FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_ATCACG_R1_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ATCACG_R1_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29637631
Sequences flagged as poor quality0
Sequence length20-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAA775880.2617888049149407TruSeq Adapter, Index 1 (100% over 60bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAA343780.11599442614019995TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA255450.079.105851
CCGGAAG272550.077.9142153
ACCGGAA285500.074.320392
TCGGAAG140750.063.06953
ACGGAAG132950.062.0609363
GAAGAGC1003900.061.6276556
TCCGAAG78950.060.754533
CATCGGA86750.060.649691
CGAAGAG96350.059.959295
ATCGGAA145200.059.340252
AATCATA3291400.059.3075525
GGAAGAG938300.058.061645
CGGAAGA741500.057.5460554
ATCATAA3513350.057.5330246
AGAGCAC1079550.057.338268
AAGAGCA1088000.056.8515557
TCATAAA3548750.056.583547
CATAAAC3544200.056.489758
TAAATCA3163200.056.2650223
GAACGGA138250.055.8471341
Produced by FastQC (version 0.11.4)