Bismark report for: trimmed-2112_lane1_ACAGTG_L001_R1.fastq (version: v0.16.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12260444 Number of alignments with a unique best hit from the different alignments: 3439005 Mapping efficiency: 28.0% Sequences with no alignments under any condition: 6508309 Sequences did not map uniquely: 2313130 Sequences which were discarded because genomic sequence could not be extracted: 4 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 194297 ((converted) top strand) CT/GA: 177441 ((converted) bottom strand) GA/CT: 1574633 (complementary to (converted) top strand) GA/GA: 1492630 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 72855963 Total methylated C's in CpG context: 15487306 Total methylated C's in CHG context: 5008319 Total methylated C's in CHH context: 15081081 Total methylated C's in Unknown context: 5 Total unmethylated C's in CpG context: 2557484 Total unmethylated C's in CHG context: 14183192 Total unmethylated C's in CHH context: 20538581 Total unmethylated C's in Unknown context: 78 C methylated in CpG context: 85.8% C methylated in CHG context: 26.1% C methylated in CHH context: 42.3% C methylated in Unknown context (CN or CHN): 6.0%