FastQCFastQC Report
Sat 31 Dec 2016
EPI-113_S30_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-113_S30_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26303054
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG627170.23843999255751822No Hit
GGTAATATTTGGGTTTTAATGGATATTTTAGTTGTTTTTTATATTTTTTG322100.1224572629474889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAC302.5474947E-885.5708851
CTTAGGC1950.074.600261
ACCGCGT650.072.406131
CCTAGGT9800.072.036711
CTAGGTC1100.070.012541
CTATGGC2950.069.616991
CTGTGGC2500.068.45671
CCATGGT8800.067.581551
CTCTGGT13550.066.941061
CCAAGGT4200.065.196861
ACCTAGG1000.064.178161
CCCGTGC401.8698847E-764.1781541
GGGCCCG200.003139870264.1781541
CATGGAC1300.062.5325661
CTCTGGG13500.062.435051
CCAATGT1650.062.2333681
CCTGTGA2550.062.080841
CCTTGGT9250.061.981071
CATAGGC1450.061.9651221
CACTGGT4300.061.6906361
Produced by FastQC (version 0.11.4)