FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_CAGATC_R1_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_R1_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33309531
Sequences flagged as poor quality0
Sequence length20-101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA1251630.3757573170273697TruSeq Adapter, Index 7 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA1106020.33204310201785786TruSeq Adapter, Index 7 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA656310.19703369585119648TruSeq Adapter, Index 7 (98% over 63bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC435860.13085143708567978No Hit
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA431830.12964157315814503TruSeq Adapter, Index 7 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAA348840.10472678225340369TruSeq Adapter, Index 7 (100% over 60bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA414700.083.307471
CCGGAAG434500.082.399153
ACCGGAA450900.079.416212
ACGGAAG197800.071.25243
TCCGAAG124200.070.8911363
CGAAGAG149050.070.5352555
TCGGAAG202650.070.2067643
CCGAAGA154200.067.467694
TTGGAAG285350.067.379773
GATTGGA284650.067.244411
GAGCGGA124800.067.027631
GCGGAAG132450.066.408213
CATCGGA127250.066.3325041
ATCGGAA209500.066.000492
TGGAAGA315950.065.0375754
ATTGGAA299900.064.203282
GAAGAGC1668200.063.58226
AGCGGAA139050.063.5158462
GAACGGA214850.063.4939271
GGAAGAG1493900.061.419835
Produced by FastQC (version 0.11.4)