FastQCFastQC Report
Sat 31 Dec 2016
EPI-103_S27_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-103_S27_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20350323
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG566270.27826093964208826No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG298680.1467691692166262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGC1150.072.177211
CTAGGAC850.071.953741
CCTAGGT7900.071.33571
CTTAGGC1300.070.570021
CTATGGC2000.069.8979341
CCGTAGC750.069.897931
CCTGGTC700.068.649751
ACTAGGC454.7002686E-967.956321
CCATGGT6450.067.730551
CCTAGAA850.066.814191
CCTAGCA200.002891021865.5293051
CTCTGGT9250.065.1750951
CCTAGGA2750.065.1321641
CTGTGGC2150.065.021331
CCAAAGG700.062.4088631
CTGTACG1700.061.674641
ACCTGGT7900.061.3818781
CCGACGT5700.061.313971
CCAAGGT3500.061.1606861
CTACGCG1500.061.1606831
Produced by FastQC (version 0.11.4)