51	-0.2728107355467131
1104	52-53	-0.17310685892314126
1104	54-55	-0.30470991349834264
1104	56-57	-0.17459468900444364
1104	58-59	-0.23551674036594505
1104	60-61	-0.30316494606270084
1104	62-63	-0.31068760155396546
1104	64-65	-0.22782292561339546
1104	66-67	-0.6714317723784617
1104	68-69	-0.5131994286787247
1104	70-71	-0.36930477539484485
1104	72-73	-0.2412530288238912
1104	74-75	-0.39491432646936175
1104	76-77	-0.4810103373767589
1104	78-79	-0.3004061750137694
1104	80-81	-0.3548480817799984
1104	82-83	-0.49166394489661513
1104	84-85	-0.40165947274809355
1104	86-87	-0.38181743689656855
1104	88-89	-0.4161006189657215
1104	90-91	-0.4647146981175183
1104	92-93	-0.5063167531162023
1104	94-95	-0.2730168404271254
1104	96-97	-0.37591634836430643
1104	98-99	-0.4495785118534954
1104	100	-0.37932581497700113
1105	1	1.6651384292725524
1105	2	0.3911719740865287
1105	3	-0.10083173274996682
1105	4	-0.14984772311355776
1105	5	-1.156144570752467
1105	6	0.5413239030124757
1105	7	-0.054925402423954495
1105	8	-0.0885003247095284
1105	9	-0.14569508515427998
1105	10-11	-0.22847644287023172
1105	12-13	-0.2552137098710574
1105	14-15	-0.1658296533794683
1105	16-17	-0.18927771381907377
1105	18-19	-0.2106467176880642
1105	20-21	-0.17340834736715038
1105	22-23	-0.22198764494879697
1105	24-25	-0.05684750029711694
1105	26-27	-0.19181346037274238
1105	28-29	-0.2248318267445697
1105	30-31	-0.23536071444961237
1105	32-33	-0.2364165867582031
1105	34-35	-0.14944456286667673
1105	36-37	-0.341987287166468
1105	38-39	-0.3488898270713676
1105	40-41	-0.18371256284794413
1105	42-43	-0.12180701954123663
1105	44-45	-0.13053191823727417
1105	46-47	-0.373268736869683
1105	48-49	-0.4131014017738508
1105	50-51	-0.2846511496062192
1105	52-53	-0.33362052588385893
1105	54-55	-0.35028736823024076
1105	56-57	-0.24219498171846254
1105	58-59	-0.26918981957597765
1105	60-61	-0.3107059961362211
1105	62-63	-0.5126907046674489
1105	64-65	-0.3728580454641204
1105	66-67	-0.2782961621607498
1105	68-69	-0.44623843363361715
1105	70-71	-0.5038693594830619
1105	72-73	-0.48787549594364066
1105	74-75	-0.5986435649843855
1105	76-77	-0.48749878082733744
1105	78-79	-0.38810260136198593
1105	80-81	-0.5089443665382092
1105	82-83	-0.2993894499386016
1105	84-85	-0.39863515420707785
1105	86-87	-0.3866135323187052
1105	88-89	-0.4168254944088545
1105	90-91	-0.37742638266592365
1105	92-93	-0.3603538434110263
1105	94-95	-0.42795540065650783
1105	96-97	-0.3298723996764821
1105	98-99	-0.4957660410291709
1105	100	-0.48187334487280253
1106	1	1.332492895425709
1106	2	-0.5620228082693561
1106	3	-0.14838773796695293
1106	4	-0.15415106410558366
1106	5	-1.1862229662063761
1106	6	-0.5752972081860861
1106	7	-0.3153402036864037
1106	8	-0.14720027848868966
1106	9	-0.16020274961307024
1106	10-11	-0.12159130006153873
1106	12-13	-0.022794451536135796
1106	14-15	-0.009527885442032868
1106	16-17	-0.02557744383211258
1106	18-19	0.060133109040201305
1106	20-21	-0.08798839109380907
1106	22-23	-0.07875343357168418
1106	24-25	-0.11920418497431484
1106	26-27	-0.25772935214019554
1106	28-29	-0.07461322513331226
1106	30-31	0.04610123269843314
1106	32-33	0.011574796674359789
1106	34-35	-0.09040223018337912
1106	36-37	-0.3407968503548702
1106	38-39	-0.09104131506669688
1106	40-41	-0.11631911251899396
1106	42-43	0.10552528545138529
1106	44-45	-0.1451348252451936
1106	46-47	-0.21056601060082158
1106	48-49	-0.14909241412167518
1106	50-51	-0.142243324179276
1106	52-53	-0.08097341036433647
1106	54-55	-0.1276310731793444
1106	56-57	-0.1443213809379671
