FastQCFastQC Report
Thu 27 Apr 2017
trimmed-2112_lane1_GCCAAT_L001_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed-2112_lane1_GCCAAT_L001_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20811265
Sequences flagged as poor quality0
Sequence length1-101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA3399500.063.483575
ATAAATC3168750.062.210852
TAAATCA3182450.062.072933
TCATAAA3603650.061.5008967
ATCATAA3637700.061.4026536
AAATCAT3483050.060.6564984
CATAAAC3649250.060.5678988
AATAAAT3848500.051.1438331
ATAAACA4454500.049.7116179
CACACGG26650.042.2411041
AACCGGG19550.040.8349461
ACACGGG29300.038.4361342
CCAACGG25900.036.8989032
ACCGGGC23050.035.4269752
AGAGCAC159200.035.3967494-95
AAACGGG3290900.034.27490614-15
CACGGGC33650.034.2745483
ACAAACG3375150.033.88994612-13
CAAACGG3283400.033.27822514-15
ACGGGCA3375950.033.25123616-17
Produced by FastQC (version 0.11.4)