FastQCFastQC Report
Thu 27 Apr 2017
trimmed-2112_lane1_ATCACG_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed-2112_lane1_ATCACG_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28381961
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA3253150.059.5541425
ATCATAA3467150.057.724136
CATAAAC3480500.056.9874048
TCATAAA3500450.056.7911957
TAAATCA3134250.056.472893
AAATCAT3428850.055.302854
ATAAATC3267350.054.0666472
ATAAACA4449200.044.72649
AATAAAT4137900.042.6462751
AGAGCAC186350.039.02046294-95
AAACGGG2843050.035.4009114-15
ACGGGCA2862550.035.0124216-17
GGGCAAC2867950.034.50932318-19
CAAACGG2853300.034.3372614-15
AACGGGC2846750.033.6533716-17
CGGGCAA2858600.033.27123318-19
GCAACAT3004350.033.01428220-21
ACAAACG3152050.032.4781612-13
GGCAACA2962150.031.8666420-21
CATACCT3234250.030.10000224-25
Produced by FastQC (version 0.11.4)