1106	58-59	-0.20897536351865398
1106	60-61	-0.2496213032677801
1106	62-63	-0.2551736722199678
1106	64-65	-0.3977705940470031
1106	66-67	-0.3620823570225724
1106	68-69	-0.3714691671051682
1106	70-71	-0.4698481252739626
1106	72-73	-0.32339509886269013
1106	74-75	-0.2088412854746906
1106	76-77	-0.3536653829217187
1106	78-79	-0.22886219205855696
1106	80-81	-0.25707332254172144
1106	82-83	-0.3831894590889888
1106	84-85	-0.5066540192023155
1106	86-87	-0.4288924159366232
1106	88-89	-0.4272277804328084
1106	90-91	-0.5048894924954794
1106	92-93	-0.561439000135401
1106	94-95	-0.41909404149019736
1106	96-97	-0.28667873133525035
1106	98-99	-0.4586923559941951
1106	100	-0.43414269663161065
1107	1	1.4811410190702325
1107	2	-0.5059488677590753
1107	3	-0.18328635771463908
1107	4	-0.15758093663849415
1107	5	-1.2320320466653598
1107	6	-0.27241299199861047
1107	7	-0.1516650105204036
1107	8	-0.29472821833318363
1107	9	-0.3290290361866326
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2220	94-95	0.5819320580827068
2220	96-97	0.47784361887998017
2220	98-99	0.6215623069798539
2220	100	0.6604831827318804
2221	1	1.40540938854779
2221	2	1.8020080169513477
2221	3	-0.1375104261216773
2221	4	-0.3088214034384702
2221	5	-0.8352741632475116
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2221	7	0.2626648943593466
2221	8	0.10885527058786693
2221	9	0.2159997061122354
2221	10-11	0.078966819326034
2221	12-13	0.05074217423879901
2221	14-15	0.12023015175482499
2221	16-17	0.12897830979566294
2221	18-19	0.09233483911774698
2221	20-21	0.05721557701470914
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2221	74-75	0.320764032486764
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2221	78-79	0.204397280699105
2221	80-81	0.1716253059469821
2221	82-83	0.21771371321348454
2221	84-85	0.18129930239477687
2221	86-87	0.04823505556966978
2221	88-89	0.2505461099462494
2221	90-91	0.14579404177934663
2221	92-93	0.14105615449977194
2221	94-95	0.20404764308626255
2221	96-97	0.40760031354940196
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2221	100	0.4852863370536227
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2222	2	1.8108093891018449
2222	3	0.4800455620079802
2222	4	0.721396561427607
2222	5	3.2359926188782744
2222	6	0.3106528351403597
2222	7	0.4649188519352734
2222	8	0.2847073779553
2222	9	0.22470780447195438
2222	10-11	0.3053166055835348
2222	12-13	0.13045279886308236
2222	14-15	0.2579423436650501
2222	16-17	0.273970330234782
2222	18-19	0.19161017809998526
2222	20-21	0.18342926941635795
2222	22-23	0.27036789823782925
2222	24-25	0.26195947055379776
2222	26-27	0.2678186311569206
2222	28-29	0.2763842700257655
2222	30-31	0.27075939544006644
2222	32-33	0.21734123555643947
2222	34-35	0.285543685287621
2222	36-37	0.29945666115970937
2222	38-39	0.2872758350007203
2222	40-41	0.20898704704075755
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2222	46-47	0.2196753295384113
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2222	54-55	0.32135364215901063
2222	56-57	0.3568379592170601
2222	58-59	0.3065594861415448
2222	60-61	0.31255615405222414
2222	62-63	0.17293872585432268
2222	64-65	0.4084045099265907
2222	66-67	0.42910805184484246
2222	68-69	0.47208862731233836
2222	70-71	0.4631392996943049
2222	72-73	0.4278650320246271
2222	74-75	0.4043630529926787
2222	76-77	0.5486774032746169
2222	78-79	0.536664823805431
2222	80-81	0.28735219742495843
2222	82-83	0.17469254888939645
2222	84-85	0.37399174220813336
2222	86-87	0.272961257632673
2222	88-89	0.342289351771079
