FastQCFastQC Report
Thu 5 Dec 2024
310_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename310_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19737649
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA8558364.336058463700514Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4990672.5285027613977733Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG3697231.8731866191358453Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC3164381.6032203227446185No Hit
GGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT2140581.0845161954192213No Hit
GCTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG993050.5031247642513047Illumina Small RNA Adapter 1 (100% over 26bp)
GCAAATCTCCTAGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA903850.4579319451875955Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT895860.45388384401809967No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT893770.452824953975015Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGGATCGT735170.3724709057294514No Hit
GGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC647300.32795192578406884Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACCCTTATCAGTTCTCCGTCCAGATCGTCGGACTGTAGAACTCTGAACGT627240.31778860795427055Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGATCG556970.28218659679275887No Hit
AAGATGTTAAAGTTCATCAATAGTTCGATAGATCGTCGGACTGTAGAACT507940.2573457456863277No Hit
AATCTTCATAAAGAACACAAGAGACATAGAGATCGTCGGACTGTAGAACT499660.2531507171902793No Hit
GCAAATCTCCTAGTCGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG384910.19501309401134856Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGTGGATC353020.1788561545501189No Hit
GCAAATCTCCTAGTCGAAAGGCTTAGTCTCAATAGATCGCAGTGATCGTC334020.1692298814311674No Hit
GGGTTGCAATGTAATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT298640.15130474759177245No Hit
GTGGCAGATGCCGTCAAATGCCGACAGACAGATCGTCGGACTGTAGAACT275230.13944416581731695No Hit
GGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC257930.1306791908195348Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTCACTCAAGCCAGCTGTGATAGATCGTCGGACTGTAGAACTCTGAACG254400.12889073060322434Illumina Small RNA Adapter 1 (100% over 26bp)
AACTTCAAATATCAGAGAGAACATGGAAAAGATCGTCGGACTGTAGAACT238610.12089079099542199No Hit
ACTACTATTGTACAAAGCGATAAAGACACAGATCGTCGGACTGTAGAACT237360.12025748355338572No Hit
TGGTTGCAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACT234420.11876794444971638No Hit
AGCTTAAATGTGATAAAAAGTTCAGATCAGATCGTCGGACTGTAGAACTC231320.11719734199346639Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTCCTAGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT227510.11526702091013981Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATATTCACAGATCCTGATGATATAGCCAGATCGTCGGACTGTAGAACTCT224170.11357482342501884Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCTTTGGACATCATTGTCTTAACGATCGGAGATCGTCGGACTGTAGAACT211300.10705429000181328No Hit
GTTCAGTGAGATTTGTCAACATGAAAATTCTGACCCGAAAGATGGACATC210820.10681109994407136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)