Trimming

Input Reads

../oly_pacbio_.correctedReads.fasta.gz
Parameterspreset = N/A
defaultQV = 20
isNonRandom = false
removeDuplicateReads = true
finalTrim = true
removeSpurReads = true
removeChimericReads = true
checkForSubReads = true
FASTA603105 reads (2821269801 bp)
0 reads (0 bp) were short and not loaded
FASTQ0 reads (0 bp)
0 reads (0 bp) were short and not loaded
603105 reads (2821269801 bp) loaded, 0 reads (0 bp) skipped, 0 warnings

Final Store

trimming/oly_pacbio_.gkpStore
readsLoaded603105 reads (2821269801 bp)
readsSkipped0 reads (0 bp) (read was too short)
warnings0 warnings (invalid base or quality value)

Read Length Histogram

k-Mer Counts

Histogram for k=22 with 2808604596 mers, 795486248 distinct mers and 499876935 single-copy mers. Largest count is 977988.

Overlaps

No statistics available for store 'trimming/oly_pacbio_.ovlStore'.

Trimming

PARAMETERS
1000reads trimmed below this many bases are deleted
0.0450use overlaps at or below this fraction error
1break region if overlap is less than this long, for 'largest covered' algorithm
1break region if overlap coverage is less than this many read, for 'largest covered' algorithm
INPUT READS
readsbases
6031052821269801reads processed
00reads not processed, previously deleted
00reads not processed, in a library where trimming isn't allowed
OUTPUT READS
readsbases
4746952067034122trimmed reads output
57213285060868reads with no change, kept as is
3209366698380reads with no overlaps, deleted
3910493466994reads with short trimmed length, deleted
TRIMMING DETAILS
readsbases
392462160713464bases trimmed from the 5' end of a read
367569148295973bases trimmed from the 3' end of a read

Image 'trimming/3-overlapbasedtrimming/oly_pacbio_.1.trimReads.inputDeletedReads' is empty. Probably no data to display.

Image 'trimming/3-overlapbasedtrimming/oly_pacbio_.1.trimReads.inputNoTrimReads' is empty. Probably no data to display.

Splitting

PARAMETERS
1000reads trimmed below this many bases are deleted
0.0450use overlaps at or below this fraction error
INPUT READS
readsbases
5319082661104427reads processed
71197160165374reads not processed, previously deleted
00reads not processed, in a library where trimming isn't allowed
PROCESSED
readsbases
00no overlaps
19125844753no coverage after adjusting for trimming done already
00processed for chimera
00processed for spur
5299962655259674processed for subreads
READS WITH SIGNALS
readssignals
00number of 5' spur signal
00number of 3' spur signal
00number of chimera signal
491497number of subread signal
SIGNALS
readsbases
00size of 5' spur signal
00size of 3' spur signal
00size of chimera signal
497194985size of subread signal
TRIMMING
readsbases
240660327trimmed from the 5' end of the read
253708286trimmed from the 3' end of the read