FastQCFastQC Report
Thu 27 Apr 2017
trimmed-2112_lane1_ACAGTG_L001_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed-2112_lane1_ACAGTG_L001_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12260444
Sequences flagged as poor quality0
Sequence length1-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC540310.4406936649276323No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA284730.2322346564284295No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT165960.13536214512296618No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG154150.12572954127925548No Hit
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA126360.10306315170967707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA615350.059.1745575
TCATAAA680500.054.7998547
ATCATAA703550.053.5527046
TAAATCA651550.051.1655243
AAATCAT722450.049.5980724
CATAAAC829300.045.228558
AATAAAT756750.043.925621
AGAGCAC118300.040.86177494-95
ATAAACA958900.039.3458069
ATAAATC951750.035.005212
AACCGGG13500.031.5634121
ACCGGGC14250.030.5373842
AAGAGCA180900.029.95039694-95
AGCCCCC17550.029.3377881
CACGGGC19200.029.1464653
GAGCACA78900.028.83556794-95
AGCCGCC21650.028.4972781
AAACGGG686100.027.695614-15
GCCCGTA19200.027.0585962
CAAACGG683750.026.97044214-15
Produced by FastQC (version 0.11.4)