2222	90-91	0.3097927440570345
2222	92-93	0.23482875729178687
2222	94-95	0.35240843374266717
2222	96-97	0.36736984856404575
2222	98-99	0.4421874841815594
2222	100	0.40610917410359804
2223	1	1.4565427742326182
2223	2	-1.1131384312173473
2223	3	0.12122202690857264
2223	4	0.004994978226900315
2223	5	-1.0382027034569035
2223	6	0.03894474006709814
2223	7	0.08498077699285034
2223	8	0.08884382096530175
2223	9	0.07413452995187697
2223	10-11	0.11052394985958358
2223	12-13	0.11987244422380172
2223	14-15	0.017692071482933613
2223	16-17	0.21190380322058644
2223	18-19	0.0322884602875817
2223	20-21	0.02219127830409917
2223	22-23	0.17546287674365857
2223	24-25	0.1560748254928157
2223	26-27	0.06523844862568495
2223	28-29	0.08662160509694417
2223	30-31	0.06052910664661226
2223	32-33	0.1565855216657681
2223	34-35	0.0779467463937138
2223	36-37	0.09254863792705237
2223	38-39	0.05924311948372463
2223	40-41	0.04820724527441911
2223	42-43	0.21416570318145745
2223	44-45	0.2224291317307916
2223	46-47	0.12073918452674093
2223	48-49	0.10514284140673169
2223	50-51	0.0962942279216179
2223	52-53	0.20001523713877134
2223	54-55	0.10287733505636254
2223	56-57	0.2816056496043444
2223	58-59	0.423065518625485
2223	60-61	0.25030047738716377
2223	62-63	0.2712605273035109
2223	64-65	0.08655302370586782
2223	66-67	0.3045121552682417
2223	68-69	0.32245934854176284
2223	70-71	0.2770622731219845
2223	72-73	0.41642159901043385
2223	74-75	0.20231635708053375
2223	76-77	0.40011431213663684
2223	78-79	0.32607086006028396
2223	80-81	0.21939816091097697
2223	82-83	0.13402211067135283
2223	84-85	0.11097462035756678
2223	86-87	0.2966882986117909
2223	88-89	0.2595904654455481
2223	90-91	0.2855458134784641
2223	92-93	0.33339518423191095
2223	94-95	0.47765342182079706
2223	96-97	0.3172195682599792
2223	98-99	0.38011370542356104
2223	100	0.5193389895998948
2224	1	1.200756108984402
2224	2	-1.0466180260754463
2224	3	-0.004884313263787021
2224	4	-0.29010125096587736
2224	5	-1.0217864068665428
2224	6	0.46204757872244784
2224	7	0.3097495964725141
2224	8	0.22807441442247267
2224	9	0.1488386834237545
2224	10-11	0.159401289160499
2224	12-13	0.09173513634760155
2224	14-15	0.13079926919207452
2224	16-17	0.1626767514183669
2224	18-19	0.1454767268811139
2224	20-21	0.059346569320645415
2224	22-23	0.14833950942158225
2224	24-25	0.10534870082826586
2224	26-27	0.1211863488439846
2224	28-29	0.07887864213841311
2224	30-31	0.09035208611905432
2224	32-33	0.21720150122738602
2224	34-35	0.14598983136427535
2224	36-37	0.1753253368874752
2224	38-39	0.1320111967164408
2224	40-41	0.04368873771781523
2224	42-43	0.2382460288429158
2224	44-45	0.4318360991164596
2224	46-47	0.19948751023163425
2224	48-49	0.12849745110305832
2224	50-51	0.2233660137172535
2224	52-53	0.16310370177462374
2224	54-55	0.2148790923418602
2224	56-57	0.38820915858774185
2224	58-59	0.3033613633236101
2224	60-61	0.30913013549607626
2224	62-63	0.2686876729724048
2224	64-65	0.3418772486135282
2224	66-67	0.38876340512596386
2224	68-69	0.4533091639499318
2224	70-71	0.5242541726263354
2224	72-73	0.4230859615070486
2224	74-75	0.2712095368388674
2224	76-77	0.34336591005985895
2224	78-79	0.20111497824737512
2224	80-81	0.20499162845468533
2224	82-83	0.27063165986613313
2224	84-85	0.0030246602942085588
2224	86-87	0.19132180686441558
2224	88-89	0.4180612244662356
2224	90-91	0.23974793278624418
2224	92-93	0.2342238436502484
2224	94-95	0.35480606300113493
2224	96-97	0.4012193764762486
2224	98-99	0.3298968690327655
2224	100	0.6653921793280446
2225	1	1.3198174155921567
