FastQCFastQC Report
Thu 27 Apr 2017
trimmed-2112_lane1_CAGATC_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed-2112_lane1_CAGATC_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31266957
Sequences flagged as poor quality0
Sequence length1-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC411390.13157340511262416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA3159750.057.4435045
TCATAAA3371900.055.3232577
ATCATAA3472350.054.1242876
TAAATCA3126500.053.1100043
AAATCAT3357950.052.9559944
CATAAAC3618150.051.5414128
ATAAATC3437700.048.1428072
AGAGCAC335800.043.7741994-95
AATAAAT3799700.043.469081
ATAAACA4522250.041.3920869
ACCGGGC29400.040.1905862
AACCGGG30100.038.3502661
CACGGGC39300.037.132673
CCGGGCA34900.035.272683
TACGGCG33650.034.598296
GAGCACA207850.034.28111694-95
CACACGG41550.033.4891781
ATACGGC34800.033.1932955
CCAACGG35150.032.342692
AAACGGG2964550.032.0597114-15
Produced by FastQC (version 0.11.4)