FastQCFastQC Report
Sat 31 Dec 2016
EPI-104_S28_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-104_S28_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30016263
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG512030.17058419297565458No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG358200.11933530832935466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGGT52200.057.186241
CCATGGT12650.055.0277751
CCGTAGC1400.054.9072041
CCGTACG3600.054.568271
CTCGCGA2050.054.1632041
CCTAGGT15800.054.0577241
CCTGGGT60800.053.5224841
CTAAGCG1550.052.3488041
CAGTGGT36000.052.3143651
CCGTAAG3850.052.1341131
CCGACGT9850.052.027131
CCGTAGT13700.051.7454761
CATGGGT36000.051.7212331
CTTGGGT49000.051.6824951
ACTGGGT36200.051.553451
CTGGTAA66950.051.0936361
CTATGGC3700.050.785041
CTTAGCG3450.050.7515871
CTACGCG3050.050.4066161
CTCTGGT17900.050.3401261
Produced by FastQC (version 0.11.4)