2225	2	1.6438861699259952
2225	3	0.8714019223063953
2225	4	1.0015723825393632
2225	5	3.1901635015338883
2225	6	-0.10850198363919006
2225	7	0.34253671757170423
2225	8	0.38557659031275193
2225	9	0.12941495613065968
2225	10-11	0.15315767190296015
2225	12-13	0.1445321059269702
2225	14-15	0.12464427133018319
2225	16-17	0.1660013086508414
2225	18-19	0.02018201903072736
2225	20-21	0.1603448734621722
2225	22-23	0.22184857271940217
2225	24-25	0.16396982655573566
2225	26-27	0.14748430562890746
2225	28-29	0.04368084376820747
2225	30-31	0.05707828374126933
2225	32-33	0.14269918739068288
2225	34-35	0.0888036628087363
2225	36-37	0.15896224303325113
2225	38-39	0.13321299903909534
2225	40-41	0.100321548873751
2225	42-43	0.28354504797248836
2225	44-45	0.13266451516143007
2225	46-47	0.12513716717301548
2225	48-49	0.14309849395870344
2225	50-51	0.07923424380899036
2225	52-53	0.16627205674353718
2225	54-55	0.2852006985363644
2225	56-57	0.2985146029257493
2225	58-59	0.3564331981090092
2225	60-61	0.3813080943653162
2225	62-63	0.3286105245293527
2225	64-65	0.21855253904757177
2225	66-67	0.33875419862258127
2225	68-69	0.37277184508923966
2225	70-71	0.384850241431252
2225	72-73	0.38298193708629924
2225	74-75	0.33889335589232417
2225	76-77	0.5281033772159702
2225	78-79	0.3339631294569685
2225	80-81	0.2899947361785209
2225	82-83	0.29959768913810336
2225	84-85	0.2671655291052204
2225	86-87	0.2302728723947638
2225	88-89	0.23016416756110658
2225	90-91	0.420236323584966
2225	92-93	0.4411652449513923
2225	94-95	0.4996306479800552
2225	96-97	0.5021508231473817
2225	98-99	0.5052184951757113
2225	100	0.7157978760197636
2226	1	1.4252690975266695
2226	2	1.4308309048415921
2226	3	0.7826936754295737
2226	4	1.0613027076182835
2226	5	3.2511155708106187
2226	6	-0.2743539715515304
2226	7	0.23442227855196052
2226	8	0.32952415965053916
2226	9	0.24300636420697685
2226	10-11	0.16622089932622686
2226	12-13	0.252135445580258
2226	14-15	0.1318246208622469
2226	16-17	0.19505167068767548
2226	18-19	0.13215107012568694
2226	20-21	0.30144750155751865
2226	22-23	0.18513105057004253
2226	24-25	0.2775872278942657
2226	26-27	0.2432507276180118
2226	28-29	0.26667649236218693
2226	30-31	0.22753223567285374
2226	32-33	0.22218579185126686
2226	34-35	0.143240858765175
2226	36-37	0.2145244541625786
2226	38-39	0.19179919446191462
2226	40-41	0.27348245252373005
2226	42-43	0.32459358329781907
2226	44-45	0.20574758512275082
2226	46-47	0.2282204185393013
2226	48-49	0.2533809658436681
2226	50-51	0.1915161880368288
2226	52-53	0.29844724719136195
2226	54-55	0.22383602426846494
2226	56-57	0.4477234763802045
2226	58-59	0.42901643042169013
2226	60-61	0.574792746226656
2226	62-63	0.40151243301919237
2226	64-65	0.4793165193745139
2226	66-67	0.5331381443072516
2226	68-69	0.5704625963699996
2226	70-71	0.5564717595668185
2226	72-73	0.4715590610432372
2226	74-75	0.3977288918350368
2226	76-77	0.5956358203987833
2226	78-79	0.4540101194311177
2226	80-81	0.4349901444413504
2226	82-83	0.3211210233565467
2226	84-85	0.3119710952427326
2226	86-87	0.4974713721635027
2226	88-89	0.5058400015095756
2226	90-91	0.5112727632166241
2226	92-93	0.3748185084693816
2226	94-95	0.5766236037451264
2226	96-97	0.5325008482944966
2226	98-99	0.5240992216526905
2226	100	0.6031048486545174
2227	1	1.4029429503768274
2227	2	1.7900765461746815
2227	3	0.7341093355899453
2227	4	-0.00986595522879874
2227	5	3.2239897785226255
2227	6	0.12126364797299871
2227	7	0.21438555470705722
2227	8	0.24256666930799753
2227	9	0.31617582806755706
2227	10-11	0.30762342048897295
