FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_ATCACG_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ATCACG_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33409414
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC31824949.525740259915963TruSeq Adapter, Index 1 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT1899420.5685283794561616TruSeq Adapter, Index 1 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1833620.548833331826772TruSeq Adapter, Index 1 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1026100.30712900262183584TruSeq Adapter, Index 1 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC714990.21400854262214833TruSeq Adapter, Index 1 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC457080.13681173815260572TruSeq Adapter, Index 1 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC428340.1282093723643282TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGATCTCGTATGC384910.11521004229526444TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA260500.079.978031
CCGGAAG276850.079.120183
ACCGGAA290700.075.174552
GAGCACA5730400.072.81159
AGAGCAC5790650.072.0909358
AAGAGCA5896800.070.862487
GAAGAGC5901100.070.842236
GGAAGAG5964550.068.7170945
CGGAAGA5851600.067.909094
TCGGAAG5375100.067.3643953
ATCGGAA5505000.065.722842
GATCGGA5545000.063.9385071
AATCATA3350100.061.4927065
ATCATAA3554350.059.8561256
CATAAAC3558500.059.251098
TCATAAA3575500.059.083627
TCCGAAG87650.058.4798363
TAAATCA3228450.058.273443
ACGGAAG150700.058.0963333
CATCGGA92500.058.0424161
Produced by FastQC (version 0.11.4)