Basic Statistics
Measure | Value |
---|---|
Filename | 2112_lane1_GCCAAT_L001_R1_trimmed.fq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 21885902 |
Sequences flagged as poor quality | 0 |
Sequence length | 20-101 |
%GC | 35 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA | 72143 | 0.32963229022957335 | TruSeq Adapter, Index 6 (100% over 60bp) |
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA | 54280 | 0.24801353857839625 | TruSeq Adapter, Index 6 (98% over 63bp) |
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA | 29200 | 0.1334192211954527 | TruSeq Adapter, Index 6 (98% over 63bp) |
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA | 26054 | 0.1190446708570659 | TruSeq Adapter, Index 6 (98% over 63bp) |
AATAAATCATAAACAAACGGGCAACATACCTAAAACTCAAAAAATTCATC | 22271 | 0.10175957106999749 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GACCGGA | 18885 | 0.0 | 83.63903 | 1 |
CCGGAAG | 20335 | 0.0 | 81.36318 | 3 |
ACCGGAA | 20930 | 0.0 | 79.246475 | 2 |
ACGGAAG | 9475 | 0.0 | 70.38043 | 3 |
TCGGAAG | 10495 | 0.0 | 70.15184 | 3 |
GAACGGA | 9180 | 0.0 | 68.922714 | 1 |
CATCGGA | 6660 | 0.0 | 67.461945 | 1 |
ATCGGAA | 10510 | 0.0 | 67.224365 | 2 |
GATTGGA | 13270 | 0.0 | 66.663376 | 1 |
TCCGAAG | 6075 | 0.0 | 66.3072 | 3 |
GAGCGGA | 6065 | 0.0 | 64.420815 | 1 |
AATCATA | 342770 | 0.0 | 63.30617 | 5 |
TAAATCA | 317315 | 0.0 | 62.374973 | 3 |
ATAAATC | 317630 | 0.0 | 62.171833 | 2 |
TCATAAA | 363020 | 0.0 | 61.422333 | 7 |
ATCATAA | 366565 | 0.0 | 61.28671 | 6 |
AAATCAT | 352625 | 0.0 | 60.349003 | 4 |
TTGGAAG | 14905 | 0.0 | 60.278526 | 3 |
CATAAAC | 369810 | 0.0 | 60.163017 | 8 |
TGGAAGA | 16425 | 0.0 | 59.24969 | 4 |