2227	12-13	0.2141144712052636
2227	14-15	0.28981549782350413
2227	16-17	0.19036174410070572
2227	18-19	0.13655856272077216
2227	20-21	0.15475565511249556
2227	22-23	0.33005725138481523
2227	24-25	0.3534684046217009
2227	26-27	0.2241240551062944
2227	28-29	0.2506291795941493
2227	30-31	0.18624006426784234
2227	32-33	0.16995375883644925
2227	34-35	0.16684755074281554
2227	36-37	0.15241249148644442
2227	38-39	0.18372290659014112
2227	40-41	0.26697885910193975
2227	42-43	0.31857286289064035
2227	44-45	0.323053239099778
2227	46-47	0.3086356385878162
2227	48-49	0.2812244152315486
2227	50-51	0.3697883504415671
2227	52-53	0.3459332876252148
2227	54-55	0.32098106464340503
2227	56-57	0.42322956789475086
2227	58-59	0.540397901464658
2227	60-61	0.3417606304371361
2227	62-63	0.3734000401324664
2227	64-65	0.3386531287111225
2227	66-67	0.34920381157179037
2227	68-69	0.4177646339047598
2227	70-71	0.6086600413037644
2227	72-73	0.5879800975982619
2227	74-75	0.5240210706660093
2227	76-77	0.703109565919064
2227	78-79	0.5040935129559827
2227	80-81	0.454925875237457
2227	82-83	0.38300575167286155
2227	84-85	0.34342869722219405
2227	86-87	0.4501681340986252
2227	88-89	0.3464144910614948
2227	90-91	0.4176648929867426
2227	92-93	0.4988269572310031
2227	94-95	0.6185334912121334
2227	96-97	0.4872682152621408
2227	98-99	0.5441215267596178
2227	100	0.6148796975238682
2228	1	1.1964876091230678
2228	2	1.6907117857861529
2228	3	-0.17682436596390438
2228	4	-0.12159795043982768
2228	5	3.2324731291450846
2228	6	0.02217549712629463
2228	7	0.15702596427671267
2228	8	0.39055400794421047
2228	9	0.09280511008689984
2228	10-11	0.1820695845241076
2228	12-13	0.24767959560306707
2228	14-15	0.11563365505616474
2228	16-17	0.17772394517240286
2228	18-19	0.14865005163516543
2228	20-21	0.17651243907872072
2228	22-23	0.36473226848476514
2228	24-25	0.3628326616351458
2228	26-27	0.203986831111294
2228	28-29	0.24182295333790194
2228	30-31	0.2528711327752191
2228	32-33	0.21696886798493864
2228	34-35	0.28357467999473585
2228	36-37	0.24781416012548618
2228	38-39	0.14509195356293958
2228	40-41	0.17463704472100972
2228	42-43	0.28901660282930663
2228	44-45	0.42582861241162107
2228	46-47	0.18876253845861157
2228	48-49	0.2870738063944529
2228	50-51	0.2903421913414874
2228	52-53	0.2565262239897237
2228	54-55	0.23819437032730661
2228	56-57	0.5038865414655902
2228	58-59	0.38952133791750043
2228	60-61	0.36727570158574707
2228	62-63	0.4986228576313607
2228	64-65	0.45691764935492785
2228	66-67	0.39684702918479076
2228	68-69	0.3281862006646392
2228	70-71	0.4539106974186353
2228	72-73	0.3843805462929879
2228	74-75	0.5090767528330105
2228	76-77	0.5124507022241573
2228	78-79	0.5162825924803585
2228	80-81	0.2936099540758619
2228	82-83	0.48916360812410886
2228	84-85	0.3986259955785272
2228	86-87	0.4413904077550441
2228	88-89	0.4701150924466617
2228	90-91	0.5646922410587649
2228	92-93	0.4843304951682157
2228	94-95	0.6667294344037913
2228	96-97	0.48309883843062806
2228	98-99	0.6613105766736993
2228	100	0.6049474162773549
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	26.0
13	44.0
14	140.0
15	536.0
16	1985.0
17	4973.0
18	8228.0
19	10501.0
20	12709.0
21	14557.0
22	16599.0
23	19029.0
24	21606.0
25	24681.0
26	27766.0
27	31291.0
28	35787.0
29	40755.0
30	46502.0
31	54040.0
32	63709.0
33	75559.0
34	92803.0
35	119150.0
36	168676.0
37	277410.0
38	683844.0
39	1322589.0
40	1709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.207138263026447	18.38348357344775	18.58367752020752	35.82570064331828
