FastQCFastQC Report
Sun 1 Jan 2017
EPI-119_S31_L005_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-119_S31_L005_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28906816
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAATATTTGGGTTTTAATGGATATGTTAGTTGTTTTTTATATTTTTTG448550.15517101572169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC550.078.4014741
CCATGGT11450.073.060591
CTAGCCG302.4310866E-671.868011
CTACGCG2050.071.517451
CCTAGGC1100.070.5613251
CCTAGGT13800.069.055791
CTATGGC2250.068.99331
ACCTAGA501.546141E-1068.99331
CTAGTTC258.52175E-568.99331
CTTAGGC1500.068.99331
CCGTAGC1450.068.398531
CCAAGGT5250.068.171941
CCGAGGC650.066.339711
CCATAGA1700.065.949481
CTGTAGC1250.065.543631
CATAGAC1200.064.681211
CATACGC601.0913936E-1164.681211
CTCAGGT3950.063.3166351
CCATGGG6100.062.207071
CCGATGT3700.061.767651
Produced by FastQC (version 0.11.4)