Bismark report for: hard-trimmed-2112_lane1_TGACCA_L001_R1_001.fastq (version: v0.16.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 2800289 Number of alignments with a unique best hit from the different alignments: 623273 Mapping efficiency: 22.3% Sequences with no alignments under any condition: 1588744 Sequences did not map uniquely: 588272 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 59279 ((converted) top strand) CT/GA: 55305 ((converted) bottom strand) GA/CT: 258437 (complementary to (converted) top strand) GA/GA: 250252 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 10412368 Total methylated C's in CpG context: 2215432 Total methylated C's in CHG context: 1428445 Total methylated C's in CHH context: 3781284 Total methylated C's in Unknown context: 3 Total unmethylated C's in CpG context: 261842 Total unmethylated C's in CHG context: 1215969 Total unmethylated C's in CHH context: 1509396 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 89.4% C methylated in CHG context: 54.0% C methylated in CHH context: 71.5% C methylated in Unknown context (CN or CHN): 100.0%