2	17.96384266434666	49.75980514975745	16.236208704804906	16.04014348109099
3	41.996534690081376	15.969193048607188	22.823141723621863	19.211130537689574
4	16.854373576775814	16.75554457455531	21.6497519045828	44.74032994408608
5	17.976806658682708	35.17783712413898	26.271770313845032	20.573585903333274
6	17.7968371571869	28.427218262592437	30.497559962293902	23.27838461792676
7	20.437932081814047	26.81473181617176	35.3242865978116	17.42304950420259
8	21.043873467402953	27.85190127801008	33.55571957113249	17.548505683454483
9	21.08044649306545	28.070615525280868	27.576344617360228	23.27259336429346
10-11	19.911101109308337	30.004610970963476	28.62781910515689	21.4564688145713
12-13	24.1728028251246	26.677976397494024	27.31680832681555	21.832412450565826
14-15	21.90315072524436	27.2547884067915	28.52025601117964	22.3218048567845
16-17	20.81609464922786	29.322816752460103	28.399961601470476	21.461126996841564
18-19	21.387792100289403	29.197266150594626	29.78150607216091	19.63343567695506
20-21	22.906170675168898	28.555003532979455	29.810273495100255	18.728552296751392
22-23	21.797586243254685	30.033645924641313	28.715049863008524	19.45371796909548
24-25	22.189628934865706	28.441680659573432	26.66297893903604	22.70571146652482
26-27	20.404537950808965	26.566998352325395	29.775525973300432	23.25293772356521
28-29	19.98574212925405	27.928414787583694	30.364464853797813	21.721378229364447
30-31	20.461296619055158	29.74653222503874	28.126663378338524	21.66550777756758
32-33	21.05638446370316	29.67589437886444	28.546741554290644	20.720979603141757
34-35	21.485094603590262	29.0325144269885	29.77834291460576	19.704048054815473
36-37	21.958372217090176	27.912142905478245	28.819135057385342	21.310349820046234
38-39	21.550052869285942	29.089737095102237	28.967411463420888	20.39279857219093
40-41	21.16876484719439	29.43174834738284	29.740310324558983	19.659176480863792
42-43	20.64911201742917	29.554400381215924	29.163679237266	20.632808364088906
44-45	20.781299916121245	30.06409721752295	28.62268880981869	20.53191405653711
46-47	20.196991850995385	28.003360888019763	28.8510739151434	22.948573345841453
48-49	21.12506450245806	28.06508048191421	30.04397419177771	20.765880823850022
50-51	22.02679713773584	30.263328932190397	28.196748399930126	19.513125530143633
52-53	20.6115941426861	29.942832520656836	28.256780592454383	21.18879274420268
54-55	22.18202022761776	28.31137718895768	28.12155143986608	21.385051143558478
56-57	21.54291401960593	28.44556493118534	29.25576855156488	20.75575249764385
58-59	21.292916421657818	27.83205571973422	29.13426463977718	21.740763218830782
60-61	19.853720049647556	30.070135753371176	29.38240659096993	20.69373760601134
62-63	20.71653031975656	30.82318404267408	27.348438990777897	21.111846646791466
64-65	19.957859147765863	30.150302058226075	28.61509907144714	21.27673972256092
66-67	20.36560435980681	29.981335796714408	29.321683681097067	20.331376162381716
68-69	21.026358072582664	29.6999249340222	29.153328161072388	20.12038883232275
70-71	19.871632456829193	30.54346508959919	29.12456073813304	20.460341715438567
72-73	20.201985707563725	30.471578006455356	28.35131821837118	20.97511806760974
74-75	20.246238439131247	29.042460275619607	30.17457167541912	20.536729609830022
76-77	19.8206442454925	30.37553447133565	28.541438150827535	21.262383132344308
78-79	19.943204797927738	29.24479219943315	28.30738022884894	22.50462277379017
80-81	20.767157340259352	29.294485044521952	28.448235976554177	21.490121638664522
