FastQCFastQC Report
Fri 21 Apr 2017
2112_lane1_TTAGGC_L001_R1_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TTAGGC_L001_R1_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22518331
Sequences flagged as poor quality0
Sequence length20-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA699310.3105514347399903TruSeq Adapter, Index 3 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA266450.11832582086123523TruSeq Adapter, Index 3 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA237720.10556732645949649TruSeq Adapter, Index 3 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA208150.083.414471
CCGGAAG215200.083.214553
ACCGGAA227500.078.9940952
ACGGAAG100050.072.200673
CGAAGAG69800.069.913235
TCCGAAG58900.068.353473
TCGGAAG105350.068.272443
CATCGGA63450.067.954731
GCGGAAG67150.065.7923
GAGCGGA64100.065.319941
TGGAAGA168100.063.791594
GAAGAGC839150.063.4166536
AGCGGAA70300.062.974332
ATCGGAA108000.062.8070872
TTGGAAG162250.061.6773643
CCGAAGA77850.061.607244
GAACGGA111950.061.591781
ATTGGAA162700.061.565082
GATTGGA162000.061.2048871
GGAAGAG769150.060.4820335
Produced by FastQC (version 0.11.4)