Bismark report for: hard-trimmed-2112_lane1_ACAGTG_L001_R1.fastq (version: v0.16.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/srlab/Documents/C-virginica-BSSeq/genome/ with the specified options: -q -N 1 --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12197930 Number of alignments with a unique best hit from the different alignments: 3456338 Mapping efficiency: 28.3% Sequences with no alignments under any condition: 5760842 Sequences did not map uniquely: 2980750 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 181719 ((converted) top strand) CT/GA: 166362 ((converted) bottom strand) GA/CT: 1588675 (complementary to (converted) top strand) GA/GA: 1519582 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 61813350 Total methylated C's in CpG context: 12572131 Total methylated C's in CHG context: 4005979 Total methylated C's in CHH context: 12350257 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 2271987 Total unmethylated C's in CHG context: 12442077 Total unmethylated C's in CHH context: 18170919 Total unmethylated C's in Unknown context: 5 C methylated in CpG context: 84.7% C methylated in CHG context: 24.4% C methylated in CHH context: 40.5% C methylated in Unknown context (CN or CHN): 0.0%