82-83	20.781661869473325	28.127505149966716	29.392925543048054	21.6979074375119
84-85	20.887792887144517	28.803681222961693	28.35607082325503	21.95245506663876
86-87	21.602635020403152	28.49822406800946	29.389306009527242	20.509834902060145
88-89	20.897030566173726	28.14646835819533	29.829645867987743	21.126855207643196
90-91	20.349993154360515	28.06949819102009	30.062570718603297	21.51793793601609
92-93	20.29900494302382	27.910883937297086	30.589669221847505	21.200441897831585
94-95	20.349772834928814	28.28479748709951	30.075884307118994	21.289545370852682
96-97	20.230107909310227	28.58312573472596	29.88450540648148	21.302260949482328
98-99	21.377767566146975	29.00804323296734	29.289123616511997	20.325065584373686
100	20.765200860504752	29.911321428740038	28.369400148872987	20.95407756188222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	14.5
2	23.0
3	27.5
4	32.5
5	32.0
6	40.0
7	57.5
8	61.5
9	68.0
10	91.5
11	96.0
12	108.5
13	130.0
14	143.0
15	178.5
16	239.0
17	341.5
18	504.0
19	792.5
20	1266.0
21	1992.5
22	3165.0
23	4952.0
24	7469.0
25	11100.0
26	15986.5
27	21700.5
28	28159.0
29	35847.0
30	44510.0
31	54451.5
32	65519.0
33	77813.0
34	91676.5
35	107138.0
36	122243.0
37	133721.5
38	142585.5
39	149918.0
40	153386.0
41	153593.5
42	152932.0
43	151125.0
44	148785.0
45	145502.0
46	137270.5
47	125558.0
48	116453.5
49	110482.5
50	105449.0
51	98461.0
52	87868.5
53	74661.0
54	61710.0
55	50025.0
56	38871.5
57	29127.0
58	21194.5
59	15555.5
60	12450.5
61	10702.5
62	8908.0
63	6921.0
64	5292.0
65	4539.5
66	4726.5
67	4361.5
68	3196.0
69	2777.0
70	3137.5
71	2912.5
72	1816.5
73	933.5
74	547.0
75	357.0
76	244.0
77	168.0
78	130.5
79	102.5
80	71.5
81	52.0
82	47.0
83	43.5
84	35.0
85	26.5
86	26.5
87	21.0
88	14.5
89	11.5
90	16.5
91	20.5
92	19.0
93	12.5
94	11.0
95	18.5
96	20.0
97	19.5
98	43.5
99	102.0
100	105.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.328730755491069
2	0.006326315110293482
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.9342755661029113E-4
30-31	0.009819951812992866
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.284890336002871E-4
40-41	0.0014950247151191063
42-43	9.442261358646987E-5
44-45	0.0
46-47	0.0021559830102243953
48-49	1.5737102264411646E-5
50-51	0.0
52-53	0.0015422360219123413
54-55	0.019120579251260147
56-57	0.012935898061346372
58-59	0.014131917833441657
60-61	2.9900494302382126E-4
62-63	0.012023146130010496
64-65	9.442261358646987E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.5179363623058633E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3177205.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.29790949330645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60110040467247	62.743817099891196
2	8.870164655124118	13.003290521640269
3	2.479678719746901	5.452657991174592
4	1.0894887397920097	3.194289881730049
5	0.5591884700056798	2.0493672932088414
6	0.33475638248014505	1.4722165815201809
7	0.2338982093133472	1.2000974843827321
8	0.16027282614711705	0.9398130484134259
9	0.11680034420050074	0.770509895219581
>10	0.5197295284446752	6.387048699333213
>50	0.024457637882659162	1.2222634771607346
>100	0.00993951251918277	1.2930438466938863
>500	4.808555320080399E-4	0.23342269339865235
>1k	4.371413915466111E-5	0.038161486232653585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.147420452882329E-5	0.0
3	0.0	0.0	0.0	3.147420452882329E-5	0.0
4	0.0	0.0	0.0	3.147420452882329E-5	0.0
5	0.0	0.0	0.0	3.147420452882329E-5	0.0
6	0.0	0.0	0.0	3.147420452882329E-5	0.0
7	0.0	0.0	0.0	3.147420452882329E-5	0.0
8	0.0	0.0	0.0	3.147420452882329E-5	0.0
