FastQCFastQC Report
Sat 31 Dec 2016
EPI-111_S29_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-111_S29_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27015750
Sequences flagged as poor quality0
Sequence length20-101
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG812930.3009096545533624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGT9250.069.059271
CCTAGGT11600.064.550871
CCATGGG4600.062.537131
CTATGGC2700.061.1065371
CCAGTGT5200.061.016141
CCAAGGT4100.060.8772431
ACTGGTG18600.060.500021
CTAAGGC2450.060.435031
CCGACGG3050.059.6424451
ACTGGGT34350.059.36181
CTGTGGT45050.059.16061
CCATGTA3950.058.9050331
CCTGGGA20200.058.221081
CCGAACG800.058.1687241
CCTTGGT10200.058.0650331
CACTGGT4300.058.045741
CTCTGGT16500.057.9443781
CCGTGGC2200.057.6879841
CTCGTGT2350.057.606161
CAGTGGT31150.056.768191
Produced by FastQC (version 0.11.4)