Bismark report for: trimmed-2112_lane1_ACAGTG_L001_R1.fastq (version: v0.16.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12260444 Number of alignments with a unique best hit from the different alignments: 819748 Mapping efficiency: 6.7% Sequences with no alignments under any condition: 10640673 Sequences did not map uniquely: 800023 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 421342 ((converted) top strand) CT/GA: 398406 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 17032078 Total methylated C's in CpG context: 2827873 Total methylated C's in CHG context: 2537021 Total methylated C's in CHH context: 8087889 Total methylated C's in Unknown context: 1 Total unmethylated C's in CpG context: 320249 Total unmethylated C's in CHG context: 1299451 Total unmethylated C's in CHH context: 1959595 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 89.8% C methylated in CHG context: 66.1% C methylated in CHH context: 80.5% C methylated in Unknown context (CN or CHN): 100.0%