FastQCFastQC Report
Sun 1 Jan 2017
EPI-119_S31_L005_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEPI-119_S31_L005_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28906816
Sequences flagged as poor quality0
Sequence length20-101
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATAAACCACAATCAAAAACTAAATATCACTAAACAAACCAAAAAA445810.1542231423896703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAT5250.079.52922
GGACCCA4200.076.86431
TCTGCAT43250.074.8418351
GACCTGC3650.074.2951351
GCCCAGT3950.074.1010741
GGACCCC2000.073.174811
GCCTAGC1300.072.843711
GTTCAGA650.072.843711
CCGCAGA4750.072.495181
GGTCCGA950.072.495171
GCAGATA8150.071.299773
ATGCCCT17150.070.526921
GCCCAGA6000.069.5878141
GACCTGA2600.069.532631
GGGACCT258.597308E-568.8704151
GGTCCCA3450.068.620891
GGCCCCT14100.068.381971
GCCATGA4700.067.7714161
GCCATGT2500.067.148651
GGCCCCA12950.067.1421
Produced by FastQC (version 0.11.4)