FastQCFastQC Report
Sat 31 Dec 2016
EPI-113_S30_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-113_S30_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26303054
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA579040.22014173715341193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTGC3350.076.524911
TCTGCAT51900.076.479441
GACTAGC456.548362E-1175.958051
GCAGATA7550.073.568373
CCGCAGA4900.071.5013351
GGACCCA3950.071.390961
CGCAGAT5450.071.341482
CCCGTGA1200.071.210681
GCCTAGC850.070.37291
ATGCCCT19350.069.996231
GTCCAGA1650.069.915941
GACCTGA2450.069.75741
GGATAAC5100.069.5328755
GGTCCCA3350.068.872411
GGTCCCT2300.068.733781
GGCCGCA2800.068.6674351
CTGCAGA3050.068.642421
GGACCGA1250.068.362251
GCTTAGA501.6734703E-1068.362241
CCATGGA1700.067.859591
Produced by FastQC (version 0.11.4)