FastQCFastQC Report
Tue 7 Mar 2017
1SN_9A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1SN_9A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3177205
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG108750.078.4237448
CACCGGC108200.076.219569
CTTCACC121200.073.620956
CCTTCAC135650.069.792385
GTGTGGC46450.066.895087
CGTAGTG25600.066.1895144
TTCACCG133000.065.995137
CGGGCCC8750.063.8352937
CGGGGCA12650.062.70730294
GCGTAGT17300.062.131423
GGGCCCG9150.060.53178
GCAGCCT802.3646862E-1159.5304951
AGGCAGC408.095316E-658.6786082
GCAGCTA408.100604E-658.6721464
ACCTTCA60900.058.421654
CAGCGTA12650.057.726541
TGTGGCA57450.056.047478
GCCTTCA39850.055.9483384
ATCGGAA46200.055.47184494
GGCCCGA10000.055.3865059
Produced by FastQC (version 0.11.5)