FastQCFastQC Report
Thu 27 Apr 2017
trimmed-2112_lane1_TTAGGC_L001_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed-2112_lane1_TTAGGC_L001_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21462840
Sequences flagged as poor quality0
Sequence length1-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA2137100.054.9154435
TCATAAA2296000.052.26197
ATCATAA2326450.051.9783866
TAAATCA2087400.051.537563
AAATCAT2297250.050.1248864
CATAAAC2447050.048.9688228
AGAGCAC276350.048.70750894-95
ATAAATC2312350.046.412292
AACCGGG16300.046.016251
CACGGGC23350.042.0876543
CCGGGCA19350.041.7846263
GAGCACA155350.041.5445994-95
CACACGG22750.041.01611
ACCGGGC18950.040.7744182
AATAAAT2648950.040.459221
ATAAACA3144200.038.3115849
AGCCCGA14400.037.2055971
CCAACGG22100.037.185742
AGCCCCC20300.036.7290731
ACACGGG26750.035.8976142
Produced by FastQC (version 0.11.4)