FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_ACAGTG_L001_R1_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ACAGTG_L001_R1_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408105
Sequences flagged as poor quality0
Sequence length20-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA917660.6844069314791315TruSeq Adapter, Index 5 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA660380.49252299262274574TruSeq Adapter, Index 5 (98% over 63bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC573360.4276219495596134No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA407510.3039281091548731TruSeq Adapter, Index 5 (98% over 63bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA302840.22586338636220404No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA301050.22452837295053998TruSeq Adapter, Index 5 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA269060.20066966957672244TruSeq Adapter, Index 5 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAA216870.16174545172490817TruSeq Adapter, Index 5 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAA212140.158217734720902TruSeq Adapter, Index 5 (100% over 60bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA203410.15170674752323315TruSeq Adapter, Index 5 (100% over 60bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT186010.13872952218080034TruSeq Adapter, Index 5 (100% over 50bp)
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT183330.1367307311510463No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC172270.1284819890655689TruSeq Adapter, Index 5 (98% over 50bp)
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG171540.1279375422552255No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA164430.12263477948599TruSeq Adapter, Index 5 (98% over 63bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA147840.1102616663577739TruSeq Adapter, Index 5 (98% over 63bp)
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA137300.10240074939747265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA224000.082.27941
CCGGAAG236500.081.027353
ACCGGAA242750.078.779022
ACGGAAG108850.071.269633
CATCGGA75250.069.3260651
TCGGAAG126700.068.043683
ATTGGAA155400.067.78112
TTGGAAG157850.066.9513243
ATCGGAA122850.066.376032
GATTGGA159050.065.599321
TGGAAGA173500.064.7662664
CGAAGAG69200.063.7710275
TCCGAAG59550.062.5098533
GAGCGGA73200.061.7572021
GAACGGA123300.060.5343171
TAAGAGC44400.060.5229953
GCGGAAG79100.060.426413
CCGAAGA72950.060.08084
AGCGGAA80200.059.4886472
AACGGAA130550.059.154132
Produced by FastQC (version 0.11.4)