FastQCFastQC Report
Sat 31 Dec 2016
EPI-103_S27_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-103_S27_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20350323
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA508990.2501139662500689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT42200.076.894031
GGGCCCA800.076.325591
GGGACCA356.494338E-874.767941
GACCTGC3450.074.587331
ATGCCCT13900.073.1094061
GGCCCCT10800.073.094891
GGTCCGC302.272045E-672.691041
CGCCAGT900.072.691041
CCGCAGA3050.071.499391
GGACCCA3300.071.369391
GGACCCT2150.071.000561
CACGGAT1850.070.7267762
GCTCTGA750.069.78341
GGACCGA750.069.78341
GGATAAC3700.069.547145
GGCCCCA8300.069.363021
GACCTGT1450.069.181821
GCCCAGA2950.068.009251
CATGGAT1100.067.4047552
GTCCAGA1750.067.291141
Produced by FastQC (version 0.11.4)