FastQCFastQC Report
Sat 31 Dec 2016
EPI-111_S29_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-111_S29_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27015750
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA656260.2429175573508046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCA3250.075.409591
TCTGCAT53600.072.527881
ATGCCCT21850.069.233061
GGCCCCA11750.068.687461
CCGCAGA4650.068.153831
GACCTGC3750.067.60861
GGACCCT2200.067.224461
GGCCCCT15450.067.0069051
GGTCCCA3800.066.719011
CACGGAT2800.066.401552
GGCCCGA2800.066.4013061
CGCTAGT700.066.4013061
GGATAAC4350.066.051935
GGACCGA1350.065.730581
GACCAGA4050.065.7305761
GTGCCCT3550.065.466071
GCCCAGT3900.065.008261
GCAGATA8150.064.808263
GGGCCCA1050.064.389141
AGCCCCT17450.064.170061
Produced by FastQC (version 0.11.4)