FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_CAGATC_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39780221
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC570949214.352589946647104TruSeq Adapter, Index 7 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3284100.8255610244095929TruSeq Adapter, Index 7 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT3061650.7696412747430438TruSeq Adapter, Index 7 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1839850.4625037151000242TruSeq Adapter, Index 7 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1288310.323856923771238TruSeq Adapter, Index 7 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC776890.19529554649784375TruSeq Adapter, Index 7 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC771830.19402355758657047TruSeq Adapter, Index 7 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC761820.1915072316968777TruSeq Adapter, Index 7 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT526660.13239242687967973TruSeq Adapter, Index 7 (100% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC475530.11953930572683345No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGATCATCTCGTATGC437480.10997425077150778TruSeq Adapter, Index 7 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA426750.085.4515461
CCGGAAG441550.085.125943
ACCGGAA459200.081.888142
GAGCACA10090550.071.6436849
AGAGCAC10205450.070.884638
TCCGAAG137250.070.195813
AAGAGCA10390750.069.703737
GAAGAGC10433000.069.456236
ACGGAAG216100.069.3146063
GATTGGA297750.068.569131
CGAAGAG166200.068.530635
TTGGAAG300200.068.474963
CATCGGA137700.067.629661
GGAAGAG10486950.067.628015
ATTGGAA307400.066.966372
CGGAAGA10368250.066.35184
TCGGAAG9598250.065.370353
TGGAAGA339000.065.341994
ATCGGAA9851950.063.6368262
CCGAAGA179700.062.7565774
Produced by FastQC (version 0.11.4)