Bismark report for: trimmed-2112_lane1_TTAGGC_L001_R1.fastq (version: v0.16.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 21462840 Number of alignments with a unique best hit from the different alignments: 904131 Mapping efficiency: 4.2% Sequences with no alignments under any condition: 19934450 Sequences did not map uniquely: 624259 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 462275 ((converted) top strand) CT/GA: 441856 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 17897568 Total methylated C's in CpG context: 2616074 Total methylated C's in CHG context: 2381310 Total methylated C's in CHH context: 7596641 Total methylated C's in Unknown context: 1 Total unmethylated C's in CpG context: 484199 Total unmethylated C's in CHG context: 1661537 Total unmethylated C's in CHH context: 3157807 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 84.4% C methylated in CHG context: 58.9% C methylated in CHH context: 70.6% C methylated in Unknown context (CN or CHN): 100.0%