FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_GCCAAT_L001_R1_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_GCCAAT_L001_R1_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21885902
Sequences flagged as poor quality0
Sequence length20-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA721430.32963229022957335TruSeq Adapter, Index 6 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA542800.24801353857839625TruSeq Adapter, Index 6 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA292000.1334192211954527TruSeq Adapter, Index 6 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA260540.1190446708570659TruSeq Adapter, Index 6 (98% over 63bp)
AATAAATCATAAACAAACGGGCAACATACCTAAAACTCAAAAAATTCATC222710.10175957106999749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA188850.083.639031
CCGGAAG203350.081.363183
ACCGGAA209300.079.2464752
ACGGAAG94750.070.380433
TCGGAAG104950.070.151843
GAACGGA91800.068.9227141
CATCGGA66600.067.4619451
ATCGGAA105100.067.2243652
GATTGGA132700.066.6633761
TCCGAAG60750.066.30723
GAGCGGA60650.064.4208151
AATCATA3427700.063.306175
TAAATCA3173150.062.3749733
ATAAATC3176300.062.1718332
TCATAAA3630200.061.4223337
ATCATAA3665650.061.286716
AAATCAT3526250.060.3490034
TTGGAAG149050.060.2785263
CATAAAC3698100.060.1630178
TGGAAGA164250.059.249694
Produced by FastQC (version 0.11.4)