Basic Statistics
Measure | Value |
---|---|
Filename | 2112_lane1_TTAGGC_L001_R1_trimmed.fq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22518331 |
Sequences flagged as poor quality | 0 |
Sequence length | 20-101 |
%GC | 36 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA | 69931 | 0.3105514347399903 | TruSeq Adapter, Index 3 (100% over 60bp) |
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA | 26645 | 0.11832582086123523 | TruSeq Adapter, Index 3 (98% over 63bp) |
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA | 23772 | 0.10556732645949649 | TruSeq Adapter, Index 3 (98% over 63bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GACCGGA | 20815 | 0.0 | 83.41447 | 1 |
CCGGAAG | 21520 | 0.0 | 83.21455 | 3 |
ACCGGAA | 22750 | 0.0 | 78.994095 | 2 |
ACGGAAG | 10005 | 0.0 | 72.20067 | 3 |
CGAAGAG | 6980 | 0.0 | 69.91323 | 5 |
TCCGAAG | 5890 | 0.0 | 68.35347 | 3 |
TCGGAAG | 10535 | 0.0 | 68.27244 | 3 |
CATCGGA | 6345 | 0.0 | 67.95473 | 1 |
GCGGAAG | 6715 | 0.0 | 65.792 | 3 |
GAGCGGA | 6410 | 0.0 | 65.31994 | 1 |
TGGAAGA | 16810 | 0.0 | 63.79159 | 4 |
GAAGAGC | 83915 | 0.0 | 63.416653 | 6 |
AGCGGAA | 7030 | 0.0 | 62.97433 | 2 |
ATCGGAA | 10800 | 0.0 | 62.807087 | 2 |
TTGGAAG | 16225 | 0.0 | 61.677364 | 3 |
CCGAAGA | 7785 | 0.0 | 61.60724 | 4 |
GAACGGA | 11195 | 0.0 | 61.59178 | 1 |
ATTGGAA | 16270 | 0.0 | 61.56508 | 2 |
GATTGGA | 16200 | 0.0 | 61.204887 | 1 |
GGAAGAG | 76915 | 0.0 | 60.482033 | 5 |