Bismark report for: trimmed-2112_lane1_ATCACG_R1.fastq (version: v0.16.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 28381961 Number of alignments with a unique best hit from the different alignments: 2329743 Mapping efficiency: 8.2% Sequences with no alignments under any condition: 24855997 Sequences did not map uniquely: 1196221 Sequences which were discarded because genomic sequence could not be extracted: 2 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 1192367 ((converted) top strand) CT/GA: 1137374 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 45065008 Total methylated C's in CpG context: 5617085 Total methylated C's in CHG context: 7145664 Total methylated C's in CHH context: 24093281 Total methylated C's in Unknown context: 1 Total unmethylated C's in CpG context: 840918 Total unmethylated C's in CHG context: 2324719 Total unmethylated C's in CHH context: 5043341 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 87.0% C methylated in CHG context: 75.5% C methylated in CHH context: 82.7% C methylated in Unknown context (CN or CHN): 100.0%