FastQCFastQC Report
Sat 31 Dec 2016
EPI-104_S28_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-104_S28_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30016263
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA684530.2280530391141629No Hit
TCTACATAAACCAAACTCAAAAACTAAATATCACTAAACAAACCAAAAAA307000.10227788848998293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAT63300.073.905031
CGCAGAT5350.067.7544252
GGCCCCT16850.066.055911
ATGCCCT20750.064.122081
CTGCATA80200.063.0110132
GACCTGC5550.060.7023051
AGCCCGA3650.059.5865671
GGCCCCA14250.059.254511
CCGCAGA5050.059.112281
GCCCCGT7850.057.584721
GGTGTGC24100.057.5048986
AGCCGCC2600.057.407121
TGCATAA86650.057.385643
TGTGCTC31150.057.3594978
CACGGAT3450.056.8605772
GCAGATA9850.056.716363
CCCAGTA24050.056.5650372
GTGCTCT31650.056.4538159
TGCCCCT25600.055.971941
GTGTGCT32000.055.7027177
Produced by FastQC (version 0.11.4)