9	0.0	0.0	0.0	3.147420452882329E-5	0.0
10-11	0.0	0.0	0.0	3.147420452882329E-5	0.0
12-13	0.0	0.0	0.0	3.147420452882329E-5	0.0
14-15	0.0	0.0	0.0	3.147420452882329E-5	0.0
16-17	0.0	0.0	0.0	3.147420452882329E-5	0.0
18-19	0.0	0.0	0.0	3.147420452882329E-5	0.0
20-21	0.0	0.0	0.0	3.147420452882329E-5	0.0
22-23	0.0	1.5737102264411646E-5	0.0	3.147420452882329E-5	0.0
24-25	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
26-27	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
28-29	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
30-31	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
32-33	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
34-35	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
36-37	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
38-39	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
40-41	0.0	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
42-43	1.5737102264411646E-5	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
44-45	3.147420452882329E-5	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
46-47	4.721130679323493E-5	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
48-49	6.294840905764658E-5	3.147420452882329E-5	0.0	3.147420452882329E-5	0.0
50-51	7.868551132205823E-5	3.147420452882329E-5	0.0	6.294840905764658E-5	0.0
52-53	9.442261358646987E-5	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
54-55	1.2589681811529316E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
56-57	1.7310812490852807E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
58-59	2.2031943170176303E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
60-61	2.832678407594096E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
62-63	3.1474204528823287E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
64-65	3.6195335208146786E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
66-67	3.9342755661029113E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
68-69	4.7211306793234933E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
70-71	5.507985792544076E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
72-73	6.137469883120541E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
74-75	6.294840905764657E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
76-77	6.766953973697007E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
78-79	8.498035222782288E-4	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
80-81	0.0013219165902105781	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
82-83	0.0016681328400276344	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
84-85	0.0017468183513496926	3.147420452882329E-5	0.0	6.294840905764658E-5	3.147420452882329E-5
86-87	0.0017940296581429275	1.2589681811529316E-4	0.0	6.294840905764658E-5	3.147420452882329E-5
88	0.0017940296581429275	1.2589681811529316E-4	0.0	6.294840905764658E-5	3.147420452882329E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	10875	0.0	78.423744	8
CACCGGC	10820	0.0	76.21956	9
CTTCACC	12120	0.0	73.62095	6
CCTTCAC	13565	0.0	69.79238	5
GTGTGGC	4645	0.0	66.89508	7
CGTAGTG	2560	0.0	66.189514	4
TTCACCG	13300	0.0	65.99513	7
CGGGCCC	875	0.0	63.835293	7
CGGGGCA	1265	0.0	62.707302	94
GCGTAGT	1730	0.0	62.13142	3
GGGCCCG	915	0.0	60.5317	8
GCAGCCT	80	2.3646862E-11	59.530495	1
AGGCAGC	40	8.095316E-6	58.678608	2
GCAGCTA	40	8.100604E-6	58.672146	4
ACCTTCA	6090	0.0	58.42165	4
CAGCGTA	1265	0.0	57.72654	1
TGTGGCA	5745	0.0	56.04747	8
GCCTTCA	3985	0.0	55.948338	4
ATCGGAA	4620	0.0	55.471844	94
GGCCCGA	1000	0.0	55.386505	9
>>END_MODULE